Alphavirus Vectors: Highly Efficient Systems for Transient Gene Expression

2000 ◽  
pp. 182-210 ◽  
Author(s):  
C. Smerdou ◽  
P. Liljeström
Keyword(s):  
Gene Expression ◽  
Transient Gene Expression ◽  
Highly Efficient

scholarly journals A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes

Plant Methods ◽  
2011 ◽  
Vol 7 (1) ◽  
pp. 30 ◽  
Author(s):  
Yang Zhang ◽  
Jianbin Su ◽  
Shan Duan ◽  
Ying Ao ◽  
Jinran Dai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Gene Expression ◽  
Highly Efficient ◽  
Green Tissue

A highly efficient maize nucellus protoplast system for transient gene expression and studying programmed cell death-related processes

2015 ◽  
Vol 34 (7) ◽  
pp. 1239-1251 ◽  
Author(s):  
Jiang Chen ◽  
Qiang Yi ◽  
Qiaoheng Song ◽  
Yong Gu ◽  
Junjie Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Transient Gene Expression ◽  
Highly Efficient

scholarly journals A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species

2020 ◽  
Vol 1 (5) ◽  
pp. 100028 ◽  
Author(s):  
Youjun Zhang ◽  
Moxian Chen ◽  
Beata Siemiatkowska ◽  
Mitchell Rey Toleco ◽  
Yue Jing ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plant Species ◽  
Transient Gene Expression ◽  
Functional Studies ◽  
Highly Efficient

scholarly journals A Rapid and Highly Efficient Method for Transient Gene Expression in Rice Plants

2020 ◽  
Vol 11 ◽  
Author(s):  
Naini Burman ◽  
Divya Chandran ◽  
Jitendra P. Khurana
Keyword(s):  
Gene Expression ◽  
Efficient Method ◽  
Transient Gene Expression ◽  
Rice Plants ◽  
Highly Efficient

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

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