Reconstructed Skin To Create In Vitro Flexible Models Of Skin Aging: New Results And Prospects

Objective: During aging, skin undergoes structural, cellular and molecular changes, which not only alter skin mechanical properties but also biological and physiological functions. Structurally the epidermis becomes thinner, the dermal epidermal junction flattens and the extra-cellular matrix component of the dermis is disorganized and degraded. The dermis is composed of two compartments: The Reticular dermis is the deepest and thickest part while the upper layer, the papillary dermis, which is much thinner and is in close contact with epidermis, plays an important role in the structure and function of the skin. We have recently shown that the papillary dermis was preferentially affected by skin aging because the activity of fibroblasts in this region was especially altered as a function of age. The purpose of this study was to investigate the capacity of a flax extract as anti-aging component. Method: We investigated the capacity of a flax extract to stimulate or restore the activity of papillary fibroblasts from young and old donors in cultured monolayers and in reconstructed skin. Several biological markers of extracellular matrix homeostasis and mechanical properties were investigated. Results: The tested flax extract seemed to improve parameters known to change with age: I/ In monolayers after treatment the number of aged fibroblasts increased II/ In reconstructed skin the flax extract appears to positively regulate some biological activities; particularly in aged fibroblasts where the deposition of laminin 5, fibrillin 1, procollagen I were increased in the dermis and the secretion of specific soluble factors like MMP1, MMP3 and KGF were regulated to levels similar to those observed in young fibroblasts III/ Mechanical properties were improved particularly for elastics parameters (R5, R2 and R7). Conclusion: The flax extract is a promising anti-aging compound. The treatment of aged papillary fibroblasts resulted in a return to a younger-like profile for some of the studied parameters.

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Phytochemical Study and In Vitro Screening Focusing on the Anti-Aging Features of Various Plants of the Greek Flora

Antioxidants ◽

10.3390/antiox10081206 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1206

Author(s):

Aimilia D. Sklirou ◽

Maria T. Angelopoulou ◽

Aikaterini Argyropoulou ◽

Eliza Chaita ◽

Vasiliki Ioanna Boka ◽

...

Keyword(s):

Plant Species ◽

High Performance ◽

High Resolution Mass Spectrometry ◽

Ultra Performance Liquid Chromatography ◽

Skin Aging ◽

Rosa Damascena ◽

Phytochemical Study ◽

Age Related

Skin health is heavily affected by ultraviolet irradiation from the sun. In addition, senile skin is characterized by major changes in the collagen, elastin and in the hyaluronan content. Natural products (NPs) have been shown to delay cellular senescence or in vivo aging by regulating age-related signaling pathways. Moreover, NPs are a preferable source of photoprotective agents and have been proven to be useful against the undesirable skin hyperpigmentation. Greek flora harvests great plant diversity with approximately 6000 plant species, as it has a wealth of NPs. Here, we report an extensive screening among hundreds of plant species. More than 440 plant species and subspecies were selected and evaluated. The extracts were screened for their antioxidant and anti-melanogenic properties, while the most promising were further subjected to various in vitro and cell-based assays related to skin aging. In parallel, their chemical profile was analyzed with High-Performance Thin-Layer Chromatography (HPTLC) and/or Ultra-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UPLC-HRMS). A variety of extracts were identified that can be of great value for the cosmetic industry, since they combine antioxidant, photoprotective, anti-melanogenic and anti-aging properties. In particular, the methanolic extracts of Sideritis scardica and Rosa damascena could be worthy of further attention, since they showed interesting chemical profiles and promising properties against specific targets involved in skin aging.

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Anti-Skin-Aging Activity of a Standardized Extract from Panax ginseng Leaves In Vitro and In Human Volunteer

Cosmetics ◽

10.3390/cosmetics4020018 ◽

2017 ◽

Vol 4 (2) ◽

pp. 18 ◽

Cited By ~ 6

Author(s):

◽

Keyword(s):

Panax Ginseng ◽

Skin Aging ◽

Human Volunteer

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Tracing skin aging process: a mini- review of in vitro approaches

Biogerontology ◽

10.1007/s10522-021-09916-z ◽

2021 ◽

Author(s):

Sophia Letsiou

Keyword(s):

Aging Process ◽

Skin Aging

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Linear viscoelastic and microstructural properties of native male human skin and in vitro 3D reconstructed skin models

Journal of the Mechanical Behavior of Biomedical Materials ◽

10.1016/j.jmbbm.2018.11.013 ◽

2019 ◽

Vol 90 ◽

pp. 644-654 ◽

Cited By ~ 6

Author(s):

Deepika Malhotra ◽

Sharadwata Pan ◽

Lars Rüther ◽

Thomas B. Goudoulas ◽

Gerrit Schlippe ◽

...

