scholarly journals Screening of Potato Cultivars (Solanum Tuberosum L.) and Identification of Markers of Fesistance Genes to PVX, PVY, Globodera Pallida and Globodera Rostochiensis

2021 ◽  
pp. 1-8
Author(s):  
Petr Fisenko ◽  
Olga Sobko ◽  
Irina Kim ◽  
Nathalia Matsishina ◽  
Dmitriy Volkov
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Globodera Rostochiensis ◽  
Potato Cultivars ◽  
Globodera Pallida

scholarly journals Genome-Wide Association Mapping of Late Blight Tolerance Trait in Potato (Solanum tuberosum L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Fang Wang ◽  
Meiling Zou ◽  
Long Zhao ◽  
Zhiqiang Xia ◽  
Jian Wang
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Potato Breeding ◽  
Genome Wide Association Study ◽  
Solanum Tuberosum L ◽  
Potato Cultivars ◽  
Genome Wide Association ◽  
Genome Wide ◽  
Population Structure Analysis ◽  
A Genome

Uncovering the genetic basis and optimizing the late blight tolerance trait in potatoes (Solanum tuberosum L.) are crucial for potato breeding. Late blight disease is one of the most significant diseases hindering potato production. The traits of late blight tolerance were evaluated for 284 potato cultivars to identify loci significantly associated with the late blight tolerance trait. Of all, 37 and 15 were the most tolerant to disease, and 107 and 30 were the most susceptible. A total of 22,489 high-quality single-nucleotide polymorphisms and indels were identified in 284 potato cultivars. All the potato cultivars were clustered into eight subgroups using population structure analysis and principal component analysis, which were consistent with the results of the phylogenetic tree analysis. The average genetic diversity for all 284 potato cultivars was 0.216, and the differentiation index of each subgroup was 0.025–0.149. Genome-wide linkage disequilibrium (LD) analysis demonstrated that the average LD was about 0.9 kb. A genome-wide association study using a mixed linear model identified 964 loci significantly associated with the late blight tolerance trait. Fourteen candidate genes for late blight tolerance traits were identified, including genes encoding late blight tolerance protein, chitinase 1, cytosolic nucleotide-binding site–leucine-rich repeat tolerance protein, protein kinase, ethylene-responsive transcription factor, and other potential plant tolerance-related proteins. This study provides novel insights into the genetic architecture of late blight tolerance traits and will be helpful for late blight tolerance in potato breeding.

scholarly journals In vitro Regeneration and Agrobacterium-mediated Transformation of Potato (Solanum tuberosum L.) Cultivars Grown in Mexico

2013 ◽  
Vol 22 (2) ◽  
pp. 93-105 ◽  
Author(s):  
Rose Onamu ◽  
Juan P Legaria ◽  
Jaime C Sahagún ◽  
José L Rodríguez ◽  
Joel N Pérez
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
In Vitro Regeneration ◽  
Potato Cultivars ◽  
Pcr Analysis ◽  
Nptii Gene ◽  
Histochemical Assay ◽  
Gus Histochemical Assay ◽  
Binary Plasmid

Prior to Agrobacterium-mediated genetic transformation in vitro regeneration protocol was established for three potato cultivars (Alfa, Cambray Rosa Morelos and Atlantic) grown in Mexico using leaf, node and internodal explants. Regeneration protocol was developed with or without the intervention of callus. Two potato cultivars, namely, Cambray Rosa Morelos and Alpha were transformed using Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121 containing the GUS and nptII genes. GUS histochemical assay and PCR analysis were conducted on rooted shoots grown in media without hormones but supplemented with antibiotics. Transformed shoots tested positive through GUS histochemical assay and integration of nptII gene was confirmed by PCR analysis DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14193 Plant Tissue Cult. & Biotech. 22(2): 93-105, 2012 (December)

Suitability of five different potato cultivars (Solanum tuberosum L.) to be processed as fresh-cut products

2009 ◽  
Vol 53 (3) ◽  
pp. 138-144 ◽  
Author(s):  
Ana B. Cabezas-Serrano ◽  
Maria L. Amodio ◽  
Rosaria Cornacchia ◽  
Raffaella Rinaldi ◽  
Giancarlo Colelli
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Potato Cultivars ◽  
Fresh Cut

scholarly journals Marcadores moleculares asociados a genes de resistencia para Globodera sp. y virus PVY en papa (Solanum tuberosum L.)

