Preclinical In-Vivo Assessment of Tissue Engineered Vascular Grafts and Selection of Appropriate Animal Models

2019 ◽  
pp. 1-31 ◽  
Author(s):  
Helga Bergmeister ◽  
Bruno K. Podesser
Keyword(s):  
Animal Models ◽  
Vascular Grafts ◽  
In Vivo Assessment ◽  
Selection Of

scholarly journals Preclinical Testing of Radiopharmaceuticals for the Detection and Characterization of Osteomyelitis: Experiences from a Porcine Model

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4221
Author(s):  
Aage Kristian Olsen Alstrup ◽  
Svend Borup Jensen ◽  
Ole Lerberg Nielsen ◽  
Lars Jødal ◽  
Pia Afzelius
Keyword(s):  
Animal Model ◽  
Animal Models ◽  
Porcine Model ◽  
Animal Experiments ◽  
Radioactive Tracers ◽  
The Individual ◽  
Selection Of

The development of new and better radioactive tracers capable of detecting and characterizing osteomyelitis is an ongoing process, mainly because available tracers lack selectivity towards osteomyelitis. An integrated part of developing new tracers is the performance of in vivo tests using appropriate animal models. The available animal models for osteomyelitis are also far from ideal. Therefore, developing improved animal osteomyelitis models is as important as developing new radioactive tracers. We recently published a review on radioactive tracers. In this review, we only present and discuss osteomyelitis models. Three ethical aspects (3R) are essential when exposing experimental animals to infections. Thus, we should perform experiments in vitro rather than in vivo (Replacement), use as few animals as possible (Reduction), and impose as little pain on the animal as possible (Refinement). The gain for humans should by far exceed the disadvantages for the individual experimental animal. To this end, the translational value of animal experiments is crucial. We therefore need a robust and well-characterized animal model to evaluate new osteomyelitis tracers to be sure that unpredicted variation in the animal model does not lead to a misinterpretation of the tracer behavior. In this review, we focus on how the development of radioactive tracers relies heavily on the selection of a reliable animal model, and we base the discussions on our own experience with a porcine model.

scholarly journals Review: Tissue Engineering of Small-Diameter Vascular Grafts and Their In Vivo Evaluation in Large Animals and Humans

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 713
Author(s):  
Shu Fang ◽  
Ditte Gry Ellman ◽  
Ditte Caroline Andersen
Keyword(s):  
Animal Models ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Large Animal ◽  
Large Animal Models ◽  
Graft Outcome ◽  
Limited Success ◽  
Large Animals

To date, a wide range of materials, from synthetic to natural or a mixture of these, has been explored, modified, and examined as small-diameter tissue-engineered vascular grafts (SD-TEVGs) for tissue regeneration either in vitro or in vivo. However, very limited success has been achieved due to mechanical failure, thrombogenicity or intimal hyperplasia, and improvements of the SD-TEVG design are thus required. Here, in vivo studies investigating novel and relative long (10 times of the inner diameter) SD-TEVGs in large animal models and humans are identified and discussed, with emphasis on graft outcome based on model- and graft-related conditions. Only a few types of synthetic polymer-based SD-TEVGs have been evaluated in large-animal models and reflect limited success. However, some polymers, such as polycaprolactone (PCL), show favorable biocompatibility and potential to be further modified and improved in the form of hybrid grafts. Natural polymer- and cell-secreted extracellular matrix (ECM)-based SD-TEVGs tested in large animals still fail due to a weak strength or thrombogenicity. Similarly, native ECM-based SD-TEVGs and in-vitro-developed hybrid SD-TEVGs that contain xenogeneic molecules or matrix seem related to a harmful graft outcome. In contrast, allogeneic native ECM-based SD-TEVGs, in-vitro-developed hybrid SD-TEVGs with allogeneic banked human cells or isolated autologous stem cells, and in-body tissue architecture (IBTA)-based SD-TEVGs seem to be promising for the future, since they are suitable in dimension, mechanical strength, biocompatibility, and availability.

scholarly journals Degenerative lumbar disc disease: in vivo data support the rationale for the selection of appropriate animal models