Keyword(s):

Human Skin ◽

Microstructural Properties ◽

Reconstructed Skin ◽

Linear Viscoelastic

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Anticatabolic and Anti-Inflammatory Effects of Myricetin 3-O-β-d-Galactopyranoside in UVA-Irradiated Dermal Cells via Repression of MAPK/AP-1 and Activation of TGFβ/Smad

Molecules ◽

10.3390/molecules25061331 ◽

2020 ◽

Vol 25 (6) ◽

pp. 1331 ◽

Cited By ~ 3

Author(s):

Jung Hwan Oh ◽

Fatih Karadeniz ◽

Jung Im Lee ◽

So Young Park ◽

Youngwan Seo ◽

...

Keyword(s):

Antioxidant Properties ◽

Mapk Signaling ◽

Skin Aging ◽

In Vitro Models ◽

Dermal Fibroblasts ◽

Hacat Keratinocytes ◽

Smad Pathway ◽

Collagen Production ◽

Induced Changes

UV irradiation is one of the main causes of extrinsic skin aging. UV-mediated skin aging, also known as photoaging, causes excessive breakdown of extracellular matrix which leads skin to lose its elasticity and strength. Several phytochemicals are known to exert anti-photoaging effects via different mechanisms, partly due to their antioxidant properties. The current study has been carried out to determine the potential anti-photoaging properties of myricetin 3-O-β-d-galacto-pyranoside (M3G), a flavonol glycoside isolated from L. tetragonum, in UVA-irradiated in vitro models; HaCaT keratinocytes and human dermal fibroblasts (HDFs). UVA-induced changes in MMP-1 and collagen production have been observed in HaCaT keratinocytes and HDFs. Further, UVA-induced activation of MAPK signaling, and pro-inflammatory cytokine production have been investigated. TGFβ/Smad pathway has also been analyzed in UVA-irradiated HDFs. Treatment with M3G reversed the UVA-induced changes in MMP-1 and collagen production both in HaCaT keratinocytes and HDFs. UVA-mediated activation of p38, ERK and JNK MAPK activation was also inhibited by M3G treatment in HaCaT keratinocytes. In HDFs, M3G was able to upregulate the TGFβ/Smad pathway activation. In addition, M3G downregulated the UVA-induced pro-inflammatory cytokines in keratinocytes and HDFs. It has been suggested that the M3G has exerted potential antiphotoaging properties in vitro, by attenuating UVA-induced changes in MMP-1 and collagen production in keratinocytes and dermal fibroblasts.

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UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis

Journal of Aging Research ◽

10.1155/2021/6647773 ◽

2021 ◽

Vol 2021 ◽

pp. 1-13

Author(s):

Hervé Pageon ◽

Hélène Zucchi ◽

Sylvie Ricois ◽

Philippe Bastien ◽

Daniel Asselineau

Keyword(s):

Protein Expression ◽

Biological Effect ◽

Skin Aging ◽

Chronological Aging ◽

Advanced Glycosylation End Products ◽

Uva Irradiation ◽

Inflammatory State ◽

Aged Skin

Skin aging is the result of superimposed intrinsic (individual) and extrinsic (e.g., UV exposure or nutrition) aging. Previous works have reported a relationship between UV irradiation and glycation in the aging process, leading, for example, to modified radical species production and the appearance of AGEs (advanced glycosylation end products) in increasing quantities, particularly glycoxidation products like pentosidine. In addition, the colocalization of AGEs and elastosis has also been observed. We first investigated the combination of the glycation reaction and UVA effects on a reconstructed skin model to explain their cumulative biological effect. We found that UVA exposure combined with glycation had the ability to intensify the response for specific markers: for example, MMP1 or MMP3 mRNA, proteases involved in extracellular matrix degradation, or proinflammatory cytokine, IL1α, protein expression. Moreover, the association of glycation and UVA irradiation is believed to promote an environment that favors the onset of an elastotic-like phenomenon: mRNA coding for elastin, elastase, and tropoelastin expression is increased. Secondly, because the damaging effects of UV radiation in vivo might be more detrimental in aged skin than in young skin due to increased accumulation of pentosidine and the exacerbation of alterations related to chronological aging, we studied the biological effect of soluble pentosidine in fibroblasts grown in monolayers. We found that pentosidine induced upregulation of CXCL2, IL8, and MMP12 mRNA expression (inflammatory and elastotic markers, respectively). Tropoelastin protein expression (elastin precursor) was also increased. In conclusion, fibroblasts in monolayers cultured with soluble pentosidine and tridimensional in vitro skin constructs exposed to the combination of AGEs and UVA promote an inflammatory state and an alteration of the dermal compartment in relation to an elastosis-like environment.

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