Fractional Viscoelastic Modeling Enabling Accurate Atomic Force Microscope Contact Resonance Spectroscopy Characterization

2021 ◽  
pp. 33-37
Author(s):  
Md Tasmirul Jalil ◽  
Rafiul Shihab ◽  
Ryan Tung
Keyword(s):  
Atomic Force Microscope ◽  
Resonance Spectroscopy ◽  
Atomic Force ◽  
Viscoelastic Modeling ◽  
Contact Resonance

scholarly journals Scanning speed phenomenon in contact-resonance atomic force microscopy

2018 ◽  
Vol 9 ◽  
pp. 945-952 ◽  
Author(s):  
Christopher C Glover ◽  
Jason P Killgore ◽  
Ryan C Tung
Keyword(s):  
Atomic Force Microscopy ◽  
Hydrodynamic Theory ◽  
Squeeze Film ◽  
Resonance Spectroscopy ◽  
Related Phenomenon ◽  
Contact Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Scan Speed ◽  
Contact Resonance

This work presents data confirming the existence of a scan speed related phenomenon in contact-mode atomic force microscopy (AFM). Specifically, contact-resonance spectroscopy is used to interrogate this phenomenon. Above a critical scan speed, a monotonic decrease in the recorded contact-resonance frequency is observed with increasing scan speed. Proper characterization and understanding of this phenomenon is necessary to conduct accurate quantitative imaging using contact-resonance AFM, and other contact-mode AFM techniques, at higher scan speeds. A squeeze film hydrodynamic theory is proposed to explain this phenomenon, and model predictions are compared against the experimental data.

Measuring forces between biological macromolecules with the Atomic Force Microscope: characterization and applications

1995 ◽  
Vol 53 ◽  
pp. 718-719
Author(s):  
Gil U Lee ◽  
Linda Chrisey ◽  
Richard J. Colton
Keyword(s):  
Atomic Force Microscope ◽  
Molecular Interactions ◽  
Molecular Interaction ◽  
Variable Stiffness ◽  
Force Transducer ◽  
Resonance Spectroscopy ◽  
Biological Macromolecules ◽  
Receptor System ◽  
Interaction Forces ◽  
Atomic Force

Structure and function in biological macromolecular systems such as proteins and polynucleotides are based on intermolecular interactions that are short ranged and chemically specific. Our knowledge of these molecular interactions results from indirect physical and thermodynamic measurements such as x-ray crystallography, light scattering and nuclear magnetic resonance spectroscopy. Direct measurement of molecular interaction forces requires that the state of a system be monitored with near atomic resolution while an independent force is applied to the system of 10−12 to 10−9 Newton magnitude. The atomic force microscope (AFM) has recently been applied to the study of single molecular interactions. The microfabricated cantilever of the AFM, a force transducer of small yet variable stiffness and high resonance frequency, produces a transducer of 10−15 N/Hz1/2 force sensitivities and 0.01 nm position accuracy.This presentation describes the AFM measurement of the molecular interaction forces in the model ligand-receptor system streptavidin-biotin and between complementary strands of DNA.

Continuous Measurement of Atomic Force Microscope Tip Wear by Contact Resonance Force Microscopy

Small ◽  
2011 ◽  
Vol 7 (8) ◽  
pp. 1018-1022 ◽  
Author(s):  
Jason P. Killgore ◽  
Roy H. Geiss ◽  
Donna C. Hurley
Keyword(s):  
Atomic Force Microscope ◽  
Continuous Measurement ◽  
Force Microscopy ◽  
Atomic Force ◽  
Contact Resonance

Nonlinear contact resonance spectroscopy in atomic force microscopy

2007 ◽  
Vol 40 (22) ◽  
pp. 7136-7145 ◽  
Author(s):  
Daniel Rupp ◽  
Ute Rabe ◽  
Sigrun Hirsekorn ◽  
Walter Arnold
Keyword(s):  
Atomic Force Microscopy ◽  
Resonance Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force ◽  
Nonlinear Contact ◽  
Contact Resonance

Characterization of photosystem II with the atomic-force microscope

1992 ◽  
Vol 50 (2) ◽  
pp. 1146-1147
Author(s):  
Kathleen M. Marr ◽  
Mary K. Lyon
Keyword(s):  
Photosystem Ii ◽  
Atomic Force Microscope ◽  
Three Dimensional ◽  
Biologically Active ◽  
Atomic Force ◽  
Freeze Etching ◽  
Image Averaging ◽  
Oxygen Evolving ◽  
Averaging Techniques

Photosystem II (PSII) is different from all other reaction centers in that it splits water to evolve oxygen and hydrogen ions. This unique ability to evolve oxygen is partly due to three oxygen evolving polypeptides (OEPs) associated with the PSII complex. Freeze etching on grana derived insideout membranes revealed that the OEPs contribute to the observed tetrameric nature of the PSIl particle; when the OEPs are removed, a distinct dimer emerges. Thus, the surface of the PSII complex changes dramatically upon removal of these polypeptides. The atomic force microscope (AFM) is ideal for examining surface topography. The instrument provides a topographical view of individual PSII complexes, giving relatively high resolution three-dimensional information without image averaging techniques. In addition, the use of a fluid cell allows a biologically active sample to be maintained under fully hydrated and physiologically buffered conditions. The OEPs associated with PSII may be sequentially removed, thereby changing the surface of the complex by one polypeptide at a time.

Imaging polymers, proteins and dna in aqueous solutions with the atomic force microscope

1989 ◽  
Vol 47 ◽  
pp. 32-33
Author(s):  
S.A.C. Gould ◽  
B. Drake ◽  
C.B. Prater ◽  
A.L. Weisenhorn ◽  
S.M. Lindsay ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Sequencing ◽  
Aqueous Solutions ◽  
Atomic Force Microscope ◽  
Piezoelectric Crystal ◽  
Atomic Force ◽  
Structure Of Molecules ◽  
Optical Lever

The atomic force microscope (AFM) is an instrument that can be used to image many samples of interest in biology and medicine. Images of polymerized amino acids, polyalanine and polyphenylalanine demonstrate the potential of the AFM for revealing the structure of molecules. Images of the protein fibrinogen which agree with TEM images demonstrate that the AFM can provide topographical data on larger molecules. Finally, images of DNA suggest the AFM may soon provide an easier and faster technique for DNA sequencing.The AFM consists of a microfabricated SiO2 triangular shaped cantilever with a diamond tip affixed at the elbow to act as a probe. The sample is mounted on a electronically driven piezoelectric crystal. It is then placed in contact with the tip and scanned. The topography of the surface causes minute deflections in the 100 μm long cantilever which are detected using an optical lever.

Sign in / Sign up

Export Citation Format

Share Document