Endophytic Bacteria: Role in Phosphate Solubilization

2017 ◽  
pp. 61-93 ◽  
Author(s):  
Abhishek Walia ◽  
Shiwani Guleria ◽  
Anjali Chauhan ◽  
Preeti Mehta
Keyword(s):  
Endophytic Bacteria ◽  
Phosphate Solubilization

Lab and Field Level Potential of Endophytic Bacteria in Managing the Dry Root Rot Disease in Black Gram Incited by Rhizoctonia bataticola (Taubenh) Butler 

2021 ◽  
Author(s):  
N. Kiran Kumar ◽  
P. Nagamani ◽  
K. Viswanath ◽  
L. Prasanthi
Keyword(s):  
Root Rot ◽  
Protease Activity ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Dry Weight ◽  
Black Gram ◽  
Rhizoctonia Bataticola

Background: The plant growth promotion and efficacy against phytopathogens by the endophytic bacteria are being focused now due to their ecofriendly nature. Methods: Endophytic bacteria (24 nos) isolated from the roots, stems and leaves of black gram plants collected from different locations were tested for their potential to inhibit the growth of R. bataticola under in vitro and in vivo conditions. Result: The leaf endophyte BLE 4 exhibited maximum inhibition (79.6%) of R. bataticola followed by BSE 4 (77.4%), BSE 7 (77.0%) and BLE 1 (74.0%). Among these 4 isolates tested as seed treatment and soil application, there was significant increase in dry weight (7.1 g), plant height (37.7 cm), number of branches (13.2) and number of pods (26.2) in BLE 4 treated plots. Whereas, the incidence of dry root rot and yield were insignificant. In the screening study of isolates for their phosphate solubilization potential, protease activity, siderophore and HCN production, no single isolate possessed all the properties, but siderophore production, protease activity and phosphate solubilization were found in BSE 4, BRE 3, BRE 5 and BRE 10 isolates. 

scholarly journals Characterization of Arsenic-Resistant Endophytic Bacteria From Alfalfa and Chickpea Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Hazhir Tashan ◽  
Behrouz Harighi ◽  
Jalal Rostamzadeh ◽  
Abdolbaset Azizi
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Lipase Production ◽  
Growth Potential ◽  
Sodium Arsenate ◽  
Plant Growth Promoting ◽  
Growth Promoting

The present investigation was carried out to isolate arsenic (As)-resistant endophytic bacteria from the roots of alfalfa and chickpea plants grown in arsenic-contamination soil, characterize their As tolerance ability, plant growth-promoting characteristics, and their role to induce As resistance by the plant. A total of four root endophytic bacteria were isolated from plants grown in As-contaminated soil (160–260-mg As kg−1 of soil). These isolates were studied for plant growth-promoting (PGP) characteristics through siderophore, phosphate solubilization, nitrogen fixation, protease, and lipase production, and the presence of the arsenate reductase (arsC) gene. Based on 16S rDNA sequence analysis, these isolates belong to the genera Acinetobacter, Pseudomonas, and Rahnella. All isolates were found As tolerant, of which one isolate, Pseudomonas sp. QNC1, showed the highest tolerance up to 350-mM concentration in the LB medium. All isolates exhibited phosphate solubilization activity. Siderophore production activity was shown by only Pseudomonas sp. QNC1, while nitrogen fixation activity was shown by only Rahnella sp. QNC2 isolate. Acinetobacter sp. QNA1, QNA2, and Rahnella sp. QNC2 exhibited lipase production, while only Pseudomonas sp. QNC1 was able to produce protease. The presence of the arsC gene was detected in all isolates. The effect of endophytic bacteria on biomass production of alfalfa and chickpea in five levels of arsenic concentrations (0-, 10-, 50-, 75-, and 100-mg kg−1 soil) was evaluated. The fresh and dry weights of roots of alfalfa and chickpea plants were decreased as the arsenic concentration of the soil was increased. Results indicate that the fresh and dry root weights of alfalfa and chickpea plants were significantly higher in endophytic bacteria-treated plants compared with non-treated plants. Inoculation of chickpea plants with Pseudomonas sp. QNC1 and Rahnella sp. QNC2 induced lower NPR3 gene expression in chickpea roots grown in soil with the final concentration of 100-mg kg−1 sodium arsenate compared with the non-endophyte-treated control. The same results were obtained in Acinetobacter sp. QNA2-treated alfalfa plants grown in the soil plus 50-mg kg−1 sodium arsenate. These results demonstrated that arsenic-resistant endophytic bacteria are potential candidates to enhance plant-growth promotion in As contamination soils. Characterization of bacterial endophytes with plant growth potential can help us apply them to improve plant yield under stress conditions.

