Multiple Stress Resistance in Pressure Resistant Escherichia coli Mutants

1999 ◽  
pp. 31-34
Author(s):  
K. Hauben ◽  
T. Nyström ◽  
A. Farewell ◽  
C. Michiels
Keyword(s):  
Escherichia Coli ◽  
Stress Resistance ◽  
Multiple Stress

The effect of the rpoSam allele on gene expression and stress resistance in Escherichia coli

2014 ◽  
Vol 196 (8) ◽  
pp. 589-600 ◽  
Author(s):  
Heloisa F. Galbiati ◽  
Natalia P. Taschner ◽  
Beny Spira
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Stress Resistance

scholarly journals Disruption of mitochondrial dynamics increases stress resistance through activation of multiple stress response pathways

2020 ◽  
Vol 34 (6) ◽  
pp. 8475-8492 ◽  
Author(s):  
Emily Machiela ◽  
Thomas Liontis ◽  
Dylan J. Dues ◽  
Paige D. Rudich ◽  
Annika Traa ◽  
...  
Keyword(s):  
Stress Response ◽  
Stress Resistance ◽  
Mitochondrial Dynamics ◽  
Multiple Stress

ybjX mutation regulated avian pathogenic Escherichia coli pathogenicity though stress-resistance pathway

2019 ◽  
Vol 49 (2) ◽  
pp. 144-152 ◽  
Author(s):  
Xiangjun Song ◽  
Mingyu Qiu ◽  
Huyan Jiang ◽  
Mei Xue ◽  
Jiangan Hu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stress Resistance ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli ◽  
Resistance Pathway

scholarly journals Fitness, Stress Resistance, and Extraintestinal Virulence in Escherichia coli

2013 ◽  
Vol 81 (8) ◽  
pp. 2733-2742 ◽  
Author(s):  
Alexandre Bleibtreu ◽  
Pierre-Alexis Gros ◽  
Cédric Laouénan ◽  
Olivier Clermont ◽  
Hervé Le Nagard ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Stress Response ◽  
Mouse Model ◽  
Stress Resistance ◽  
Maximum Growth ◽  
Competitive Fitness ◽  
Content Type ◽  
General Stress Response ◽  
E Coli

ABSTRACTThe extraintestinal virulence ofEscherichia coliis dependent on numerous virulence genes. However, there is growing evidence for a role of the metabolic properties and stress responses of strains in pathogenesis. We assessed the respective roles of these factors in strain virulence by developing phenotypic assays for measuringin vitroindividual and competitive fitness and the general stress response, which we applied to 82 commensal and extraintestinal pathogenicE. colistrains previously tested in a mouse model of sepsis. Individual fitness properties, in terms of maximum growth rates in various media (Luria-Bertani broth with and without iron chelator, minimal medium supplemented with gluconate, and human urine) and competitive fitness properties, estimated as the mean relative growth rate per generation in mixed cultures with a reference fluorescentE. colistrain, were highly diverse between strains. The activity of the main general stress response regulator, RpoS, as determined by iodine staining of the colonies, H2O2resistance, andrpoSsequencing, was also highly variable. No correlation between strain fitness and stress resistance and virulence in the mouse model was found, except that the maximum growth rate in urine was higher for virulent strains. Multivariate analysis showed that the number of virulence factors was the only independent factor explaining the virulence in mice. At the species level, growth capacity and stress resistance are heterogeneous properties that do not contribute significantly to the intrinsic virulence of the strains.

RcsB plays a central role in H-NS-dependent regulation of motility and acid stress resistance in Escherichia coli

2010 ◽  
Vol 161 (5) ◽  
pp. 363-371 ◽  
Author(s):  
Evelyne Krin ◽  
Antoine Danchin ◽  
Olga Soutourina
Keyword(s):  
Escherichia Coli ◽  
Stress Resistance ◽  
Acid Stress

Library sequencing strategies for comparative analysis of stress resistance mechanisms in Escherichia coli strains

2014 ◽  
Vol 31 ◽  
pp. S86
Author(s):  
Rebecca Lennen ◽  
Ida Bonde ◽  
Anna Koza ◽  
Markus Herrgård
Keyword(s):  
Escherichia Coli ◽  
Comparative Analysis ◽  
Stress Resistance ◽  
Resistance Mechanisms

scholarly journals Proline Metabolism IncreaseskatGExpression and Oxidative Stress Resistance in Escherichia coli

2014 ◽  
Vol 197 (3) ◽  
pp. 431-440 ◽  
Author(s):  
Lu Zhang ◽  
James R. Alfano ◽  
Donald F. Becker
Keyword(s):  
Oxidative Stress ◽  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Mutant Strain ◽  
Stress Resistance ◽  
Proline Metabolism ◽  
Wild Type ◽  
Content Type ◽  
Oxidative Stress Resistance ◽  
Proline Utilization

The oxidation ofl-proline to glutamate in Gram-negative bacteria is catalyzed by the proline utilization A (PutA) flavoenzyme, which contains proline dehydrogenase (PRODH) and Δ1-pyrroline-5-carboxylate (P5C) dehydrogenase domains in a single polypeptide. Previous studies have suggested that aside from providing energy, proline metabolism influences oxidative stress resistance in different organisms. To explore this potential role and the mechanism, we characterized the oxidative stress resistance of wild-type andputAmutant strains ofEscherichia coli. Initial stress assays revealed that theputAmutant strain was significantly more sensitive to oxidative stress than the parental wild-type strain. Expression of PutA in theputAmutant strain restored oxidative stress resistance, confirming that depletion of PutA was responsible for the oxidative stress phenotype. Treatment of wild-type cells with proline significantly increased hydroperoxidase I (encoded bykatG) expression and activity. Furthermore, the ΔkatGstrain failed to respond to proline, indicating a critical role for hydroperoxidase I in the mechanism of proline protection. The global regulator OxyR activates the expression ofkatGalong with several other genes involved in oxidative stress defense. In addition tokatG, proline increased the expression ofgrxA(glutaredoxin 1) andtrxC(thioredoxin 2) of the OxyR regulon, implicating OxyR in proline protection. Proline oxidative metabolism was shown to generate hydrogen peroxide, indicating that proline increases oxidative stress tolerance inE. colivia a preadaptive effect involving endogenous hydrogen peroxide production and enhanced catalase-peroxidase activity.

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