Basic Reconstruction Algorithms for Parallel Imaging

Author(s):  
Mark A. Griswold
Author(s):  
Santosh Bhattacharyya

Three dimensional microscopic structures play an important role in the understanding of various biological and physiological phenomena. Structural details of neurons, such as the density, caliber and volumes of dendrites, are important in understanding physiological and pathological functioning of nervous systems. Even so, many of the widely used stains in biology and neurophysiology are absorbing stains, such as horseradish peroxidase (HRP), and yet most of the iterative, constrained 3D optical image reconstruction research has concentrated on fluorescence microscopy. It is clear that iterative, constrained 3D image reconstruction methodologies are needed for transmitted light brightfield (TLB) imaging as well. One of the difficulties in doing so, in the past, has been in determining the point spread function of the system.We have been developing several variations of iterative, constrained image reconstruction algorithms for TLB imaging. Some of our early testing with one of them was reported previously. These algorithms are based on a linearized model of TLB imaging.


Author(s):  
Lee H. Veneklasen

This paper discusses some of the unique aspects of a spectroscopic emission microscope now being tested in Clausthal. The instrument is designed for the direct parallel imaging of both elastic and inelastic electrons from flat surfaces. Elastic contrast modes of the familiar LEEM include large and small angle LEED, mirror microscopy, backscatter diffraction contrast (for imaging of surface structure), and phase contrast (for imaging of step dynamics)(1). Inelastic modes include topology sensitive secondary, and work function sensitive photoemission. Most important, the new instrument will also allow analytical imaging using characteristic Auger or soft X-ray emissions. The basic instrument has been described by Bauer and Telieps (2). This configuration has been redesigned to include an airlock, and a LaB6 gun, triple condensor lens, magnetic objective lens, a double focussing separator field, an imaging energy analyzer, and a real time image processor.Fig. 1 shows the new configuration. The basic beam voltage supply Vo = 20 KV, upon which separate supplies for the gun Vg, specimen Vs, lens electrode Vf, and analyzer bias Vb float. The incident energy at the sample can be varied from Vs = 0-1 KV for elastic imaging, or from Vg + Vs = (3 + Vs) KV for inelastic imaging. The image energy window Vs±V/2 may be varied without readjusting either the illumation, or imaging/analyzer optics. The diagram shows conjugate diffraction and image planes. The apertures defining incoming Humiliation and outgoing image angles are placed below the separator magnet to allow for their independent optimization. The instrument can illuminate and image 0.5-100 μm fields at 0-1 keV emission energies with an energy window down to 0.2 eV.


VASA ◽  
2009 ◽  
Vol 38 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Schubert

We describe a case of aortic coarctation at the level of the infrarenal abdominal aorta which is encountered in less than six individuals in one million. In contrast to aortic narrowing above or including the renal arteries, this seems to be a relatively benign anomaly without systemic hypertension or impaired renal function. For the first time in this type of anomaly, contrast-enhanced MR angiography (ce-MRA) on a multi-receiver channel MR system, with an 8-channel phased array coil and parallel imaging was used. Ce-MRA displayed a tortuous, narrowed aortic segment that was found to be associated with mesenteric artery stenosis and compression of the orthotopic left renal vein, also known as the nutcracker phenomenon. All major aortic branches could be depicted using 3D surface-shaded displays and subvolume maximum intensity projections (MIPs). Collateral vessels of the abdominal wall were identified using whole-volume MIPs. Since the majority of aortic malformations are diagnosed at a younger age, and many suffer from renal insufficiency, we conclude that ce-MRA will eventually place conventional DSA as the modality of choice in malformations of the abdominal aorta.


Author(s):  
Wenbing Yun ◽  
Steve Wang ◽  
David Scott ◽  
Kenneth W. Nill ◽  
Waleed S. Haddad

Abstract A high-resolution table-sized x-ray nanotomography (XRMT) tool has been constructed that shows the promise of nondestructively imaging the internal structure of a full IC stack with a spatial resolution better than 100 nm. Such a tool can be used to detect, localize, and characterize buried defects in the IC. By collecting a set of X-ray projections through the full IC (which may include tens of micrometers of silicon substrate and several layers of Cu interconnects) and applying tomographic reconstruction algorithms to these projections, a 3D volumetric reconstruction can be obtained, and analyzed for defects using 3D visualization software. XRMT is a powerful technique that will find use in failure analysis and IC process development, and may facilitate or supplant investigations using SEM, TEM, and FIB tools, which generally require destructive sample preparation and a vacuum environment.


2021 ◽  
Vol 5 (3) ◽  
pp. 83
Author(s):  
Bilgi Görkem Yazgaç ◽  
Mürvet Kırcı

In this paper, we propose a fractional differential equation (FDE)-based approach for the estimation of instantaneous frequencies for windowed signals as a part of signal reconstruction. This approach is based on modeling bandpass filter results around the peaks of a windowed signal as fractional differential equations and linking differ-integrator parameters, thereby determining the long-range dependence on estimated instantaneous frequencies. We investigated the performance of the proposed approach with two evaluation measures and compared it to a benchmark noniterative signal reconstruction method (SPSI). The comparison was provided with different overlap parameters to investigate the performance of the proposed model concerning resolution. An additional comparison was provided by applying the proposed method and benchmark method outputs to iterative signal reconstruction algorithms. The proposed FDE method received better evaluation results in high resolution for the noniterative case and comparable results with SPSI with an increasing iteration number of iterative methods, regardless of the overlap parameter.


Sign in / Sign up

Export Citation Format

Share Document