scholarly journals Bioprospecting of endophytic bacteria (Bacillus spp.) from passionfruit (Passiflora edulis Sims f. flavicarpa) for plant growth promotion

2019 ◽  
pp. 1369-1374
Author(s):  
Clayton dos Santos Silva ◽  
Jockeliny Mayara Camara dos Santos ◽  
João Manoel da Silva ◽  
Felipe Alexandre Tenório ◽  
Erica Livea Ferreira Guedes Celestino ◽  
...  
Keyword(s):  
Plant Growth ◽  
Liquid Medium ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Bacterial Isolates ◽  
Growth Promoters ◽  
Passiflora Edulis ◽  
Plant Growth Promoters ◽  
Passiflora Edulis Sims

Endophytic bacteria has been reported as plant growth promoters in various cultivated and uncultivated plants. Thus, the objective onf this study was to evaluate the potential of 21 endophytic bacterial isolated from leaves of passionfruit plants (Passiflora edulis Sims f. flavicarpa). In vitro antagonism, indole-3-acetic acid (IAA) production, gibberellins, cytokines and phosphate solubilization were also tested. In vitro antagonism was investigated using volatile metabolites detection by means of the overlapping dishes technique and direct confrontation. The production of IAA was evaluated by means of the colorimetric method with the absorbance reading of the optical density at O.D. (550nm). The phosphate solubilization was measured in a qualitative method by reading the solubilization halo diameter and the quantitative evaluation in liquid medium and reading of O.D. (450 nm). All bacterial isolates were able to inhibit the growth of Phytophthora sp. in both methods with values ranging from 50% to >90% inhibition (Skott-Knott, p ≤0 0.05). All the tested endophytic bacteria were also able to produce plant hormones. The phosphate solubilization was more than mean of the liquid medium. Thus, the studied endophytic bacterial isolates are suggested as potential plant growth promoters.

scholarly journals Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion

2022 ◽  
Vol 8 ◽  
Author(s):  
Mohammad Imran Mir ◽  
Bee Hameeda ◽  
Humera Quadriya ◽  
B. Kiran Kumar ◽  
Noshin Ilyas ◽  
...  
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilization ◽  
Nitrogenase Activity ◽  
Hydrolytic Enzymes ◽  
Seed Vigor ◽  
Bacterial Isolates ◽  
Diazotrophic Bacteria ◽  
Pgp Traits ◽  
Rice Plants

A diverse group of rhizobacteria persists in the rhizospheric soil, on the surface of roots, or in association with rice plants. These bacteria colonize plant root systems, enhance plant growth and crop yield. Indigenous rhizobacteria are known to promote soil health, grain production quality and serve as sustainable bioinoculant. The present study was aimed to isolate, identify and characterize indigenous plant growth promoting (PGP) diazotrophic bacteria associated with the rhizosphere of rice fields from different areas of Jammu and Kashmir, India. A total of 15 bacteria were isolated and evaluated for various PGP traits, antagonistic activity against phytopathogens, production of hydrolytic enzymes and biofilm formation under in-vitro conditions. The majority of the isolated bacteria were Gram-negative. Out of 15 bacterial isolates, nine isolates produced IAA (12.24 ± 2.86 to 250.3 ± 1.15 μg/ml), 6 isolates exhibited phosphate solubilization activity (36.69 ± 1.63 to 312.4 ± 1.15 μg/ml), 7 isolates exhibited rock phosphate solubilization while 5 isolates solubilized zinc (10–18 mm), 7 isolates showed siderophore production, 8 isolates exhibited HCN production, 6 isolates exhibited aminocyclopropane-1-carboxylate (ACC) deaminase activity, 13 isolates exhibited cellulase activity, nine isolates exhibited amylase and lipase activity and six isolates exhibited chitinase activity. In addition, 5 isolates showed amplification with the nifH gene and showed a significant amount of nitrogenase activity in a range of 0.127–4.39 μmol C2H4/mg protein/h. Five isolates viz., IHK-1, IHK-3, IHK-13, IHK-15 and IHK-25 exhibited most PGP attributes and successfully limited the mycelial growth of Rhizoctonia solani and Fusarium oxysporum in-vitro. All the five bacterial isolates were identified based on morphological, biochemical and 16S rDNA gene sequencing study, as Stenotrophomonas maltophilia, Enterobacter sp., Bacillus sp., Ochrobactrum haematophilum and Pseudomonas aeruginosa. Rice plants developed from seeds inoculated with these PGP strains individually had considerably higher germination percentage, seed vigor index and total dry biomass when compared to control. These findings strongly imply that the PGP diazotrophic bacteria identified in this work could be employed as plant growth stimulators in rice.

