Two-Stage Culture Strategy to Enhance Both Biomass and Lipid Content of Microalgae for Biodiesel Production

Author(s):  
Redouane Benhima ◽  
Hicham El Arroussi ◽  
Abdelaziz Smouni ◽  
Najib Bendaou
2018 ◽  
Vol 118 ◽  
pp. 984-992 ◽  
Author(s):  
Lucas A. Martín ◽  
Cecilia A. Popovich ◽  
Ana M. Martínez ◽  
Paola G. Scodelaro Bilbao ◽  
María C. Damiani ◽  
...  

2019 ◽  
Vol 211 ◽  
pp. 816-822 ◽  
Author(s):  
Xia Hu ◽  
Baojun Liu ◽  
Yu Deng ◽  
Xin Bao ◽  
Aijiang Yang ◽  
...  

2021 ◽  
Vol 55 ◽  
pp. 102278
Author(s):  
Tiantao Zhao ◽  
Mengxue Liu ◽  
Tingting Zhao ◽  
Ailing Chen ◽  
Lei Zhang ◽  
...  

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Redouane Benhima ◽  
Hicham El Arroussi ◽  
Issam M. Kadmiri ◽  
Najib El Mernissi ◽  
Imane Wahby ◽  
...  

Nitrogen stress increases lipids content in microalgae, the main feedstock for algal biodiesel. Sodium tungstate was used in this study to implement nitrogen stress by inhibiting nitrate reductase (NR) in Dunaliella tertiolecta. The reduction of NR activity was accompanied by reduction of chlorophyll and accumulation of lipids. One-stage and two-stage culture strategies were compared. One-stage culture raised total lipids from 18% (control) to 39% (w: w); however, two-stage culture raised lipids to 50% in which neutral lipids were enhanced 2.14 times. To assess the quality of biodiesel produced, fatty acid methyl esters (FAME) composition was studied. It showed a slight variation of unsaturation. In addition, some physical proprieties of biodiesel were estimated and showed that higher heating values were improved by tungstate treatment. In this study, we tried to shed light on some biological impact of NR inhibition in microalgae cells using sodium tungstate which could be exploited in the improvement of biodiesel production.


2015 ◽  
Vol 80 ◽  
pp. 117-122 ◽  
Author(s):  
Chae Hun Ra ◽  
Chang-Han Kang ◽  
Na Kyoung Kim ◽  
Choul-Gyun Lee ◽  
Sung-Koo Kim

2015 ◽  
Vol 15 (2) ◽  
Author(s):  
Baiq Tri Khairina Ilhami, Lalu Japa Sri Puji Astuti dan Rina Kurnianingsih

ABSTRAK Mikroalga merupakan protista berklorofil yang memanfaatkan energi matahari dan CO2 dalam proses fotosintesis sehingga dalam biomassanya terkandung bahan-bahan seperti: lemak, protein, dan karbohidrat. Salah satu jenis mikroalga yang memiliki kandungan lemak adalah Nitzschia sp. Kelebihan mikroalga sebagai bahan baku biodiesel jika dibandingkan dengan jarak, biji bunga matahari, jagung, dan tumbuhan lainnya yaitu pertumbuhan mikroalga yang sangat cepat, tidak bersaing dengan produksi pangan, tidak membutuhkan lahan yang luas, dan ramah lingkungan. Oleh karena itu, perlu dilakukan penelitian untuk mengetahui kandungan lemak tertinggi pada Nitzschia sp. berdasarkan umur kultivasi. Dilakukan penelitian eksperimental kuantitatif menggunakan  Rancangan Acak Lengkap (RAL) dengan 5  kali panen dan 3 kali ulangan, percobaan dilakukan di Laboratorium Pakan Alami Balai Budidaya Laut (BBL) Lombok, Sekotong pada bulan Agustus sampai September 2014. Kadar lemak ditentukan dengan Metode Soxhlet. Hasil penelitian menunjukkan umur pemanenan hari ke-13 memiliki kandungan lemak tertinggi dengan persentase sebesar 1,84% sedangkan kandungan lemak terendah pada umur panen hari ke-11 dengan persentase sebesar 0,89%. Tinggi rendahnya kandungan lemak Nitzschia sp. dipengaruhi oleh nutrien, kepadatan sel dan faktor lingkungan media kultur.  ABSTRACT Photosynthetic Protist, microalgae can trap sun light energy and CO2 for photosynthesis process, and they produce lipid, protein, and carbohydrate. One species of  microalga that can produce lipid in high portion of their total dry wight is Nitzschia sp. The lipid can highly use as raw material for biodiesel production. The benefits of  microalga as source of biodiesel are they grow faster, they do not compete with other food soruces production, they do not need wide area, and they are invironmental friendly. A quantitative experiment research of rendom complete design was done in the Laboratorium Pakan Alami Balai Budidaya Laut (BBL) Lombok, Sekotong during the months of August to  September 2014. The research was conducted in five time harfesting of each in three repetation The lipid content was ditermined using Soxhlet method. The results showing that the highest lipid content (1.84%) of Nitzschia sp. occurred at the day of thirteen of culturing, and the lowest lipid content (0.89%) of Nitzschia sp. was in the day of eleven of culture. 


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