Measurement of DNA Content of Archival Material as a Guide to Prognosis

Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.

Journal of Histochemistry & Cytochemistry ◽

10.1177/31.11.6619538 ◽

1983 ◽

Vol 31 (11) ◽

pp. 1333-1335 ◽

Cited By ~ 1487

Author(s):

D W Hedley ◽

M L Friedlander ◽

I W Taylor ◽

C A Rugg ◽

E A Musgrove

Keyword(s):

Flow Cytometry ◽

Retrospective Study ◽

Clinical Outcome ◽

Dna Content ◽

Prognostic Significance ◽

Distilled Water ◽

Archival Material ◽

Cellular Dna ◽

Pathological Material ◽

Dna Histograms

A method has been developed that allows flow cytometry to be used for measuring the cellular DNA content of paraffin-embedded human tumors. Thick (i.e., 30 micron) sections were cut from tissue blocks using a microtome and dewaxed in xylene. The sections were then rehydrated by sequentially immersing them in 100, 95, 70, and 50% ethanol before finally washing in distilled water. Single cell suspensions were then prepared by incubation in 0.5% pepsin, pH 1.5, at 37 degrees C for 30 min. The cells were counted, washed, and stained with 1 microgram/ml 4',6'-diamidino-2-phenylindole for 30 min, and DNA content was measured using an ICP 22 flow cytometer. There was a good correlation between the DNA histograms produced using this method and those obtained using unfixed tissue from the same tumor stained with ethidium bromide plus mithramycin. This method allows the retrospective study of archival material where the clinical outcome is already known, and it should, therefore, be particularly useful for determining the prognostic significance of abnormal DNA content measured by flow cytometry.

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Dna content measurement can be obtained using archival material for dna flow cytometry a comparison with cytogenetic analysis in 56 pediatric solid tumors

Cancer ◽

10.1002/1097-0142(19930915)72:6<2033::aid-cncr2820720640>3.0.co;2-j ◽

1993 ◽

Vol 72 (6) ◽

pp. 2033-2041 ◽

Cited By ~ 32

Author(s):

Lynn G. Dressler ◽

Marilyn H. Duncan ◽

Elizabeth E. Varsa ◽

Thomas S. McConnell

Keyword(s):

Flow Cytometry ◽

Solid Tumors ◽

Dna Content ◽

Cytogenetic Analysis ◽

Dna Flow Cytometry ◽

Archival Material ◽

Pediatric Solid Tumors ◽

Content Measurement

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Head Size and DNA Content in Bacteriophage T4

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094255 ◽

1972 ◽

Vol 30 ◽

pp. 200-201

Author(s):

Fred Eiserling ◽

A. H. Doermann ◽

Linde Boehner

Keyword(s):

Electron Microscopy ◽

Dna Content ◽

Bacteriophage T4 ◽

Head Size ◽

Virus Particles ◽

Altered Protein ◽

A Cell ◽

Bacterial Viruses ◽

A Site ◽

Protein Shell

The control of form or shape inheritance can be approached by studying the morphogenesis of bacterial viruses. Shape variants of bacteriophage T4 with altered protein shell (capsid) size and nucleic acid (DNA) content have been found by electron microscopy, and a mutant (E920g in gene 66) controlling head size has been described. This mutant produces short-headed particles which contain 2/3 the normal DNA content and which are non-viable when only one particle infects a cell (Fig. 1).We report here the isolation of a new mutant (191c) which also appears to be in gene 66 but at a site distinct from E920g. The most striking phenotype of the mutant is the production of about 10% of the phage yield as “giant” virus particles, from 3 to 8 times longer than normal phage (Fig. 2).

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Immunoelectron microscopic localization of elastic tissue in archival material using an improved method

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161539 ◽

1990 ◽

Vol 48 (3) ◽

pp. 794-795

Author(s):

J. C. Fanning ◽

J. F. White ◽

R. Polewski ◽

E. G. Cleary

Keyword(s):

Normal Animal ◽

Animal Tissue ◽

Elastic Tissue ◽

Human Tissues ◽

Improved Method ◽

Tissue Samples ◽

Archival Material ◽

Immuno Electron Microscopy ◽

Arteries And Veins ◽

Biochemical Examination

Elastic tissue is an important component of the walls of arteries and veins, of skin, of the lungs and in lesser amounts, of many other tissues. It is responsible for the rubber-like properties of the arteries and for the normal texture of young skin. It undergoes changes in a number of important diseases such as atherosclerosis and emphysema and on exposure of skin to sunlight.We have recently described methods for the localizationof elastic tissue components in normal animal and human tissues. In the study of developing and diseased tissues it is often not possible to obtain samples which have been optimally prepared for immuno-electron microscopy. Sometimes there is also a need to examine retrospectively samples collected some years previously. We have therefore developed modifications to our published methods to allow examination of human and animal tissue samples obtained at surgery or during post mortem which have subsequently been: 1. stored frozen at -35° or -70°C for biochemical examination; 2.

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Correlation between mitochondrial DNA content, morphokinetic parameters and embryo ploidy status

10.26226/morressier.5912d9e8d462b80292386ac8 ◽

2017 ◽

Author(s):

Asina Bayram

Keyword(s):

Mitochondrial Dna ◽

Dna Content ◽

Morphokinetic Parameters ◽

Mitochondrial Dna Content ◽

Ploidy Status

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The Antonov rebel detachments in the territory of the Balashov uyezd of the Saratov gubernia (1920–1921)

Vestnik of North-Ossetian State University ◽

10.29025/1994-7720-2020-4-45-51 ◽

2020 ◽

pp. 45-51

Author(s):

Sergei V. Lyovin

Keyword(s):

Civil War ◽

Local Population ◽

Local History ◽

Local Authorities ◽

Archival Material ◽

History Of ◽

The Way

The Civil War is one of the largest tragedies in the history of our country. One of its dramatic episodes is the rebel movement led by A.S. Antonov which took place in the Tambov gubenia in 1920–1921 and was brutally suppressed by the Bolsheviks. Its scope is evidenced by the fact that it went beyond the borders of the Tambov gubernia. Separate detachments of Antonovites from the autumn of 1920 to the summer of 1921 raided the territory of the Balashov uyezd of the neighboring Saratov gubernia. The paper attempts to consider the way the uyezd authorities fought the rebels and the way civilians treated them. On the basis of an analysis of the local archival material most of which has not yet been put into scientific circulation, periodicals and the local history literature the author comes to the following conclusion: every time the invasions of Antonov’s detachments into the territory of the Balashov uyezd were so rapid that the local authorities did not manage to organize a proper rebuff, and the peasants, for the most part, supported the rebels since they saw spokesmen and defenders of their interests in them. Only frequent requisitions of peasants’ property by Antonovites as well as the replacement of the surplus appropriation system (Prodrazvyorstka) by the tax in kind (Prodnalog) led to the fact that since the spring of 1921 the support of the rebels by the local population ceased.

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