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria of wild legumes growing on sandy soils of Binh Thuan province, Vietnam

2021 ◽  
Vol 10 (3) ◽  
pp. 246-254
Author(s):  
Dang Thi Ngoc Thanh ◽  
Pham Thi Thu Ly ◽  
Pham Thi Nga ◽  
Pham Van Ngot
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Sandy Soils ◽  
Legume Species ◽  
Bacterial Isolates ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

The roots of two legume species (Tephrosia purpurea and Tephrosia villosa) that grew wild on dry sandy soils of Binh Thuan province were sources for isolating plant growth-promoting endophytic bacteria. Semi-solid LGI medium was used for the isolation of nitrogen-fixing bacteria from root extracts. All bacterial isolates isolates were evaluated for their ability to solubilize calcium orthophosphate on solid NBRIP medium and their ability to produce IAA in Burk's liquid medium supplemented with 100 mg/L tryptophan. The possibilities of nitrogen fixation, phosphate solubilization and IAA synthesis were all quantitative examined by colorimetric method. Twenty-two bacterial isolates of T. purpurea and 18 isolates of T. villosa were capable of nitrogen fixation in the range of 1.94 to 2.81 mg/L NH4+, whereas only 18 isolates of T. purpurea and 16 isolates of T. villosa showed phosphate solubilization in the range of 12.30 – 48.90 mg/L P2O5, and IAA production in the range of 0.38 – 12.72 mg/L. Sixteen outstanding bacterial isolates of the two legume species were identified by MALDI-TOF technique. The results showed that 13 isolates had high similarity with five bacterial genera including Klebsiella, Cronobacter, Enterobacter, Burkholderia, and Bacillus with score values in the range of 2.070 – 2.411.

scholarly journals Bioprospecting of endophytic bacteria (Bacillus spp.) from passionfruit (Passiflora edulis Sims f. flavicarpa) for plant growth promotion

2019 ◽  
pp. 1369-1374
Author(s):  
Clayton dos Santos Silva ◽  
Jockeliny Mayara Camara dos Santos ◽  
João Manoel da Silva ◽  
Felipe Alexandre Tenório ◽  
Erica Livea Ferreira Guedes Celestino ◽  
...  
Keyword(s):  
Plant Growth ◽  
Liquid Medium ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Bacterial Isolates ◽  
Growth Promoters ◽  
Passiflora Edulis ◽  
Plant Growth Promoters ◽  
Passiflora Edulis Sims

Endophytic bacteria has been reported as plant growth promoters in various cultivated and uncultivated plants. Thus, the objective onf this study was to evaluate the potential of 21 endophytic bacterial isolated from leaves of passionfruit plants (Passiflora edulis Sims f. flavicarpa). In vitro antagonism, indole-3-acetic acid (IAA) production, gibberellins, cytokines and phosphate solubilization were also tested. In vitro antagonism was investigated using volatile metabolites detection by means of the overlapping dishes technique and direct confrontation. The production of IAA was evaluated by means of the colorimetric method with the absorbance reading of the optical density at O.D. (550nm). The phosphate solubilization was measured in a qualitative method by reading the solubilization halo diameter and the quantitative evaluation in liquid medium and reading of O.D. (450 nm). All bacterial isolates were able to inhibit the growth of Phytophthora sp. in both methods with values ranging from 50% to >90% inhibition (Skott-Knott, p ≤0 0.05). All the tested endophytic bacteria were also able to produce plant hormones. The phosphate solubilization was more than mean of the liquid medium. Thus, the studied endophytic bacterial isolates are suggested as potential plant growth promoters.

Potencial Agronômico de Bactérias Endofíticas de Echinodorus scaber Rataj (macrophyllus) em Plântulas de Soja

2018 ◽  
Vol 21 (3) ◽  
pp. 187
Author(s):  
Ricardo Douglas De Souza ◽  
Elisabeth Aparecida Furtado de Mendonça ◽  
Andressa Ricci Biz ◽  
Marcos Antônio Soares
Keyword(s):  
Calcium Phosphate ◽  
16S Rrna ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Colorimetric Method ◽  
Environmental Data ◽  
Rrna Gene ◽  
Soybean Seedlings

