The Cartilaginous Tissue Regeneration on Weight Bearing and Non-weight Bearing Surfaces of the Knee

Cellular bioenergetics (CBE) plays a critical role in tissue regeneration. Physiologically, an enhanced metabolic state facilitates anabolic biosynthesis and mitosis to accelerate regeneration. However, the development of approaches to reprogram CBE, toward the treatment of substantial tissue injuries, has been limited thus far. Here, we show that induced repair in a rabbit model of weight-bearing bone defects is greatly enhanced using a bioenergetic-active material (BAM) scaffold compared to commercialized poly(lactic acid) and calcium phosphate ceramic scaffolds. This material was composed of energy-active units that can be released in a sustained degradation-mediated fashion once implanted. By establishing an intramitochondrial metabolic bypass, the internalized energy-active units significantly elevate mitochondrial membrane potential (ΔΨm) to supply increased bioenergetic levels and accelerate bone formation. The ready-to-use material developed here represents a highly efficient and easy-to-implement therapeutic approach toward tissue regeneration, with promise for bench-to-bedside translation.

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The role of allofibroblasts transplantation in cartilaginous tissue regeneration process

European Science Review ◽

10.20534/esr-17-1.2-126-129 ◽

2017 ◽

pp. 126-129

Author(s):

А. M. Khadjibaev ◽

A. B. Tilyakov ◽

B. A. Magrupov ◽

M. D. Urazmetova ◽

B. S. Ubaydullaev

Keyword(s):

Tissue Regeneration ◽

Regeneration Process ◽

Cartilaginous Tissue

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Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach

Life ◽

10.3390/life11090879 ◽

2021 ◽

Vol 11 (9) ◽

pp. 879

Author(s):

Paulo Ricardo Baggio Simeoni ◽

Rossana Baggio Simeoni ◽

Paulo André Bispo Machado Júnior ◽

Meila Bastos de Almeida ◽

Dilcele Silva Moreira Dziedzic ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Umbilical Cord ◽

Tissue Regeneration ◽

Amniotic Membrane ◽

Cultured Cells ◽

Human Umbilical Cord ◽

Clinical Approach ◽

Cartilaginous Tissue ◽

Tracheal Injury

Acellular amniotic membrane (AM) has been studied, with promising results on the reconstruction of lesioned tissues, and has become an attractive approach for tracheal repair. This study aimed to evaluate the repair of the trachea with human umbilical cord mesenchymal stem cells (hucMSCs) differentiated in chondrocytes, grown on an experimental model. Tracheal defects were induced by surgical tracheostomy in 30 New Zealand rabbits, and the acellular amniotic membrane, with or without cells, was covering the defect. The hucMSCs were isolated and cultivated with chondrogenic differentiation over the culture of 14 days, and then grown on the AM. In this study, the AM was biocompatible and hucMSCs differentiated into chondrocytes. Our results demonstrated an important role for AM with cultured cells in the promotion of immature collagen, known to produce tissue regeneration. In addition, cartilaginous tissue was found at the tracheal defects, demonstrated by immunohistology results. This study suggests that this biomaterial implantation can be an effective future therapeutic alternative for patients with tracheal injury.

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Hydrogel based cartilaginous tissue regeneration: recent insights and technologies

Biomaterials Science ◽

10.1039/c6bm00863a ◽

2017 ◽

Vol 5 (4) ◽

pp. 613-631 ◽

Cited By ~ 46

Author(s):

Yon Jin Chuah ◽

Yvonne Peck ◽

Jia En Josias Lau ◽

Hwan Tak Hee ◽

Dong-An Wang

Keyword(s):

Articular Cartilage ◽

Intervertebral Disc ◽

Tissue Regeneration ◽

Clinical Applications ◽

Exploratory Research ◽

Cartilaginous Tissue

Hydrogel based technologies has been extensively employed in both exploratory research and clinical applications to address numerous existing challenges in the regeneration of articular cartilage and intervertebral disc.

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Decellularized bone extracellular matrix in skeletal tissue engineering

Biochemical Society Transactions ◽

10.1042/bst20190079 ◽

2020 ◽

Vol 48 (3) ◽

pp. 755-764

Author(s):

Benjamin B. Rothrauff ◽

Rocky S. Tuan

Keyword(s):

Tissue Engineering ◽

Bone Regeneration ◽

Tissue Regeneration ◽

Animal Studies ◽

Tumor Resection ◽

Regenerative Capacity ◽

Skeletal Tissue ◽

Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

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192: Tubularized Urethral Replacement: What is the Maximum Distance for Normal Tissue Regeneration?