2019 ◽  
Vol 22 (2) ◽  
pp. 10-32
Author(s):  
J. Gabriel, A. Terán, S. Veramendi, M. Baldelomar, G. Main
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Globodera Rostochiensis

Las enfermedades causadas por virus y nematodos reducen los rendimientos y la calidad del tubérculo semilla en los cultivos de papa de todo el mundo, por lo que se toman diversas medidas y esfuerzos en investigación para combatir estas enfermedades. Esta investigación se planteó con el objetivo de validar tres marcadores moleculares asociados a genes de resistencia al nematodo quiste (Globodera rostochiensis y G. pallida) y al virus PVY en cultivares mejorados de papa. El estudio se realizó en condiciones de laboratorio e invernadero. En laboratorio la PCR permitió evaluar la presencia de los genes de resistencia a través del marcador RySC3 para resistencia al virus PVY y los marcadores HC y Gro 1-4 para resistencia a G. pallida y G. rostochiensis respectivamente. En invernadero el análisis de resistencia y/o susceptibilidad se determinó a través de la absorbancia (método DASELISA) y severidad para el virus PVY y la tasa de multiplicación en raíz (TMR) para el nematodo del quiste. Se verificó que el alelo para el marcador RySC3 está co-localizado con el gen Ryadg para resistencia a PVY, que fue observado en todos los cultivares a excepción de Huaycha. Por otro lado el alelo para el marcador HC que co-localiza con el gen/QTL RGp5-vrnHC para resistencia a G. pallida, se observó en 14 de las 20 cultivares evaluados, es decir el 70% mostraron el alelo de resistencia y el alelo para el marcador Gro1-4 que co-localiza con el gen Gro1-4 para resistencia a G. rostochiensis se observó en 7 de los 20 cultivares, es decir el 35% de los cultivares mostraron el alelo de resistencia a G. rostochiensis.

scholarly journals The Golden Potato Cyst Nematode Globodera rostochiensis Pathotype Ro1 in the Saint-Amable Regulated Area in Quebec, Canada

Plant Disease ◽  
2010 ◽  
Vol 94 (12) ◽  
pp. 1510-1510 ◽  
Author(s):  
A. Mahran ◽  
S. Turner ◽  
T. Martin ◽  
Q. Yu ◽  
S. Miller ◽  
...  
Keyword(s):  
Solanum Tuberosum L ◽  
Management Strategies ◽  
Solanum Species ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Potato Cultivars ◽  
Cyst Nematodes ◽  
Wild Solanum Species ◽  
Randomized Design