soja é umas das culturas mais produzidas no mundo, tendo safra na casa dos milhões de toneladas/ano. O aumento na produção dessa cultura permeia o desenvolvimento de tecnologias, que permitam conservar a produtividade e minimizar os dados ambientais oriundos de seu manejo. Uma opção é a inoculação de micro-organismos endofíticos que contribuem com o desenvolvimento dessa cultura. Objetivando averiguar o potencial de produção do fitormônio ácido indol acético (AIA) e solubilização de fosfato de cálcio por bactérias endofíticas isoladas de Echinodorus scaber e avaliar sua aplicação na promoção de crescimento de plântulas de soja, foram acessadas 61 bactérias endofíticas de E. scaber para os testes. A produção de AIA foi quantificada por método colorimétrico e a solubilização de fosfato de cálcio procedeu-se com inoculação das linhagens endofíticas, em meio de cultura especifico e, posteriormente, o índice de solubilização foi calculado. Para o teste de promoção de crescimento, sementes de soja foram microbiolizadas com linhagens de bactérias produtoras de AIA e solubilizadoras de fosfato de cálcio. Foram avaliadas a porcentagem de germinação, índice de velocidade de emergência e a massa seca das plântulas de soja. As linhagens de interesse foram identificadas pelo sequenciamento parcial do gene 16S rRNA. Os resultados evidenciaram, que todas as bactérias endofíticas de E. scaber avaliadas são capazes de produzir AIA e, grande parte delas pode solubilizar fosfato de cálcio in vitro e que algumas linhagens desses micro-organismos possuem traços de promoção de crescimento de plântulas de soja, evidenciado pelo aumento de massa seca.Palavras-chave: Ácido Indolacético. Solubilização de Fosfato. Promoção de Crescimento. Sementes de Soja.AbstractSoybean is one of the most produced crops in the world, having a harvest of millions of tons/year. The increase in the production of this culture permeates the development of technologies that allow to conserve the productivity and to minimize the environmental data arising from its handling. One option is the inoculation of endophytic microorganisms that contribute to the development of this culture. In order to investigate the production potential of indole acetic acid phytormonium (AIA) and calcium phosphate solubilization by endophytic bacteria isolated from Echinodorus scaber and to evaluate its application in the promotion of soybean seedlings growth, 61 endophytic E. scaber bacteria were tested . The AIA production was quantified by colorimetric method and the calcium phosphate solubilization was carried out with inoculation of the endophytic lines in specific culture medium and afterwards the solubilization index was calculated. For the growth promotion test, soybean seeds were microbiolized with strains of AIA producing bacteria and calcium phosphate solubilizers. The germination percentage, the emergence speed index and the dry mass of the soybean seedlings were evaluated. The lines of interest were identified by the partial sequencing of the 16S rRNA gene. The results showed that all the endophytic bacteria of E. scaber evaluated are capable of producing AIA and most of them can solubilize calcium phosphate in vitro and that some strains of these microorganisms have growth promoting traits of soybean seedlings evidenced by the increase of dry mass.Keywords: Indoleacetic Acid. Phosphate Solubilization. Growth Promotion. Soybean Seed.

scholarly journals Phosphate Solubilization by Endophytic Bacteria isolated from Oryza sativa

2017 ◽  
Vol 6 (10) ◽  
pp. 2713-2721 ◽  
Author(s):  
Madhusmita Borah ◽  
Pompi Das ◽  
Susanta Sarmah Pathak ◽  
Robin Chandra Boro ◽  
Madhumita Barooah
Keyword(s):  
Oryza Sativa ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization

scholarly journals Phosphate solubilization by endophytic bacteria isolated from banana trees

2017 ◽  
Vol 89 (4) ◽  
pp. 2945-2954 ◽  
Author(s):  
AMANDA D.M. MATOS ◽  
IZABELA C.P. GOMES ◽  
SILVIA NIETSCHE ◽  
ADELICA A. XAVIER ◽  
WELLINGTON S. GOMES ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Phosphate Solubilization

scholarly journals The potency of endophytic bacteria isolated from Ficus septica as phytoremediation promoting agent of Cr (VI) contaminated soil

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
Nita Shilfiani Rohmah Nita Shilfiani Rohmah ◽  
SUHARJONO ◽  
YOGA DWI JATMIKO ◽  
DIAN SISWANTO ◽  
IRFAN MUSTAFA
Keyword(s):  
Contaminated Soil ◽  
Contaminated Soils ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Phosphate Solubilization ◽  
Bacterial Isolate ◽  
Iaa Production ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Phosphate Solubilizing

Abstract. Rohmah NS, Suharjono, Jatmiko YD, Siswanto D, Mustafa I. 2020. The potency of endophytic bacteria isolated from Ficus septica as phytoremediation promoting agent of Cr (VI) contaminated soil. Biodiversitas 21: 1920-1927. Endophytic bacteria tolerant of chromium have the potential to reduce Cr (VI) to Cr (III) and produce compounds that can improve plant survival in Cr (VI) contaminated soils. This study was performed to isolate and identify Cr (VI)-tolerant bacteria from the endosphere of Ficus septica Burm.F growing on the tannery waste contaminated soil, Screening of bacteria was carried out based on the level of Cr (VI) tolerance. High tolerant isolates were tested for their potential of Indole Acetic Acid (IAA) and exopolysaccharide (EPS) production, phosphate solubilizing, and the presence of chromate reductase (ChR) genes. The most potential bacterial isolate was identified based on 16S rDNA sequences. The results showed that three isolates (E3, E4, and E8) were tolerant of Cr (VI) up to 900 mg/L. E8 isolates produced the highest IAA production (20.05 μg/mL), phosphate solubilization (127.78 μg/mL), and EPS production (9,70 μg/mL), and all isolates had the ChR gene. E8 isolate was found to be 100% identical to Enterobacter cloacae ATCC 13047.

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