Mechanical Regulation of Vascularization in Three-Dimensional Engineered Tissues

2018 ◽  
pp. 37-49
Author(s):  
Barak Zohar ◽  
Shira Landau ◽  
Shulamit Levenberg
Keyword(s):  
Three Dimensional ◽  
Engineered Tissues ◽  
Mechanical Regulation

scholarly journals Collagen scaffolds functionalized with triple-helical peptides support 3D HUVEC culture

2020 ◽  
Vol 7 (5) ◽  
pp. 471-482
Author(s):  
Jean-Daniel Malcor ◽  
Emma J Hunter ◽  
Natalia Davidenko ◽  
Daniel V Bax ◽  
Ruth Cameron ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Three Dimensional ◽  
Cell Types ◽  
Cross Linking ◽  
Collagen Scaffolds ◽  
Mechanical Stiffness ◽  
Helical Peptides ◽  
Surface Receptors ◽  
Engineered Tissues

Abstract Porous biomaterials which provide a structural and biological support for cells have immense potential in tissue engineering and cell-based therapies for tissue repair. Collagen biomaterials that can host endothelial cells represent promising tools for the vascularization of engineered tissues. Three-dimensional collagen scaffolds possessing controlled architecture and mechanical stiffness are obtained through freeze–drying of collagen suspensions, followed by chemical cross-linking which maintains their stability. However, cross-linking scaffolds renders their biological activity suboptimal for many cell types, including human umbilical vein endothelial cells (HUVECs), by inhibiting cell–collagen interactions. Here, we have improved crucial HUVEC interactions with such cross-linked collagen biomaterials by covalently coupling combinations of triple-helical peptides (THPs). These are ligands for collagen-binding cell-surface receptors (integrins or discoidin domain receptors) or secreted proteins (SPARC and von Willebrand factor). THPs enhanced HUVEC adhesion, spreading and proliferation on 2D collagen films. THPs grafted to 3D-cross-linked collagen scaffolds promoted cell survival over seven days. This study demonstrates that THP-functionalized collagen scaffolds are promising candidates for hosting endothelial cells with potential for the production of vascularized engineered tissues in regenerative medicine applications.

Advances in optical imaging of dynamic three-dimensional engineered tissues

2006 ◽  
Author(s):  
Stephen A. Boppart ◽  
Claudio Vinegoni ◽  
Wei Tan ◽  
Wei Luo ◽  
Tyler S. Ralston ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Three Dimensional ◽  
Engineered Tissues

scholarly journals Isochoric supercooled preservation and revival of human cardiac microtissues

2021 ◽  
Author(s):  
Matthew J. Powell-Palm ◽  
Verena Charwat ◽  
Berenice Charrez ◽  
Brian A Siemons ◽  
Kevin E. Healy ◽  
...  
Keyword(s):  
Pluripotent Stem Cells ◽  
Organ Preservation ◽  
Drug Exposure ◽  
Structural Integrity ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Robust Method ◽  
On Demand ◽  
Engineered Tissues ◽  
Induced Pluripotent

Low-temperature ex vivo preservation and tissue engineering based on human induced pluripotent stem cells (hiPSC) represent two of the most promising routes towards on-demand access to organs for transplantation. While these fields are often considered divergent from one another, advances in both fields present critical new opportunities for crossover. Herein we demonstrate the first-ever sub-zero centigrade preservation and revival of autonomously beating three-dimensional hiPSC-derived cardiac microtissues via isochoric supercooling, without the use of chemical cryoprotectants. We show that these tissues can cease autonomous beating during preservation and resume it after warming, that the supercooling process does not affect sarcomere structural integrity, and that the tissues maintain responsiveness to drug exposure following revival. Our work suggests both that functional three dimensional (3D) engineered tissues may provide an excellent high-content, low-risk testbed to study organ preservation in a genetically human context, and that isochoric supercooling may provide a robust method for preserving and reviving engineered tissues themselves.

Functional Imaging of Matrix Structure of Cryopreserved Engineered Tissues Using Back-Directional Gated Mesoscopic Imaging

2012 ◽  
Author(s):  
Young L. Kim ◽  
Zhengbin Xu ◽  
Altug Ozcelikkale ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Clinical Use ◽  
Matrix Structure ◽  
Non Invasive ◽  
Engineered Tissues ◽  
Cell Tissue ◽  
Matrix Interactions ◽  
Non Destructive

Successful cryopreservation of engineered tissues (ETs) can greatly advance the access and availability of cell/tissue engineering products for clinical use. One of the key challenges in cryopreserving ETs is that the functionality of ETs should be maintained throughout the preservation process. Many of the functionalities are associated with the extracellular matrix (ECM) microstructure, which in turn can be a crucial marker for the post-thaw functionality. Recent studies also reported that the ECM microstructure can be affected by freezing-induced cell-fluid-matrix interactions.1–3 Thus, it is critical to assess three-dimensional (3-D) matrix structure of cryopreserved ETs in a non-destructive, non-invasive, and rapid manner.

A Device for Long Term, In Vitro Loading of Three-Dimensional Natural and Engineered Tissues

2003 ◽  
Vol 31 (11) ◽  
pp. 1347-1356 ◽  
Author(s):  
Daniel A. Shimko ◽  
Kyle K. White ◽  
Eric A. Nauman ◽  
Kay C Dee
Keyword(s):  
Three Dimensional ◽  
Engineered Tissues

3D cell aggregate printing technology and its applications

2021 ◽  
Author(s):  
Seunggyu Jeon ◽  
Se-Hwan Lee ◽  
Saeed B. Ahmed ◽  
Jonghyeuk Han ◽  
Su-Jin Heo ◽  
...  
Keyword(s):  
High Speed ◽  
High Performance ◽  
Three Dimensional ◽  
Technological Advancement ◽  
Cell Aggregate ◽  
Printing Technology ◽  
Conventional Culture ◽  
Engineered Tissues ◽  
A Cell ◽  
And Function

Abstract Various cell aggregate culture technologies have been developed and actively applied to tissue engineering and organ-on-a-chip. However, the conventional culture technologies are labor-intensive, and their outcomes are highly user dependent. In addition, the technologies cannot be used to produce three-dimensional (3D) complex tissues. In this regard, 3D cell aggregate printing technology has attracted increased attention from many researchers owing to its 3D processability. The technology allows the fabrication of 3D freeform constructs using multiple types of cell aggregates in an automated manner. Technological advancement has resulted in the development of a printing technology with a high resolution of approximately 20 μm in 3D space. A high-speed printing technology that can print a cell aggregate in milliseconds has also been introduced. The developed aggregate printing technologies are being actively applied to produce various types of engineered tissues. Although various types of high-performance printing technologies have been developed, there are still some technical obstacles in the fabrication of engineered tissues that mimic the structure and function of native tissues. This review highlights the central importance and current technical level of 3D cell aggregate printing technology, and their applications to tissue/disease models, artificial tissues, and drug-screening platforms. The paper also discusses the remaining hurdles and future directions of the printing processes.

Multi-modality three-dimensional optical imaging of engineered tissues

2004 ◽  
Author(s):  
Wei Tan ◽  
Lester J. Fahrner ◽  
Aylin Sendemir-Urkmez ◽  
Russell D. Jamison ◽  
Deborah E. Leckband ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Three Dimensional ◽  
Engineered Tissues
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