Effect of ABA seed pre-treatment on the response of wheat (Tritium aesitivum L.) to salinity, with special reference to plant growth, ion relations and protein patterns

2002 ◽  
pp. 145-153
Author(s):  
J. Din ◽  
T. J. Flowers
Keyword(s):  
Plant Growth ◽  
Special Reference ◽  
Protein Patterns ◽  
Ion Relations ◽  
Pre Treatment

THE SEPARATION OF THE PROTEINS OF WHEAT BY ACRYLAMIDE GEL ELECTROPHORESIS, WITH SPECIAL REFERENCE TO MOTTLED AND UNMOTTLED WHEAT

1967 ◽  
Vol 45 (1) ◽  
pp. 5-19 ◽  
Author(s):  
J. T. Barber ◽  
H. L. Wood ◽  
F. C. Steward
Keyword(s):  
Special Reference ◽  
Gel Electrophoresis ◽  
Whole Grain ◽  
Protein Patterns ◽  
Wheat Grains

The proteins of wheat grains were studied by the technique of acrylamide gel electrophoresis. This study provided the opportunity to show the effects, on the proteins resolved by these methods, of different factors and variables. These include some items of technique, effects due to the variety to which the grains belong and to the condition known as mottling, and some correlations with the morphology of the grain and with changes that accompany germination. Albumins and globulins were extracted from whole ungerminated mottled and unmottled wheat grains (var. Festival), from embryos of mottled and unmottled grain, and from the various parts of grain germinated for 2, 4, and 6 days under defined conditions. The extracted proteins were separated on [Formula: see text] acrylamide gels at pH 8.3. Glutens were extracted from the same materials and were separated on [Formula: see text] acrylamide gels at pH 5.0; some were separated at pH 4.5 also. The albumins and globulins and glutens of the whole ungerminated grain of three varieties of mottled and unmottled wheat are described and differences due to variety and mottling are discussed. The protein patterns of whole grain, dominated by endosperm, were remarkably constant though varietal effects were seen. Albumin and globulin and gluten fractions each had characteristic patterns but the observed differences between mottled and unmottled grain were quantitative, not qualitative. Proteins of embryos differed from those of endosperm and upon germination complementary changes in endosperm and embryo (roots and shoots) proteins could be observed.

scholarly journals Assessing the potential of biochar aged by humic substances to enhance plant growth and soil biological activity

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Tereza Hammerschmiedt ◽  
Jiri Holatko ◽  
Vaclav Pecina ◽  
Dalibor Huska ◽  
Oldrich Latal ◽  
...  
Keyword(s):  
Plant Growth ◽  
Humic Substances ◽  
Microbial Biomass Carbon ◽  
C:N Ratio ◽  
Organic Amendments ◽  
Respiration Activity ◽  
Soil Microbial ◽  
Activated Biochar ◽  
Pre Treatment ◽  
The Impact

Abstract Background Soil carbon-rich organic amendments (biochar, humic substances) may improve the quality and fertility of arable soil. Their co-application can additively enhance the beneficial effect on soil. Hypothetically, the pre-treatment of biochar, by aging via soaking in a solution of commercially available humic substances, could result in synergism, which may exceed the benefit from simple co-application of both amendments to the soil. Therefore, the aim of this study was to investigate the impact of biochar, humic substances, the combination of both, and the impact of biochar aged by humic substances solution on soil microbial activities and plant growth in a short-term pot experiment with lettuce. Results The aging of biochar decreased the C:N ratio as compared to non-activated biochar. The co-application of biochar and humic substances into the soil resulted in the highest microbial biomass carbon and respiration activity. The majority of enzyme activities (β-glucosidase, arylsulfatase, N-acetyl-β-d-glucosaminidase, phosphatase) were the highest in humic substances-amended soil. The application of humic substances and biochar with humic substances seemed to stimulate microbial growth and activity followed by the competition of microflora for nutrients with plants, whereas the aged biochar behaved differently. The plants treated by aged biochar achieved the highest values of dry aboveground and root biomass of all variants. However, the assumed rapid uptake of nutrients by plants resulted in lower nutrient availability for microflora, and a decline in microbial viability. Conclusions Based on this study, the positive effect of co-applied humic substances and biochar on soil fertility, quality, and health can be concluded. The usability of biochar aging by humic solution requires further study. Graphic abstract

scholarly journals Salicylic acid pre-treatment induced physiological and biochemical changes in Solanum lycopersicum L. under salinity stress

2021 ◽  
Vol 13 (2) ◽  
pp. 10917
Author(s):  
Venu SREELAKSHMY ◽  
Gunasekar ANBARASI ◽  
Benaltraja VISHNUPRIYA
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Crop Production ◽  
Plant Biomass ◽  
Salt Water ◽  
Radical Scavenging ◽  
Agricultural Crop ◽  
Pre Treatment

Agricultural crop production around the world is adversely affected by excess salt accumulation in the soil. Plants initiate broad range of signal transduction pathways to respond any stress. Salicylic acid (SA) is an endogenous plant growth regulator that acts as a signal molecule to modulate plant response by reducing the effects of abiotic stress on plants. The main objective of this study is to examine whether exogenous salicylic acid pre-treatment may reduce the adverse effects of salt stress and enhance salt tolerance in Solanum lycopersicum. For this experiment, two weeks old seedlings were subjected to salt stress by adding salt water (100 mM NaCl) for three days with or without salicylic acid pre-treatment. After salt stress exposure plant leaves were harvested and the various measures were recorded. Results of this study exhibited that salicylic acid pre-treatment mitigates various advers effects of salt stress on plant growth by stimulating plant biomass, water relations, protein content, chlorophyll pigment, and inorganic osmolytes accumulation. Simultaneously, an increase in activity of antioxidant enzymes of SOD, CAT and POX were also triggered.  This current study suggested that pre-treating of Solanum lycopersicum with salicylic acid attenuates the depressive effect of salinity by accelerating the osmolyte accumulation and triggering activity of free radical scavenging enzymes.

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