Onopordum acanthium L. Oncopordum sp. Asteraceae

2020 ◽  
pp. 1-6
Author(s):  
Rainer W. Bussmann ◽  
Ketevan Batsatsashvili ◽  
Zaal Kikvidze
Keyword(s):  
Onopordum Acanthium

Antineoplastic Efectiveness of Silver Nanoparticles Synthesized from Onopordum Acanthium L. extract (AgNPs-OAL) toward MDA-MB231 Breast Cancer Cells

2021 ◽  
Author(s):  
Romina Delalat ◽  
Seyed Ataollah Sadat Shandiz ◽  
Bahareh Pakpour
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Annexin V ◽  
Spherical Shape ◽  
Hek293 Cells ◽  
Apoptosis Pathway ◽  
Onopordum Acanthium

Abstract The present research was done to investigate the anticancer properties of silver nanoparticles (AgNPs) fabricated using bioactive extract of Onopordum acanthium L. (AgNPs-OAL) against breast cancer cell MDA_MB231 in vitro. The determination studies of AgNPs-OAL were confirmed by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) analysis. Interestingly, FESEM image observed the spherical shape of AgNPs-OAL with the range of 1–100 nm. As AgNP-OAL exhibited significant cytotoxicity properties on breast cancer MDA_MB231 cells with IC50 values of 66.04 μg/mL, while lowing toxicity toward normal human embryonic kidney 293 (HEK293) cells with IC50 values of 101.04 μg/mL was evaluated. Further, up-regulation of apoptotic Bax and CAD genes expressions were confirmed by quantitative real-time reverse transcription-PCR (qRT-PCR) technique results. Moreover, enhanced cell cycle population (sub-G1), annexin V/PI staining, acridine orange and ethidium bromide (AO/EB) staining, Hoescht 33258 dye, and generation of reactive oxygen species (ROS) observed in AgNP-OAL-treated MDA_MB231 cancer cells. The green-synthesized AgNP-OAL has promising anticancer efficiency that can trigger apoptosis pathway in the MDA_MB231 breast cancer cells.

An Eco-friendly Fabrication of Silver Chloride Nanoparticles (AgCLNPs) using Onopordum acanthium L extract Induces Apoptosis in Breast Cancer MDA-MB-232 Cells

2021 ◽  
Author(s):  
Seyedeh Negin Shahcheraghi ◽  
Seyed Ataollah Sadat Shandiz ◽  
Bahareh Pakpour
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Cell Lines ◽  
Reverse Transcription ◽  
Hek293 Cell ◽  
Silver Chloride ◽  
Hek293 Cells ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Onopordum Acanthium

Abstract In the current experimental work, silver chloride nanoparticles (AgClNPs) were fabricated using Onopordum acanthium L extract and their apoptotic and cytotoxicity properties on breast cancer MDA_MB232 and normal HEK293 cell lines were also evaluated. AgClNPs formation was determined by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) profile. Effect of fabricated AgClNPs on MDA_MB232 and HEK293 cells viability was performed using colorimetric MTT assay. Alterations in the mRNA expression levels of CAD and Bax genes in MDA-MB-232 cells were done using quantitative real-time reverse transcription-PCR (qRT-PCR) method. Subsequently, apoptotic properties were determined using flow cytometry and fluorescence microscopy studies. MTT results investigated that AgCLNPs have a significant dose-dependent lethal activity on MDA_MB232 compared to HEK293 cell lines. Quantitative real-time reverse transcription-PCR (qRT-PCR) results have also shown that AgCLNPs could up-regulate the apoptotic Bax and CAD gene expressions in the MDA_MB232 cells. Additionally, apoptotic assessment was performed by cell cycle analysis, annexin V/PI test, Hoescht 33258 dye, acridine orange and ethidium bromide (AO/EB) staining along with the detection of the reactive oxygen species (ROS) generation. Our results suggest that novel silver chloride nanoparticles fabricated by Onopordum acanthium L extract can display some promising cytotoxic properties through inducing apoptosis pathway.

scholarly journals Standardization of Solvent Extracts from Onopordum acanthium Fruits by GC-MS, HPLC-UV/DAD, HPLC-TQMS and 1H-NMR and Evaluation of their Inhibitory Effects on the Expression of IL-8 and E-selectin in Immortalized Endothelial Cells (HUVECtert)

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Armond Daci ◽  
Markus Gold-Binder ◽  
Davide Garzon ◽  
Alessio Patea ◽  
Giangiacomo Beretta
Keyword(s):  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Inhibitory Effects ◽  
Traditional Medicines ◽  
Inflammatory Agent ◽  
Onopordum Acanthium ◽  
Tnf Α ◽  
Study Support ◽  
Anti Inflammatory ◽  
Dose Dependent

