Dithiocarbamate spin traps for in vivo detection of nitric oxide production in mice

1995 ◽  
pp. 163-171 ◽  
Author(s):  
C.-S. Lai ◽  
A. M. Komarov
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Spin Traps ◽  
Oxide Production ◽  
In Vivo Detection

scholarly journals Nitrones: not only extraordinary spin traps, but also good nitric oxide sources in vivo

2015 ◽  
Vol 65 (4) ◽  
pp. 413-426 ◽  
Author(s):  
Mircea Dumitru Croitoru ◽  
Hermina Iulia Petkes ◽  
Ibolya Fülöp ◽  
Remus Cotârlan ◽  
Oana Elena Şerban ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Spin Trap ◽  
Nitrate Concentration ◽  
Plasma Concentrations ◽  
Hepatic Metabolism ◽  
Nitric Oxide Production ◽  
Spin Traps ◽  
Oxide Production

Abstract Free radicals are involved in the development of reperfusion injuries. Using a spin trap, the intensity of such lesions can be reduced. Nitrones (effective in vivo spin traps) were tried in this work as in vivo nitric oxide donors. Nitrite and nitrate concentration values (rabbit blood) were used as biomarkers of nitric oxide production. Most nitrones did not increase plasma concentrations of nitrite and nitrate; on the contrary, reduced plasma concentrations of these indicators were noted. However, glyoxal isopropyldinitrone, in a dose of 50 mg kg-1, was highly effective in increasing nitric oxide production. At the same time, nitrones do not react with hepatic homogenates, proving that the release of nitric oxide takes place in the tissues and is not related to hepatic metabolism. Before using nitrones in vivo, they were tested in vitro for the ability to release nitric oxide following a reaction with the hydroxyl radical.

Effects of Periodic Body Acceleration on the In Vivo Vasoactive Response to N-w-nitro–L-arginine and the In Vitro Nitric Oxide Production

2003 ◽  
Vol 31 (11) ◽  
pp. 1337-1346 ◽  
Author(s):  
Jose A. Adams ◽  
James E. Moore, Jr. ◽  
Michael R. Moreno ◽  
Jaqueline Coelho ◽  
Jorge Bassuk ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Body Acceleration ◽  
Oxide Production

scholarly journals Modulation of in vivo alloreactivity by inhibition of inducible nitric oxide synthase.

1995 ◽  
Vol 181 (1) ◽  
pp. 63-70 ◽  
Author(s):  
N K Worrall ◽  
W D Lazenby ◽  
T P Misko ◽  
T S Lin ◽  
C P Rodi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Biologically Active ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

The role of nitric oxide in the immune response to allogeneic tissue was explored in an in vivo cardiac transplant model in the rat. Nitric oxide production during organ rejection was demonstrated by elevations in systemic serum nitrite/nitrate levels and by electron paramagnetic resonance spectroscopy. Messenger RNA for the inducible nitric oxide synthase enzyme was detected in the rejecting allografted heart, but not in the nonrejecting isografted heart. The enzyme was demonstrated to be biologically active by the in vitro conversion of L-arginine to L-citrulline and was immunohistochemically localized to the infiltrating inflammatory cells. Treatment with aminoguanidine, a preferential inhibitor of the inducible nitric oxide synthase isoform, prevented the increased nitric oxide production in the transplanted organ and significantly attenuated the pathogenesis of acute rejection. Aminoguanidine treatment prolonged graft survival, improved graft contractile function, and significantly reduced the histologic grade of rejection. These results suggest an important role for nitric oxide in mediating the immune response to allogeneic tissue. Inhibition of inducible nitric oxide synthase may provide a novel therapeutic modality in the management of acute transplant rejection and of other immune-mediated processes.

scholarly journals Bioflavonoid-Induced Apoptosis and DNA Damage in Amastigotes and Promastigotes of Leishmania donovani: Deciphering the Mode of Action

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5843
Author(s):  
Shaila Mehwish ◽  
Sanjay Varikuti ◽  
Mubarak Ali Khan ◽  
Tariq Khan ◽  
Imdad Ullah Khan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Dna Damage ◽  
Leishmania Donovani ◽  
Genetic Material ◽  
Mechanisms Of Action ◽  
Pcr Analysis ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Ic50 Values

