Biological Application of Widefield Surface Plasmon Resonance Microscope to Study Cell/Surface Interactions and the Effect of TGF-β3, HCL and BSA/HCL on Cell Detachment Assay of Bone Cells Monolayer

2010 ◽  
pp. 575-583
Author(s):  
Farshid Sefat ◽  
Mansour Youseffi ◽  
Morgan Denyer
Keyword(s):  
Surface Plasmon Resonance ◽  
Cell Surface ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Bone Cells ◽  
Surface Interactions ◽  
Cell Detachment ◽  
Biological Application ◽  
Cell Surface Interactions

scholarly journals Osteosphere Model to Evaluate Cell–Surface Interactions of Implantable Biomaterials

Materials ◽  
2021 ◽  
Vol 14 (19) ◽  
pp. 5858
Author(s):  
Ana Carolina Batista Brochado ◽  
Victor Hugo de Souza ◽  
Joice Correa ◽  
Suzana Azevedo dos Anjos ◽  
Carlos Fernando de Almeida Barros Mourão ◽  
...  
Keyword(s):  
Cell Surface ◽  
3D Model ◽  
Bone Cells ◽  
Three Dimensional ◽  
Surface Interactions ◽  
Force Microscopy ◽  
Tissue Therapy ◽  
Cell Surface Interactions ◽  
Titanium Surfaces

Successful biomaterials for bone tissue therapy must present different biocompatible properties, such as the ability to stimulate the migration and proliferation of osteogenic cells on the implantable surface, to increase attachment and avoid the risks of implant movement after surgery. The present work investigates the applicability of a three-dimensional (3D) model of bone cells (osteospheres) in the evaluation of osteoconductive properties of different implant surfaces. Three different titanium surface treatments were tested: machined (MA), sandblasting and acid etching (BE), and Hydroxyapatite coating by plasma spray (PSHA). The surfaces were characterized by Scanning Electron Microscopy (SEM) and atomic force microscopy (AFM), confirming that they present very distinct roughness. After seeding the osteospheres, cell–surface interactions were studied in relation to cell proliferation, migration, and spreading. The results show that BE surfaces present higher densities of cells, leaving the aggregates towards than titanium surfaces, providing more evidence of migration. The PSHA surface presented the lowest performance in all analyses. The results indicate that the 3D model allows the focal analysis of an in vitro cell/surfaces interaction of cells and surfaces. Moreover, by demonstrating the agreement with the clinical data observed in the literature, they suggest a potential use as a predictive preclinical tool for investigating osteoconductive properties of novel biomaterials for bone therapy.

Real-Time Analysis of Ligand-Induced Cell Surface and Intracellular Reactions of Living Mast Cells Using a Surface Plasmon Resonance-Based Biosensor

2002 ◽  
Vol 302 (1) ◽  
pp. 28-37 ◽  
Author(s):  
Michihiro Hide ◽  
Tomoko Tsutsui ◽  
Hajime Sato ◽  
Tomoaki Nishimura ◽  
Kenichi Morimoto ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Mast Cells ◽  
Cell Surface ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Time Analysis ◽  
Real Time Analysis

scholarly journals Dynamic Dispersal of Surface Layer Biofilm Induced by Nanosized TiO 2 Based on Surface Plasmon Resonance and Waveguide

2018 ◽  
Vol 84 (9) ◽  
Author(s):  
Peng Zhang ◽  
Jin-Song Guo ◽  
Peng Yan ◽  
You-Peng Chen ◽  
Wei Wang ◽  
...  
Keyword(s):  
Surface Layer ◽  
Surface Plasmon Resonance ◽  
Cell Surface ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Bacterial Attachment ◽  
Short Period ◽  
Bacterial Migration ◽  
Adhesive Ability ◽  
Biofilm Dispersal