The golden potato cyst nematode, Globodera rostochiensis (Wollenweber, 1923) Behren, is an economically important parasite of potato (Solanum tuberosum L. subsp. tuberosum) and is recognized as a quarantine pest internationally (2). This pest attacks potato plants and causes stunting of the haulm and the root system, leaf yellowing, and tuber yield losses (2). The pathotype scheme proposed by Kort et al. distinguishes five pathotypes (Ro1, Ro2, Ro3, Ro4, and Ro5) of G. rostochiensis using differential Solanum clones (1). Several resistance genes and quantitative trait loci (QTL) that have been identified in wild Solanum species confer resistance to different G. rostochiensis pathotypes and have been introgressed into commercial potato cultivars (2). Determining G. rostochiensis pathotype(s) is essential to implement efficient management strategies, which include using resistant potato varieties. G. rostochiensis was discovered in the municipality of Saint-Amable, Quebec (QC), Canada in 2006 (3) and was subsequently characterized (4). In this study, cysts were collected from 11 representative infested fields and confirmed to be G. rostochiensis (3). The pathotypes of these nematodes were assessed on seven differential clones (S. tuberosum subsp. tuberosum cv. Desiree, S. tuberosum × S. andigena cv. Maris Piper, S. kurtzianum hybrid 60.21.19, S. vernei hybrid 62.33.3, S. vernei hybrid 58.1642/4, S. vernei hybrid 65.346/19, and S. multidissectum hybrid P55/7) in 2007 and 2009. Briefly, in each 1-liter plastic pot containing 907 g (2 lbs) of soil (3:1 loam/grit v v-1), a single differential Solanum clone was planted and 15 cysts from 1 of 11 fields were inoculated. Five replicates were set up for each Solanum-nematode combination and the pots were arranged in a completely randomized design in a greenhouse at 22°C with a 16-h light period per day. Nine weeks after inoculation, cysts were extracted from each pot and counted. Reproduction factor (Rf) for each replicate was calculated (Rf = Pf/Pi; Pf is the final cyst number and Pi is the initial cyst number added to each pot). Rf values were used to categorize the differential clones into two groups, “susceptible” leading to increase in nematode numbers (Rf > 1) and “resistant” resulting in decrease in nematode numbers (Rf ≤ 1) (1). Our results in 2007 and 2009 were consistent and the pathotype of the nematodes collected from all the fields were identified as Ro1. To our knowledge, this is the first time that the pathotype of G. rostochiensis in the Saint-Amable regulated area was identified. Accordingly, potato cultivars carrying genes or QTL resistant to G. rostochiensis pathotype Ro1 can be used to reduce the nematode numbers in infested fields in the Saint-Amable area, QC. References: (1) J. Kort et al. Nematologica 23:333, 1977. (2) R. J. Marks and B. B. Brodie. Potato Cyst Nematodes: Biology, Distribution, and Control. 1st ed. CAB International, Wallingford, 1998. (3) F. Sun et al. Plant Dis. 91:908, 2007. (4) Q. Yu et al. Can. J. Plant Pathol. 32:264, 2010.

scholarly journals Occurrence of Potato Cyst Nematode, Globodera rostochiensis, on Potato in the Saint-Amable Region, Quebec, Canada

Plant Disease ◽  
2007 ◽  
Vol 91 (7) ◽  
pp. 908-908 ◽  
Author(s):  
F. Sun ◽  
S. Miller ◽  
S. Wood ◽  
M.-J. Côté
Keyword(s):  
Dna Sequences ◽  
Dna Analysis ◽  
Solanum Tuberosum L ◽  
Tail Length ◽  
Genomic Region ◽  
Globodera Rostochiensis ◽  
Soil Survey ◽  
Globodera Pallida ◽  
Morphological Observation ◽  
Cyst Nematodes