Natural products from plants contain many interesting biomolecules. Among them, quercetin (Q), gallic acid (GA), and rutin (R) all have well-reported antileishmanial activity; however, their exact mechanisms of action are still not known. The current study is a step forward towards unveil the possible modes of action of these compounds against Leishmania donovani (the causative agent of visceral leishmaniasis). The selected compounds were checked for their mechanisms of action against L. donovani using different biological assays including apoptosis and necrosis evaluation, effects on genetic material (DNA), quantitative testing of nitric oxide production, ultrastructural modification via transmission electron microscopy, and real-time PCR analysis. The results confirmed that these compounds are active against L. donovani, with IC50 values of 84.65 µg/mL, 86 µg/mL, and 98 µg/mL for Q, GA, and R, respectively. These compounds increased nitric oxide production and caused apoptosis and DNA damage, which led to changes in the treated cells’ ultrastructural behavior and finally to the death of L. donovani. These compounds also suppressed essential enzymes like trypanothione reductase and trypanothione synthetase, which are critical for leishmanial survival. The selected compounds have high antileishmanial potentials, and thus in-vivo testing and further screening are highly recommended.

Regulation of cerebellar nitric oxide production in response to prolonged in vivo hypoxia

1997 ◽  
Vol 49 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Yang Guo ◽  
Michael E. Ward ◽  
Stephan Beasjours ◽  
Masataka Mori ◽  
Sabah N.A. Hussain
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Oxide Production

Adenosine Modulates N -Methyl- d -Aspartate–Stimulated Hippocampal Nitric Oxide Production In Vivo

Stroke ◽  
1995 ◽  
Vol 26 (9) ◽  
pp. 1627-1633 ◽  
Author(s):  
Anish Bhardwaj ◽  
Frances J. Northington ◽  
Raymond C. Koehler ◽  
Theodore Stiefel ◽  
Daniel F. Hanley ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Oxide Production

Viral mutation accelerated by nitric oxide production during infection in vivo

2000 ◽  
Vol 14 (10) ◽  
pp. 1447-1454 ◽  
Author(s):  
Takaaki Akaike ◽  
Shigemoto Fujii ◽  
Atsushi Kato ◽  
Jun Yoshitake ◽  
Yoichi Miyamoto ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Viral Mutation

scholarly journals The 12-HHT/BLT2/NO Axis Is Associated to the Wound Healing and Skin Condition in Different Glycaemic States

2019 ◽  
Vol 7 (4) ◽  
pp. 65
Author(s):  
Leguina-Ruzzi ◽  
Ortiz Diban ◽  
Velarde
Keyword(s):  
Nitric Oxide ◽  
Wound Healing ◽  
Tight Junction ◽  
Skin Condition ◽  
Inos Mrna ◽  
Nitric Oxide Production ◽  
Mrna Levels ◽  
Oxide Production

Type 2 diabetes affects over 340 million people worldwide. This condition can go unnoticed and undiagnosed for years, leading to a late stage where high glycaemia produces complications such as delayed wound healing. Studies have shown that 12-HHT through BLT2, accelerates keratinocyte migration and wound healing. Additionally, evidence has shown the role of nitric oxide as a pro-regenerative mediator, which is decreased in diabetes. Our main goal was to study the association between the 12-HHT/BLT2 axis and the nitric oxide production in wound healing under different glycaemia conditions. For that purpose, we used in vivo and in vitro models. Our results show that the skin from diabetic mice showed reduced BLT2 and iNOS mRNA, TEER, 12-HHT, nitrites, and tight junction levels, accompanied by higher MMP9 mRNA levels. Furthermore, a positive correlation between BLT2 mRNA and nitrites was observed. In vitro, HaCaT-BLT2 cells showed higher nitric oxide and tight junction levels, and reduced MMP9 mRNA levels, compared to mock-keratinocytes under low and high glucose condition. The wound healing capacity was associated with higher nitric oxide production and was affected by the NOS inhibition. We suggest that the BLT2 expression improves the keratinocyte response to hyperglycaemia, associated with the production of nitric oxide.

A novel in vivo assay system for consecutive measurement of brain nitric oxide production combined with the microdialysis technique

1994 ◽  
Vol 176 (2) ◽  
pp. 165-168 ◽  
Author(s):  
Kouichi Ohta ◽  
Nobuo Araki ◽  
Mamoru Shibata ◽  
Junichi Hamada ◽  
Satoru Komatsumoto ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Assay System ◽  
Nitric Oxide Production ◽  
Consecutive Measurement ◽  
Oxide Production ◽  
In Vivo Assay ◽  
Brain Nitric Oxide

Induction of nitric oxide production in infiltrating leukocytes following in vivo irradiation of tumor-bearing mice

1999 ◽  
Vol 7 (2) ◽  
pp. 86-97 ◽  
Author(s):  
Yoram Vodovotz ◽  
Deborah Coffin ◽  
Anne Marie DeLuca ◽  
Leslie McKinney ◽  
John A. Cook ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Tumor Bearing ◽  
Oxide Production
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