The Journal of Urology ◽

10.1016/s0022-5347(18)37454-8 ◽

2004 ◽

Vol 171 (4S) ◽

pp. 51-51

Author(s):

Roger E. De Filippo ◽

Hans G. Pohl ◽

James J. Yoo ◽

Anthony Atala

Keyword(s):

Tissue Regeneration ◽

Normal Tissue ◽

Maximum Distance

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Single Hemicerclage for Lateral Type B Malleolar Fracture - A Novel, Minimal and Reliable Osteosynthesis

Swiss Surgery ◽

10.1024/1023-9332.9.6.283 ◽

2003 ◽

Vol 9 (6) ◽

pp. 283-288

Author(s):

Maurer ◽

Stamenic ◽

Stouthandel ◽

Ackermann ◽

Gonzenbach

Keyword(s):

Ankle Joint ◽

Weight Bearing ◽

Fracture Type ◽

Type B ◽

Malleolar Fracture ◽

Metallic Wire ◽

Long Term Outcome ◽

X Ray ◽

Upper Ankle Joint ◽

Upper Ankle

Aim of study: To investigate the short- and long-term outcome of patients with isolated lateral malleolar fracture type B treated with a single hemicerclage out of metallic wire or PDS cord. Methods: Over an 8-year period 97 patients were treated with a single hemicerclage for lateral malleolar fracture type B and 89 were amenable to a follow-up after mean 39 months, including interview, clinical examination and X-ray controls. Results: The median operation time was 35 minutes (range 15-85 min). X-ray controls within the first two postoperative days revealed an anatomical restoration of the upper ankle joint in all but one patient. The complication rate was 8%: hematoma (2 patients), wound infection (2), Sudeck's dystrophy (2) and deep vein thrombosis (1). Full weight-bearing was tolerated at median 6.0 weeks (range 2-26 weeks). No secondary displacement, delayed union or consecutive arthrosis of the upper ankle joint was observed. All but one patient had restored symmetric joint mobility. Ninety-seven percent of patients were satisfied or very satisfied with the outcome. Following bone healing, hemicerclage removal was necessary in 19% of osteosyntheses with metallic wire and in none with PDS cord. Conclusion: The single hemicerclage is a novel, simple and reliable osteosynthesis technique for isolated lateral type B malleolar fractures and may be considered as an alternative to the osteosynthesis procedures currently in use.

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Force Plate Analysis of Dogs with Bilateral Hip Dysplasia Treated with a Unilateral Triple Pelvic Osteotomy: A Long-term Review of Cases

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.1055/s-0038-1632616 ◽

1998 ◽

Vol 11 (02) ◽

pp. 85-93 ◽

Cited By ~ 10

Author(s):

Joanne R. Cockshutt ◽

H. Dobson ◽

C. W. Miller ◽

D. L. Holmberg ◽

Connie L. Taves ◽

...

Keyword(s):

Gait Analysis ◽

Hip Dysplasia ◽

Weight Bearing ◽

Force Plate ◽

Case Series ◽

Pelvic Osteotomy ◽

Vertical Force ◽

Long Term Outcome ◽

Case Series Study

SummaryA retrospective case series study was done to determine the long-term outcome of operations upon dogs treated for canine hip dysplasia by means of a triple pelvic osteotomy (TPO). Twentyfour dogs with bilateral hip dysplasia, that received a unilateral TPO between January 1988 and June 1995, were re-examined at the Ontario Veterinary College. The assessment included physical, orthopedic and lameness examinations, standard blood work, pelvic radiographs and force plate gait analysis. They were compared to bilaterally dysplastic dogs that had not been treated, and also to normal dogs. Force plate data analysis demonstrated a significant increase in peak vertical force (PVF) and mean vertical force over stance (MVF) in the limb that underwent surgical correction by means of a TPO, when compared to the unoperated hip. It was determined that performing a unilateral TPO on a young dysplastic dog resulted in greater forces and weight bearing being projected through the TPO corrected limb when compared to the unoperated limb.Dogs with bilateral hip dysplasia treated with a unilateral triple pelvic osteotomy (TPO) were assessed by force plate gait analysis, radiographs and orthopedic examination. There was a significant increase in hip Norberg angles over time, although degenerative changes did progress. Limbs that had been operated upon had significantly greater peak and mean ground reaction forces than limbs that had not received an operation.

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