In this work we have characterized and standardized the solvent extracts of the fruits of Onopordum acanthium, a plant widely distributed from Europe to Asia and used in different traditional medicines. Fruits were extracted with methanol (ME) and n-hexane (HE) and the extract compositions determined by GC-MS, HPLC-UV/DAD, HPLC-TQMS and 1H NMR spectroscopy. Anti-inflammatory activity (IL-8 and E-selectin, qPCR and ELISA) was investigated in HUVECtert cells stimulated with TNF-α and LPS. Arctiin and isochlorogenic acid were found in ME (87±2%, w/w, and 10.2±0.2%, w/w; 38.0±3.2 mg/gFRUITS and 3.5 ± 0.4 mg/gFRUITS) and (ii) paraffins in the HE (195.6 ± 5.6 mg/g). A dose dependent (from 15 to 40 μgME/mL corresponding to 20–75 μM arctiin) inhibition of E-selectin and of the induction of IL-8 was induced by LPS. The results of this study support the use of O. acanthium fruits in traditional medicine as an anti-inflammatory agent and for cancer prevention and treatment.

scholarly journals Chemical profile and phytotoxic action of Onopordum acanthium essential oil

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Caixia Wei ◽  
Shixing Zhou ◽  
Kai Shi ◽  
Chi Zhang ◽  
Hua Shao
Keyword(s):  
Essential Oil ◽  
Chemical Profile ◽  
Onopordum Acanthium

Interpopulation variation in germination responses of Scotch thistle, Onopordum acanthium L., to various concentrations of GA3, KNO3, and NaHCO3

2000 ◽  
Vol 78 (9) ◽  
pp. 1156-1163
Author(s):  
Mirwais M Qaderi ◽  
Paul B Cavers
Keyword(s):  
Room Temperature ◽  
Germination Percentage ◽  
The Other ◽  
Germination Inhibitors ◽  
Low Concentration ◽  
Local Populations ◽  
Onopordum Acanthium ◽  
Interpopulation Variation ◽  
Two Populations

The effects of GA3, KNO3, and NaHCO3 on germination percentages and rates were determined for cypselas (seeds) of four local populations of Scotch thistle, Onopordum acanthium L., collected during a 2-year period. Three (1996, stored cypselas) and four (1997, fresh cypselas) concentrations (0.025, 0.05, and 0.1% for both years, plus 0.0125% for 1997) of the three compounds were used. Both freshly collected cypselas and those stored for 67 days at room temperature exhibited a range of germination responses from readily germinable to strongly dormant. GA3, even at a very low concentration, caused most viable cypselas to germinate, but 5-6% of viable cypselas in two populations did not respond to any GA3 treatment. In general, dry-stored cypselas germinated faster than fresh ones. Populations with more germinability (= proportion of cypselas capable of germinating) responded faster to GA3 than those with less germinability. KNO3 usually increased germination percentages but reduced the rate of germination. When treated with KNO3, stored cypselas from the two populations with less germinability germinated more slowly than those of the other two. In general, germination percentage decreased with an increase in NaHCO3 concentration. Overall, stored cypselas that were treated with NaHCO3 germinated more slowly than those in the control. The large differences among four local populations in response to these chemicals suggest that the effects of germination stimulators and other chemicals should be observed on several populations per species.Key words: cypsela, germination promoters, germination inhibitors, local populations, Onopordum acanthium, Scotch thistle.

scholarly journals Ovule Structure of Scotch thistle Onopordum acanthium L. (Cynareae, Asteraceae)

2016 ◽  
Vol 58 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Jolanta Kolczyk ◽  
Piotr Stolarczyk ◽  
Bartosz J. Płachno
Keyword(s):  
Electron Microscopy ◽  
Calcium Oxalate ◽  
Calcium Oxalate Crystals ◽  
Polarizing Microscopy ◽  
Onopordum Acanthium ◽  
Oxalate Crystals ◽  
Transmission Electron ◽  
Mature Ovule ◽  
Vacuolated Cells ◽  
Scanning Electron

AbstractStudies concerning the ultrastructure of the periendothelial zone integumentary cells of Asteraceae species are scarce. The aim was to check whether and/or what kinds of integument modifications occur inOnopordum acanthium. Ovule structure was investigated using light microscopy, scanning electron microscopy, transmission electron microscopy and histochemistry. For visualization of calcium oxalate crystals, the polarizing microscopy was used. The periendothelial zone of integument inO. acanthiumis well developed and composed of mucilage cells near the integumentary tapetum and large, highly vacuolated cells at the chalaza and therefore they differ from other integumentary cells. The cells of this zone lack starch and protein bodies. Periendothelial zone cells do not have calcium oxalate crystals, in contrast to other integument cells. The disintegration of periendothelial zone cells was observed in a mature ovule. The general ovule structure ofO. acanthiumis similar to other members of the subfamily Carduoideae, although it is different to “Taraxacum”, “Galinsoga” and “Ratibida” ovule types.

Prevalence and impact of the crown fly, Botanophila spinosa, on its host thistle, Onopordum acanthium, in southern France

2003 ◽  
Vol 24 (2) ◽  
pp. 77-86 ◽  
Author(s):  
D.T. Briese ◽  
S. Böttcher ◽  
J. Vitou ◽  
T. Thomann
Keyword(s):  
Southern France ◽  
Onopordum Acanthium
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