Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis

The Investigation of Alpha-Tubulin Differential Expression in Oligodendroglioma Brain Tumor Aiming MALDI-TOF-TOF

International Clinical Neuroscience Journal ◽

10.15171/icnj.2019.11 ◽

2019 ◽

Vol 6 (2) ◽

pp. 53-58

Author(s):

Mehdi Pooladi ◽

Mehrdad Hashemi ◽

Saeed Hesami Tackallou ◽

Soheila Karani

Keyword(s):

Brain Tumor ◽

Protein Separation ◽

Expression Profiles ◽

Protein Detection ◽

Proteome Analysis ◽

Total Protein Content ◽

Structural Protein ◽

Alpha Tubulin ◽

Isoelectric Ph ◽

Protein Expression Profiles

Background: Tubulin is known as a heterodimer protein, which includes alpha and beta tubulin subunits. This structural protein plays important roles in pathogenesis and healing different diseases. Biomarkers help in fast and accurate detection of cancer. Proteomic studies can be useful both in biological and clinical research, also help obtain protein expression profiles by using twodimensional electrophoresis, mass spectrometry, and bioinformatics tools. Finding candidate proteins as cancer biomarkers is an interesting area in proteomic investigations. Methods: In the present study, the total protein content of healthy cells of the brain and brain tumor cells were extracted, purified and quantified by Bradford assay. Two-dimensional electrophoresis used for protein separation followed by statistical analysis. Primary protein detection was performed based on the differences in isoelectric pH, the molecular weight of proteins and protein data banks, which was further confirmed by Matrix Assisted Laser Desorption Ionisation-Time-of-Flight (MALDITOF-TOF). Results: In this study, an alpha-tubulin expression found changed (overexpression) in Oligodendroglioma tumors comparing control identified by proteomics analysis. Also, alphatubulin position showed in the oligodendroglioma tumors cluster diagram. Conclusion: Proteome analysis approach has allowed biology and medical studies. Alpha-tubulin introduced as a candidate biomarker for the diagnosis and prediction of oligodendroglioma tumors.

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Analysis of proteins copurifying with the cd4/lck complex using one-dimensional polyacrylamide gel electrophoresis and mass spectrometry: comparison with affinity-tag based protein detection and evaluation of different solubilization methods

Journal of the American Society for Mass Spectrometry ◽

10.1016/s1044-0305(03)00895-x ◽

2004 ◽

Author(s):

O Bernhard

Keyword(s):

Mass Spectrometry ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Protein Detection ◽

Polyacrylamide Gel ◽

Affinity Tag ◽

One Dimensional

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Proteome analysis of cerebrospinal fluid in patients with Multiple System Atrophy (MSA)

Aktuelle Neurologie ◽

10.1055/s-0028-1086803 ◽

2008 ◽

Vol 35 (S 01) ◽

Author(s):

S Werz ◽

V Lehmensiek ◽

S Süssmuth ◽

H Mogel ◽

J Brettschneider ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Multiple System Atrophy ◽

Proteome Analysis

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Protective effects of soy-isoflavones in cardiovascular disease

Hämostaseologie ◽

10.1055/s-0037-1616927 ◽

2008 ◽

Vol 28 (01/02) ◽

pp. 85-88 ◽

Cited By ~ 16

Author(s):

D. Fuchs ◽

H. Daniel ◽

U. Wenzel

Keyword(s):

Cardiovascular Disease ◽

Endothelial Cells ◽

Molecular Mechanisms ◽

Dietary Intervention ◽

Mononuclear Cells ◽

Oxidized Ldl ◽

Proteome Analysis ◽

Soy Isoflavones ◽

Protective Effects ◽

Estrogen Production

SummaryEpidemiological studies indicate that the consumption of soy-containing food may prevent or slow-down the development of cardiovascular disease. In endothelial cells application of a soy extract or a combination of the most abundant soy isoflavones genistein and daidzein both inhibited apoptosis, a driving force in atherosclerosis development, when applied in combination with oxidized LDL or homocysteine. Proteome analysis revealed that the stressorinduced alteration of protein expression profile was reversed by the soy extract or the genistein/daidzein mixture. Only few protein entities that could be functionally linked to mitochondrial dysfunction were regulated in common by both application forms of isoflavones. A dietary intervention with isoflavone-enriched soy extract in postmenopausal women, who generally show strongly increased cardiovascular risk due to diminished estrogen production, led to significant alterations in the steady state levels of proteins from mononuclear blood cells. The proteins identified by proteome analysis revealed that soy isoflavones may increase the anti-inflammatory response in blood mononuclear cells thereby contributing to the atherosclerosispreventive activities of a soy-rich diet. Conclusion: By proteome analysis protein targets were identified in vitro in endothelial cells that respond to soy isoflavones and that may decipher molecular mechanisms through which soy products exert their protective effects in the vasculature.

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