2020 ◽  
Vol 39 ◽  
pp. 17-48
Author(s):  
M Fusellier ◽  
◽  
J Clouet ◽  
O Gauthier ◽  
M Tryfonidou ◽  
...  
Keyword(s):  
Animal Models ◽  
Lumbar Disc ◽  
Disc Disease ◽  
Lumbar Disc Disease ◽  
Data Support ◽  
Selection Of

In vivo assessment of changes to canine airway smooth muscle volume following bronchial thermoplasty with OR-OCT

2021 ◽  
Author(s):  
David C. Adams ◽  
Jasmin A. Holz ◽  
Margit V. Szabari ◽  
Lida P. Hariri ◽  
Andrew F. Mccrossan ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Canine Model ◽  
Cross Sectional ◽  
Bronchial Thermoplasty ◽  
Selection Of Patients ◽  
In Vivo Assessment ◽  
Selection Of

The inability to assess and measure changes to the airway smooth muscle (ASM) in vivo is a major challenge to evaluating asthma and its clinical outcomes. Bronchial thermoplasty (BT) is a therapy for asthma that aims to reduce the severity of excessive bronchoconstriction by ablating ASM. While multiple long-term clinical studies of BT have produced encouraging results, the outcomes of BT treatment in practice have been variable, and questions remain regarding the selection of patients. Previously we have demonstrated an imaging platform called orientation-resolved optical coherence tomography that can assess ASM endoscopically using an imaging catheter compatible with bronchoscopy. In this work, we present results obtained from a longitudinal BT study performed using a canine model (n = 8) and with the goal of investigating the use of OR-OCT for measuring the effects of BT on ASM. We demonstrate that we are capable of accurately assessing ASM both before and in the weeks following the BT procedure using blinded matching to histological samples stained with Masson's Trichome (p < 0.0001, r2 = 0.79). Analysis of volumetric ASM distributions revealed significant decreases in ASM in treated airways (average cross-sectional ASM area: 0.245 ± 0.145 mm2 pre-BT and 0.166 ± 0.112 mm2 6 weeks following BT). These results demonstrate that OR-OCT can provide clinicians with the feedback necessary to better evaluate ASM and its response to BT, and may potentially play an important role in phenotyping asthma and predicting which patients are most likely to respond to BT treatment.

A Rat Model for the In Vivo Assessment of Biological and Tissue-Engineered Valvular and Vascular Grafts

2017 ◽  
Vol 23 (12) ◽  
pp. 982-994 ◽  
Author(s):  
Yukiharu Sugimura ◽  
Anna Kathrin Schmidt ◽  
Artur Lichtenberg ◽  
Alexander Assmann ◽  
Payam Akhyari
Keyword(s):  
Rat Model ◽  
Vascular Grafts ◽  
In Vivo Assessment

Current Updates On the In vivo Assessment of Zinc Oxide Nanoparticles Toxicity Using Animal Models

2021 ◽  
Author(s):  
Ce Lynn Chong ◽  
Chee Mun Fang ◽  
Swee Yong Pung ◽  
Chin Eng Ong ◽  
Yuh Fen Pung ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Animal Models ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
In Vivo Assessment ◽  
Nanoparticles Toxicity

In Vivo Assessment of Synovial Microcirculation and Leukocyte–Endothelial Cell Interaction in Mouse Antigen-Induced Arthritis

1999 ◽  
Vol 6 (4) ◽  
pp. 281-290 ◽  
Author(s):  
A N D R E A S VEIHELMANN ◽  
ANTHONY G U S T A V E HARRIS ◽  
F R I T Z KROMBACH ◽  
E L K E SCHÜTZE ◽  
HANS JÜRGEN REFIOR ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Interaction ◽  
Endothelial Cell Interaction ◽  
In Vivo Assessment

In vivo assessment of anti-CD34 coated ePTFE vascular prostheses

2010 ◽  
Vol 58 (S 01) ◽  
Author(s):  
W Mrowczynski ◽  
A Rungatscher ◽  
F Buchegger ◽  
JC Tille ◽  
D Mugnai ◽  
...  
Keyword(s):  
Vascular Prostheses ◽  
In Vivo Assessment
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