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria of wild legumes growing on sandy soils of Binh Thuan province, Vietnam

2021 ◽  
Vol 10 (3) ◽  
pp. 246-254
Author(s):  
Dang Thi Ngoc Thanh ◽  
Pham Thi Thu Ly ◽  
Pham Thi Nga ◽  
Pham Van Ngot
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Sandy Soils ◽  
Legume Species ◽  
Bacterial Isolates ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

The roots of two legume species (Tephrosia purpurea and Tephrosia villosa) that grew wild on dry sandy soils of Binh Thuan province were sources for isolating plant growth-promoting endophytic bacteria. Semi-solid LGI medium was used for the isolation of nitrogen-fixing bacteria from root extracts. All bacterial isolates isolates were evaluated for their ability to solubilize calcium orthophosphate on solid NBRIP medium and their ability to produce IAA in Burk's liquid medium supplemented with 100 mg/L tryptophan. The possibilities of nitrogen fixation, phosphate solubilization and IAA synthesis were all quantitative examined by colorimetric method. Twenty-two bacterial isolates of T. purpurea and 18 isolates of T. villosa were capable of nitrogen fixation in the range of 1.94 to 2.81 mg/L NH4+, whereas only 18 isolates of T. purpurea and 16 isolates of T. villosa showed phosphate solubilization in the range of 12.30 – 48.90 mg/L P2O5, and IAA production in the range of 0.38 – 12.72 mg/L. Sixteen outstanding bacterial isolates of the two legume species were identified by MALDI-TOF technique. The results showed that 13 isolates had high similarity with five bacterial genera including Klebsiella, Cronobacter, Enterobacter, Burkholderia, and Bacillus with score values in the range of 2.070 – 2.411.

scholarly journals Plant growth-promoting activity in bean plants of endophytic bacteria isolated from Echeveria laui

2021 ◽  
Vol 5 (2) ◽  
pp. 65
Author(s):  
Anderson Emmer ◽  
João Arthur Dos Santos Oliveira ◽  
Andressa Domingos Polli ◽  
Julio Cesar Polonio ◽  
Leonardo Hamamura Alves ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Arid Environment ◽  
Bacterial Isolates ◽  
Phaseolus Vulgaris L ◽  
Bean Plants ◽  
Plant Growth Promoting ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting

Echeveria laui (Crassulaceae) is commonly commercialized due to its drought-tolerance capacity and to its rosette-shaped aesthetics. Since endophytes associated with plants from a dry or arid environment have scarcely been analyzed as yet, current research comprises the isolation of leaf endophytic bacteria from E. laui (one five-year-old and one two-year-old plants) investigating plant growth-promoting endophytic bacteria which may solubilize phosphate, fix nitrogen, produce exopolysaccharides/IAA and antagonize phytopathogens. Isolation by the maceration methodology provided a colonization rate of 1.98 x109 CFU g-1 for the two-year-old plant and 1.14 x 1010 CFU g-1 for the five-year-old one. All 40 isolates evaluated showed in vitro plant growth-promoting agent’s abilities, with emphasis on EG04, ELG18, and ELP06.  The capacity of the three best bacterial isolates were evaluated under greenhouse conditions in common and black bean (Phaseolus vulgaris L.) plants. Based on the sequencing of the 16S rRNA region and phylogenetic analysis, the three endophytes were identified as Pantoea sp. (ELG04 and ELG18) and Erwinia sp. (ELP06). Under greenhouse conditions, statistically significant differences were found among the plants treated with the three endophytes when compared to control plants for fresh and dry shoot, root biomass and length.

scholarly journals Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters

2014 ◽  
Vol 45 (4) ◽  
pp. 1333-1339 ◽  
Author(s):  
Julia del C. Martínez-Rodríguez ◽  
Marcela De la Mora-Amutio ◽  
Luis A. Plascencia-Correa ◽  
Esmeralda Audelo-Regalado ◽  
Francisco R. Guardado ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Agave Tequilana ◽  
Growth Promoters ◽  
Plant Growth Promoters

scholarly journals Endophytic bacteria associated with rice roots from suboptimal land as plant growth promoters