ABSTRACT Pollutant degradation is present mainly in the surface layer of biofilms, and the surface layer is the most vulnerable to impairment by toxic pollutants. In this work, the effects of nanosized TiO 2 (n-TiO 2 ) on the average thicknesses of Bacillus subtilis biofilm and on bacterial attachment on different surfaces were investigated. The binding mechanism of n-TiO 2 to the cell surface was also probed. The results revealed that n-TiO 2 caused biofilm dispersal and the thicknesses decreased by 2.0 to 2.6 μm after several hours of exposure. The attachment abilities of bacteria with extracellular polymeric substances (EPS) on hydrophilic surfaces were significantly reduced by 31% and 81% under 10 and 100 mg/liter of n-TiO 2 , respectively, whereas those of bacteria without EPS were significantly reduced by 43% and 87%, respectively. The attachment abilities of bacteria with and without EPS on hydrophobic surfaces were significantly reduced by 50% and 56%, respectively, under 100 mg/liter of n-TiO 2 . The results demonstrated that biofilm dispersal can be attributed to the changes in the cell surface structure and the reduction of microbial attachment ability. IMPORTANCE Nanoparticles can penetrate into the outer layer of biofilm in a relatively short period and can bind onto EPS and bacterial surfaces. The current work probed the effects of nanosized TiO 2 (n-TiO 2 ) on biofilm thickness, bacterial migration, and surface properties of the cell in the early stage using the surface plasmon resonance waveguide mode. The results demonstrated that n-TiO 2 decreased the adhesive ability of both cell and EPS and induced bacterial migration and biofilm detachment in several hours. The decreased adhesive ability of microbes and EPS worked against microbial aggregation, reducing the effluent quality in the biological wastewater treatment process.

Bi-cell surface plasmon resonance detection of aptamer mediated thrombin capture in serum

2011 ◽  
Vol 26 (12) ◽  
pp. 4832-4836 ◽  
Author(s):  
Rinosh Joshua Mani ◽  
Roman G. Dye ◽  
Timothy A. Snider ◽  
Shaopeng Wang ◽  
Kenneth D. Clinkenbeard
Keyword(s):  
Surface Plasmon Resonance ◽  
Cell Surface ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Resonance Detection

A method for microbial cell surface fingerprinting based on surface plasmon resonance

2007 ◽  
Vol 70 (4) ◽  
pp. 595-604 ◽  
Author(s):  
Mattias Råvik ◽  
Christian Cimander ◽  
Ulla Elofsson ◽  
Andres Veide
Keyword(s):  
Surface Plasmon Resonance ◽  
Cell Surface ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Microbial Cell

scholarly journals Investigations of Surface Interactions between PEGDA-Polymer and Drug using Surface Plasmon Resonance

2021 ◽  
Vol 7 (2) ◽  
pp. 708-710
Author(s):  
Helge Lange ◽  
Natalia Rekowska ◽  
Katharina Wulf ◽  
Niels Grabow ◽  
Thomas Eickner
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Release Kinetics ◽  
Average Molecular Weight ◽  
Surface Interactions ◽  
Resonance Spectroscopy ◽  
Surface Plasmon Resonance Spectroscopy ◽  
Dexamethasone Phosphate ◽  
Kinetics Of

Abstract For the development of combination products, the determination of the release kinetics of polymer-based drug delivery systems (DDS) is a central and often timeconsuming investigation. Classical methods are often unsuitable for large-scale screening of potential polymers for combination products. We present a rapid method based on surface plasmon resonance spectroscopy (SPR), using PEGDA700 (Poly(ethylene glycol) diacrylate, average molecular weight (Mn) 700) as an example. This method is capable of determinating the kinetics of drug association and subsequent release within minutes. The proportion of desorption and diffusion can be determined separately. Surface plasmon resonance spectroscopy (SPR) is a label-free and very sensitive optical technique, which allows for real-time observation of surface interactions. The prepared SPR chips were spin-coated with PEGDA700 and crosslinked via photoinduced polymerization. The association and dissociation kinetics of dexamethasone phosphate in swollen PEGDA700 have been studied at different concentrations. The maximum loading of the surface was also obtained by this method. The study of PEGDA presented here identified the wellestablished SPR-based spectroscopy as a potential tool in the development of combination products.

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