Possible Antibiofilm Effect of Peptides Derived from IcaR Repressor of Staphylococcus epidermidis Responsible for Hospital-Acquired Sepsis

2014 ◽  
pp. 91-95
Author(s):  
Liliana Muñoz ◽  
Luz Mary Salazar ◽  
Stefany Botero ◽  
Jeannette Navarrete ◽  
Gladys Pinilla
Keyword(s):  
Staphylococcus Epidermidis ◽  
Hospital Acquired

Hospital-acquired bacterial meningitis in neurosurgical patients

1977 ◽  
Vol 46 (4) ◽  
pp. 494-500 ◽  
Author(s):  
Frederick J. Buckwold ◽  
Roger Hand ◽  
Robert R. Hansebout
Keyword(s):  
Staphylococcus Epidermidis ◽  
Skull Fracture ◽  
Cerebrospinal Fluid Leak ◽  
Enteric Bacteria ◽  
Gram Negative ◽  
Content Type ◽  
Etiologic Agents ◽  
Neurosurgical Patients ◽  
Fluid Leak ◽  
Hospital Acquired

✓ The authors review 23 cases of hospital-acquired meningitis occurring over a 15-year period in neurosurgical patients. Factors associated with the development of meningitis include recent craniotomy, cerebrospinal fluid leak, the presence of ventricular or lumbar drainage tubes, and skull fracture. Four cases were caused by Staphylococcus epidermidis; one of these patients died. In 19 cases, Gram-negative enteric bacteria were the etiologic agents, most commonly members of the Klebsiella-Enterobacter-Serratia group. Eleven of these patients died. The particular antibiotic or group of antibiotics used and the route of administration made no difference in the outcome of Gram-negative bacillary meningitis.

scholarly journals Nitazoxanide Inhibits Biofilm Formation by Staphylococcus epidermidis by Blocking Accumulation on Surfaces

2010 ◽  
Vol 54 (7) ◽  
pp. 2767-2774 ◽  
Author(s):  
Florence Tchouaffi-Nana ◽  
T. Eric Ballard ◽  
Christine H. Cary ◽  
Timothy L. Macdonald ◽  
Costi D. Sifri ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Indwelling Catheters ◽  
Biofilm Production ◽  
Cell Structures ◽  
Antiparasitic Drug ◽  
Zinc Salts ◽  
Microaerobic Conditions ◽  
Static Conditions ◽  
Hospital Acquired

ABSTRACT Coagulase-negative species of Staphylococcus are often associated with opportunistic hospital-acquired infections that arise from the colonization of indwelling catheters. Here we show that the antiparasitic drug nitazoxanide (NTZ) and its active metabolite, tizoxanide (TIZ), are inhibitory to the growth of Staphylococcus epidermidis and other staphylococci, including methicillin-resistant Staphylococcus aureus strains, under aerobic and microaerobic conditions (MICs, 8 to 16 μg/ml). At sub-MIC levels, NTZ and TIZ also inhibited biofilm production under static conditions by strains of S. epidermidis and Staphylococcus haemolyticus with a 50% inhibitory concentration of ∼2.5 μg/ml (8 μM). The 5-nitro group was required for biological activity, and a hydrophilic derivative of NTZ (AMIX) also inhibited biofilm formation. NTZ did not disperse the existing biofilm but did block further accumulation. Sub-MICs of NTZ had no effect on primary attachment to surfaces at either 4 or 37°C. The inhibitory action of NTZ and TIZ, but not vancomycin, on biofilm production could be reversed by the addition of zinc salts (2.5 to 40 μM) but not other metals, suggesting that NTZ might target the zinc-dependent accumulation-associated protein (Aap) that mediates accumulation on surfaces. However, neither NTZ nor TIZ formed chelation complexes with zinc salts, based on spectrophotometric and nuclear magnetic resonance analyses, and addition of excess zinc to NTZ-grown bacteria (apo-Aap) did not restore the accumulation phenotype. Our studies suggest that sub-MIC levels of NTZ may affect the assembly or function of cell structures associated with the biofilm phenotype.

scholarly journals A Multidrug-Resistant Staphylococcus epidermidis Clone (ST2) Is an Ongoing Cause of Hospital-Acquired Infection in a Western Australian Hospital

2012 ◽  
Vol 50 (6) ◽  
pp. 2147-2151 ◽  
Author(s):  
Micael Widerström ◽  
Cheryll A. McCullough ◽  
Geoffrey W. Coombs ◽  
Tor Monsen ◽  
Keryn J. Christiansen
Keyword(s):  
Staphylococcus Epidermidis ◽  
Multidrug Resistant ◽  
Hospital Acquired Infection ◽  
Hospital Acquired ◽  
Western Australian ◽  
Australian Hospital

scholarly journals A one-year survey of nosocomial bacteraemia at a Danish university hospital

1986 ◽  
Vol 97 (3) ◽  
pp. 471-478 ◽  
Author(s):  
K. Eliasen ◽  
P. B. Nielsen ◽  
F. Espersen
Keyword(s):  
Staphylococcus Epidermidis ◽  
Foreign Bodies ◽  
University Hospital ◽  
Focus Of Infection ◽  
One Year ◽  
Tract Infections ◽  
Catheter Infections ◽  
Hospital Acquired ◽  
Intravenous Catheters ◽  
Overall Mortality