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Nur Prihatiningsih ◽  
HERU ADI DJATMIKO ◽  
PUJI LESTARI
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Incubation Period ◽  
Infection Rate ◽  
Endophytic Bacteria ◽  
Plant Protection ◽  
Growth Promoters ◽  
Rice Roots ◽  
Plant Growth Promoters ◽  
Promote Plant Growth

Abstract. Prihatiningsih N, Djatmiko HA, Lestari P. 2021. Endophytic bacteria associated with rice roots from suboptimal land as plant growth promoters. Biodiversitas 22: 432-437. Endophytic bacteria directly promoted plant growth and undirectly control the pathogens. This research was aimed to evaluate endophytic bacteria associated with rice root and their activity to promote plant growth and to control rice diseases. The study was conducted at the Laboratorium of Plant Protection and experimental farm Faculty of Agriculture Jenderal Soedirman University, from April to August 2020. The endophytic bacterial from suboptimal land were evaluated for promoting plant growth with soaking seed before seedling and spraying them at 10, 20, and 30 days after transplanting. The experiment was arranged with six replications and four treatments namely control (untreated endophytic bacteria) SM1 (endophytic bacteria isolate from Somagede); SB1 and SB3 (from Sumbang). Xanthomonas oyzae pv. oryzae was nature inoculation because the experiment location is in the endemic category of bacterial leaf blight. The variables observed were the plant growth components i.e plant height, number of tillers, number of panicles, incubation period, disease intensity, infection rate and effectiveness control. The result of this research shows that endophytic bacteria from Somagede (SM1) is the best to enhance plant height and number of tillers, and suppress disease intensity, and delay of incubation period.

scholarly journals The potential of endophytic bacteria isolated from Tagetes sp. to control Meloidogyne spp. infection on tomato plants

2021 ◽  
Vol 22 (6) ◽  
Author(s):  
Abdul Munif ◽  
Muhammad Nursalim ◽  
Ankardiansyah Pandu Pradana
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Parasitic Nematodes ◽  
Growth Promoter ◽  
Bacterial Isolates ◽  
Plant Parasitic Nematodes ◽  
Tomato Plants ◽  
Meloidogyne Spp ◽  
Plant Parasitic

Abstract. Munif A, Nursalim M, Pradana AP. 2021. The potential of endophytic bacteria isolated from Tagetes sp. to control Meloidogyne spp. infection on tomato plants. Biodiversitas 22: 3229-3236. The root-knot nematode (Meloidogyne spp.) is one of the plant-parasitic nematodes that cause economical loss in the vegetable plantations in the world. The endophytic bacteria may act as a potential biological agent to control the plant-parasitic nematodes. The aim of this study was to explore the endophytic bacteria from Tagetes sp., which had a potential to control Meloidogyne spp. and act as growth promoter for tomato plants. The endophytic bacteria were isolated from the roots and stems of Tagetes sp. and tissues were cultured on the tryptic soy agar (TSA) media. The endophytic bacterial isolates were selected using hypersensitive and blood agar tests to identify their biological safety. The selected endophytic bacteria were also assessed for growth promoter test in the tomato plants. The selected endophytic bacteria were assessed in vitro against the Meloidogyne spp. juvenile 2 and their effectiveness in suppressing the root-knot nematodes on tomato plants in greenhouse. The results showed that total of 184 endophytic bacterial isolates were successfully isolated from the roots and stem of Tagetes sp. In hypersensitive and hemolytic tests, 78 and 36 isolates showed negative responses, respectively. Of the 36 isolates tested, it was found that 14 of them were able to promote the growth of tomato seedlings. In in vitro test, 14 endophytic bacteria showed 73-93% mortality rate of Meloidogyne spp J2. The results at greenhouse showed that 3 bacterial isolates, namely, AL21, AL44, and AL53 suppress the number of root-knot nematodes up to 50-74% and improve tomato plant growth by 32%. These results indicate that endophytic bacteria isolated from Tagetes sp. have the potential to act as biocontrol agents of parasitic nematodes and a plant growth promoter.