Potato (Solanum tuberosum L. subsp. tuberosum) is one of the most important food crops in Canada. Potato cyst nematodes, Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 and Globodera pallida (Stone, 1973) Behrens, 1975, are considered the most economically important nematode pests of potatoes worldwide and are the subject of strict quarantine regulations in many countries including Canada. In Canada, G. rostochiensis was found in the Saanich Peninsula of Vancouver Island, British Columbia and both G. rostochiensis and G. pallida are present on the island of Newfoundland (3). During August of 2006, soil and roots of potato plants collected from a 19.2-ha field in the Saint-Amable region, Quebec, were submitted to the Nematology Laboratory, Canadian Food Inspection Agency, Ottawa. Golden, spherical-shaped cysts were found associated with the roots and were also extracted from the soil. The nematodes recovered were identified by morphological and morphometric analysis and DNA analysis. Measurements and morphological observation of 60 second-stage juveniles were as follows: body length = 427.4 ± 22.0 (395 to 495) μm; stylet length = 21.3 ± 1.2 (18 to 23) μm; distance from stylet base to dorsal esophageal gland opening = 5.5 ± 0.9 (3.5 to 7.0) μm; tail length = 46.7 ± 3.7 (36 to 58) μm; hyaline tail terminus = 21.1 ± 3.1 (13 to 27) μm; and the shape of stylet basal knobs varied from rounded to lateral flattened. Observations for 35 mature cysts were: number of cuticular ridges between anus and vulval fenestra = 21 ± 6 (12 to 31); fenestral length = 18.4 ± 3.6 (11 to 30) μm; distance from anus to the edge of fenestra = 81.1 ± 30.4 (29 to 165) μm; Granek's ratio = 4.4 ± 1.6 (1.8 to 6.0), with parallel wavy cuticular ridges between anus and vulval fenestra. These observations conformed to the published description of G. rostochiensis (2). Additionally, the identification was supported by the PCR-restriction fragment length polymorphism pattern of the ITS-1 region amplified using primers 18S (5′-TTGATTACGTCCCTGCCCTTT-3′) and rDNA1.58S (5′-ACGAGCCGAGTGATCCACCG-3′) and digested with BstU I and a PCR product specific for G. rostochiensis (1). Sequence of a 1,190-bp PCR fragment of ribosomal DNA amplified using primers rDNA1 (5′-TTGATTACGTCCCTGCCCTTT-3′) and rDNA2 (5′- TTTCACTCGCCGTTACTAAGG-3′) showed 99.0 to 100% identity with published DNA sequences of the same genomic region for G. rostochiensis (4). The sequence is available from the authors upon request. The origin of the introduction of G. rostochiensis into Saint-Amable is unknown. An intensive soil survey is underway to define the infested area, and strict quarantine measures have been taken to prevent further spread of G. rostochiensis. To our knowledge, this is the first evidence of the occurrence of G. rostochiensis in Quebec, Canada. References: (1) A. Fullaondo et al. Nematology 1:157, 1999. (2) A. M. Golden et al. Proc. Helminthol. Soc. Wash. 39:64, 1972. (3) A. R. Stone et al. Plant Dis. Rep. 61:590, 1977. (4) S. A. Subbotin et al. Nematology 2:591, 2000.

RESPONSE OF FOUR POTATO CULTIVARS TO METRIBUZIN TIME AND RATE OF APPLICATION

1979 ◽  
Vol 59 (2) ◽  
pp. 417-422 ◽  
Author(s):  
J. A. IVANY
Keyword(s):  
Adverse Effect ◽  
Solanum Tuberosum ◽  
Irish Cobbler ◽  
Prince Edward Island ◽  
Solanum Tuberosum L ◽  
Potato Cultivars

Over a 4-yr period (1974–1977) the effect of metribuzin at three rates and times of application was evaluated on four potato (Solanum tuberosum L.) cultivars commonly grown on Prince Edward Island. Metribuzin applied preemergence and early postemergence had no adverse effect on marketable and total yields of Irish Cobbler, Netted Gem, Kennebec, and Sebago cultivars. Delaying metribuzin application until late postemergence resulted in significantly reduced marketable yields of Irish Cobbler and Sebago but not Netted Gem and Kennebec when compared with preemergence and early postemergence treatment. Total yields with late postemergence treatment were significantly less than those with the preemergence treatment for all four cultivars and significantly less than early postemergence treatment with Irish Cobbler and Sebago.

scholarly journals Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns

2011 ◽  
Vol 49 (No. 5) ◽  
pp. 230-236 ◽  
Author(s):  
J. Bárta ◽  
V. Čurn ◽  
J. Diviš
Keyword(s):  
Solanum Tuberosum ◽  
Soluble Protein ◽  
Solanum Tuberosum L ◽  
Potato Cultivars ◽  
Protein Polymorphism ◽  
Cultivar Differences ◽  
Electrophoretic Patterns ◽  
Complex Patterns ◽  
Biochemical Variability ◽  
Protein Spectra

Biochemical variability between thirteen European and five Czech potato (Solanum tuberosum L.) cultivars grown in theCzechRepublic was studied by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns. It was confirmed that cultivar differences in protein polymorphism can be revealed by applied electrophoretic patterns. It was shown that the different character of protein and isozyme profiles required different approaches to their evaluation. For complex patterns such as electrophoretic soluble protein spectra, it is more convenient to use the evaluation of their absorbance profiles and for simpler profiles of isozymes the evaluation based on the presence or absence of a band in a definite position (simple matching) should be used. In spite of the complexity of tetraploid disposition of analysed cultivars, the results suggested higher similarity of profiles between relative cultivars and they also indicated the existence of higher similarity between cultivars from the same breeding firm.  

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