SUMMARYA 1-year prospective study of nosocomial bacteraemia was performed at Hvidovre Hospital with special reference to frequency, focus of infection and prognosis. All patients were examined clinically in order to confirm the bacteraemia. In total, 98 hospital-acquired bacteraemias were observed, giving an incidence rate of 0·28%. Bacteraemia due to Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis predominated. The overall mortality was 38%; 65% of the patients with S. aureus bacteraemia died, 25% due to the bacteraemia. The most common types of infection were urinary tract infections and intravenous catheter infections. Fifty-five of the bacteraemias were caused by foreign bodies, mostly urinary catheters and intravenous catheters, and in 14 cases the focus was unknown. The patient population was severely ill patients. We conclude that nosocomial bacteraemia occurs specially in severely ill patients often preceded by indwelling urinary or intravenous catheters. The patients seldom die due to the bacteraemia, but they die with concomitant bacteraemia.

scholarly journals The Vancomycin Resistance-Associated Regulatory System VraSR Modulates Biofilm Formation of Staphylococcus epidermidis in an ica -Dependent Manner

mSphere ◽  
2021 ◽  
Author(s):  
Youcong Wu ◽  
Yuanyuan Meng ◽  
Lian Qian ◽  
Baixing Ding ◽  
Haiyan Han ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Regulatory System ◽  
Vancomycin Resistance ◽  
Dependent Manner ◽  
Content Type ◽  
Two Component ◽  
Complex Procedure ◽  
Hospital Acquired

S. epidermidis is a leading cause of hospital-acquired catheter-related infections, and its pathogenicity depends mostly on its ability to form biofilms on implants. The biofilm formation is a complex procedure that involves multiple regulating factors. Here, we show that a vancomycin resistance-associated two-component regulatory system, VraSR, plays an important role in modulating S. epidermidis biofilm formation and tolerance to stress.

scholarly journals Epidemiological and clinical features of the course of postoperative sternomediastinitis (clinical case)

2021 ◽  
Vol 16 (1) ◽  
pp. 56-73
Author(s):  
Olga Mironenko ◽  
◽  
Lidiia Soprun ◽  
Anna Tovanova ◽  
Denis Obuchov ◽  
...  
Keyword(s):  
Antibacterial Therapy ◽  
Staphylococcus Epidermidis ◽  
Clinical Case ◽  
Severe Disease ◽  
Hospital Environment ◽  
Period Increase ◽  
Relevant Today ◽  
Epidemiological Monitoring ◽  
Bed Days ◽  
Hospital Acquired

Health-care-related infections are becoming more relevant today. The difficulty and largely of diagnosis increased impact of adverse effects (postoperative period, increase of bed-days, use of irrational antibacterial therapy, use of excessive methods of disinfection) factors of the hospital environment on the human body, contributing to the development of severe postoperative complications, among them, sternomediastinitis occupies a special place in cardiac surgery. The clinical case we represent clearly demonstrates the atypical flow of sternominetite caused by the hypervirulent strain Klebsiella pneumoniae blaNDM-Type. Generally, sternomediastinitis causes Staphylococcus epidermidis, Staphylococcus haemolyticus. Every week the patient stays in the cardiothoracic ward of the hospital is associated with a 15 % increase in the risk of mediastinitis development. The data indicate the need for continuous epidemiological monitoring of the relevant microorganisms that are potential agents of nosocomial infections. Thus, adequate diagnosis, choice of rational treatment tactics and effective antibacterial therapy significantly improve the prognosis of the current of a given severe disease. Early epidemiological diagnosis will help to reduce the risk of the development and spread of hospital-acquired infections in the post-operative period.

scholarly journals The biofilm adhesion protein Aap from Staphylococcus epidermidis forms zinc-dependent amyloid fibers

2020 ◽  
Vol 295 (14) ◽  
pp. 4411-4427 ◽  
Author(s):  
Alexander E. Yarawsky ◽  
Stefanie L. Johns ◽  
Peter Schuck ◽  
Andrew B. Herr
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Fiber Formation ◽  
Amyloid Fibers ◽  
Adhesion Protein ◽  
Amyloid Fiber Formation ◽  
Necessary And Sufficient ◽  
Hospital Acquired ◽  
Amyloid Assembly ◽  
Accumulation Associated Protein

The skin-colonizing commensal bacterium Staphylococcus epidermidis is a leading cause of hospital-acquired and device-related infections. Its pathogenicity in humans is largely due to its propensity to form biofilms, surface-adherent bacterial accumulations that are remarkably resistant to chemical and physical stresses. Accumulation-associated protein (Aap) from S. epidermidis has been shown to be necessary and sufficient for mature biofilm formation and catheter infection. Aap contains up to 17 tandem B-repeat domains, capable of zinc-dependent assembly into twisted, rope-like intercellular filaments in the biofilm. Using microscopic and biophysical techniques, we show here that Aap B-repeat constructs assemble further into zinc-dependent functional amyloid fibers. We observed such amyloid fibers by confocal microscopy during both early and late stages of S. epidermidis biofilm formation, and we confirmed that extracellular fibrils from these biofilms contain Aap. Unlike what has been observed for amyloidogenic biofilm proteins from other bacteria, which typically use chaperones or initiator proteins to initiate amyloid assembly, our findings indicate that Aap from S. epidermidis requires Zn2+ as a catalyst that drives amyloid fiber formation, similar to many mammalian amyloid-forming proteins that require metals for assembly. This work provides detailed insights into S. epidermidis biofilm formation and architecture that improve our understanding of persistent staphylococcal infections.

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