scholarly journals Endophytic bacteria from root nodules of Ormosia macrocalyx with potential as plant growth promoters and antifungal activity

2018 ◽  
Vol 39 (6) ◽  
pp. 997-1005
Author(s):  
L. Hernández-Hernández ◽  
◽  
J.T. Coutiño-Megchun ◽  
C.I. Rincón-Molina ◽  
V.M. Ruíz-Valdiviezo ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Root Nodules ◽  
Growth Promoters ◽  
Plant Growth Promoters

scholarly journals Endophytic colonisation of perennial ryegrass by Trichoderma atroviride

2015 ◽  
Vol 68 ◽  
pp. 444-444
Author(s):  
A. Daryaei ◽  
R.E. Falloon ◽  
E.E. Jones ◽  
D.R.W. Kandula ◽  
H. Ghazalibiglar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Perennial Ryegrass ◽  
Field Experiments ◽  
Trichoderma Atroviride ◽  
Growth Promoters ◽  
Trichoderma Spp ◽  
Beneficial Effects ◽  
Reproductive Structures ◽  
Plant Growth Promoters

Trichoderma spp are widely used as biocontrol agents and plant growth promoters Endophytic colonisation of perennial ryegrass (Lolium perenne) by T atroviride strains was studied in laboratory greenhouse and field experiments Four strains of T atroviride were inoculated into in vitro agar cultures or potting mix with ryegrass seeds for colonisation studies The strains were also produced as prill or granule formulations for application in the field experiment Microscopy was used to observe fungal structures in plant tissue segments from pot or fieldgrown plants Segments were also surface sterilised and placed onto Trichoderma selective media Fungal colonies recovered were verified as T atroviride by sequencing the tef1 gene No endophytic colonisation occurred in any noninoculated plants and no Trichoderma endophytic colonisation was observed in fieldgrown plants However microscopy revealed fungal hyphae and reproductive structures characteristic of Trichoderma in root and stem sheath tissues of inoculated plants from in vitro cultures and pots These results were verified by sequencing the tef1 gene This study has demonstrated endophytic colonisation of ryegrass by T atroviride strains which may be related to beneficial effects on plant growth and disease control

scholarly journals AKTIVITAS METABOLIT SEKUNDER PEMACU PERTUMBUHAN DARI BAKTERI ENDOFIT ASAL KUNYIT PUTIH (Curcuma zedoaria ROSC)

2020 ◽  
Vol 31 (2) ◽  
pp. 97
Author(s):  
Tiwit Widowati ◽  
Rumella Simarmata ◽  
NFN Nuriyanah ◽  
Liseu Nurjanah ◽  
Sylvia J.R. Lekatompessy
Keyword(s):  
Plant Growth ◽  
16S Rdna ◽  
Endophytic Bacteria ◽  
Growth Promoter ◽  
Curcuma Zedoaria ◽  
Growth Promoters ◽  
Single Action ◽  
Plant Growth Promoter ◽  
Plant Growth Promoters ◽  
Medicinal Crop

<em><span>Endophytic bacteria colonize the plant tissue and contribute to the host plant's growth, development, and adaptation. This study aimed to examine the endophytic bacteria diversity associated with white turmeric (</span></em><span>Curcuma zedoaria</span><em><span>) and determine its activity as a plant growth promoter. White turmeric plants were obtained from the Indonesian Spices and Medicinal Crop Research Institute (Balittro). The isolation of endophytic bacteria from leaves, a primary and secondary rhizome of white turmeric using Nutrient Agar (NA) medium. Identification of the isolates was conducted based on 16S rDNA. The isolates were tested for their ability as a plant growth promoter and enzyme producer. Based on the morphological characteristic, 21 isolates were obtained from the primary rhizome (47.62%), secondary rhizome (19.05%), and leaves (33.33%), respectively. The sequencing result of 16S rDNA showed that the endophytic bacteria community consisted of four phyla, </span></em><span>β-proteobacteria</span><span>, γ-proteobacteria, Flavobacteria,<em> </em>Firmicutes,<em> which represented eight genera. Five isolates had several activities as a plant growth promoter, while six isolates had several enzymatic activities. Thirteen isolates had both activities, as a plant growth promoter and enzyme producer, while eight isolates only had single action. Endophytic bacteria potential as plant growth promoters can be used for supporting the cultivation of white turmeric plants.</em></span>

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