Tuber and Tuberous Root Development

Root and tuber crops have been an important part of human nutrition since the early days of humanity, providing us with essential carbohydrates, proteins, and vitamins. Today, they are especially important in tropical and subtropical regions of the world, where they help to feed an ever-growing population. Early induction and storage organ size are important agricultural traits, as they determine yield over time. During potato tuberization, environmental and metabolic status are sensed, ensuring proper timing of tuberization mediated by phloem-mobile signals. Coordinated cellular restructuring and expansion growth, as well as controlled storage metabolism in the tuber, are executed. This review summarizes our current understanding of potato tuber development and highlights similarities and differences to important tuberous root crop species like sweetpotato and cassava. Finally, we point out knowledge gaps that need to be filled before a complete picture of storage organ development can emerge. Expected final online publication date for the Annual Review of Plant Biology, Volume 72 is May 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Global View on the Cytokinin Regulatory System in Potato

Cytokinins (CKs) were earlier shown to promote potato tuberization. Our study aimed to identify and characterize CK-related genes which constitute CK regulatory system in the core potato (Solanum tuberosum) genome. For that, CK-related genes were retrieved from the sequenced genome of the S. tuberosum doubled monoploid (DM) Phureja group, classified and compared with Arabidopsis orthologs. Analysis of selected gene expression was performed with a transcriptome database for the S. tuberosum heterozygous diploid line RH89-039-16. Genes responsible for CK signaling, biosynthesis, transport, and metabolism were categorized in an organ-specific fashion. According to this database, CK receptors StHK2/3 predominate in leaves and flowers, StHK4 in roots. Among phosphotransmitters, StHP1a expression largely predominates. Surprisingly, two pseudo-phosphotransmitters intended to suppress CK effects are hardly expressed in studied organs. Among B-type RR genes, StRR1b, StRR11, and StRR18a are actively expressed, with StRR1b expressing most uniformly in all organs and StRR11 exhibiting the highest expression in roots. By cluster analysis four types of prevailing CK-signaling chains were identified in (1) leaves and flowers, StHK2/3→StHP1a→StRR1b/+; (2) shoot apical meristems, stolons, and mature tubers, StHK2/4→StHP1a→StRR1b/+; (3) stems and young tubers, StHK2/4→StHP1a→StRR1b/11/18a; and (4) roots and tuber sprouts, StHK4→StHP1a→StRR11/18a. CK synthesis genes StIPT3/5 and StCYP735A are expressed mainly in roots followed by tuber sprouts, but rather weakly in stolons and tubers. By contrast, CK-activation genes StLOGs are active in stolons, and StLOG3b expression is even stolon-confined. Apparently, the main CK effects on tuber initiation are realized via activity of StLOG1/3a/3b/7c/8a genes in stolons. Current advances and future directions in potato research are discussed.

The Unexploited Potential of Nutrient Analysis in Potato Tissues at the Onset of Tuberization for Tuber Yield Prediction

Nutrient analysis of potato leaves in early growth is not sufficient for a reliable prediction of tuber yield. This hypothesis was verified based on a field experiment conducted during 2006–2008. The experimental factors were: nitrogen (N) rates (60, 120 kg ha−1), fertilizers (Urea, Urea + inhibitor—NBPT ([N-(n-butyl) thiophosphoric triamide]), and sulfur rates (0, 50 kg ha−1). Plant material for nutrient determination (N, P, K, Mg, Ca, Fe, Mn, Zn, Cu), which included leaves, stems, and stolons + roots (R+S), was sampled at BBCH 39/40. The marketable tuber yield (MTY) was in the ranges of 43–75, 44–70, and 24–38 t ha−1, in 2006, 2007, and 2008, respectively. The MTY and contents of N, Zn, and Cu, irrespective of the potato tissue, showed the same seasonal pattern, reaching the lowest values in the dry 2008. The N content in stems was the best tuber yield predictor. A shortage of K in stems and Mg and Cu in R+S, due to the opposite effect of Ca, reduced the N content. An N:Ca ratio in stems greater than 10:1 resulted in yield decrease. A reliable indication of nutrients limiting the tuber yield at the onset of potato tuberization requires data on the nutrient status in both leaves and stems.

COMBINED ANALYSIS OF RADARSAT-2 SAR AND SENTINEL-2 OPTICAL DATA FOR IMPROVED MONITORING OF TUBER INITIATION STAGE OF POTATO

<p><strong>Abstract.</strong> Tuber initiation and tuber bulking stages are critical part of various phenological phases for potato production. Tuber initiation covers the period from the formation of spherical rhizome ends, the flowering and the start of tuber bulking. In general, the tuberization spans from 3 to 5 weeks after emergence and ends with the row closer i.e. canopies in adjacent rows touch each other across the furrow. Hence, this rapid growth seeks critical agronomic management practices such as irrigation and fertilization. It majorly influences the growth of stems, foliar area, dry weight and number of tubers particularly at the phase of tuber initiation. During these phenological stages, potato crops are susceptible to the infestation of late blight diseases caused by <i>Phytophthora infestans</i> and largely affects the potato production. Thus identifying the crop risk using remote sensing approaches can provide an efficient potato growth monitoring framework. In the context of monitoring crop dynamics, quad-pol Synthetic Aperture Radar (SAR) data has proven to be effective due to its sensitivity towards dielectric and geometric properties. In addition to SAR data, optical remote sensing data derived vegetation information can provide an improved insight into crop growth when combined with SAR data. In this research, quad-pol RADARSAT-2 and Sentinel-2 optical data are analyzed to monitor potato tuberization phase over Bardhaman district in the state ofWest Bengal, which is one of the major potato growing regions in India. The proposed approach uses polarimetric parameters such as backscatter intensities, ratio (HH/VV, VH/VV, linear depolarization ratio), and co-pol correlation (<i>&amp;rho;_HH–VV</i>) along with the vegetation indices derived from the Sentinel-2 data for understanding the spatio-temporal dynamics. The initial results show a promising accuracy in monitoring the dynamics of potato tuberization. Integration of such earth observation (EO) data, in conjunction with in-situ field measurements, might significantly enhance the current capabilities for crop monitoring.</p>

Microarray analysis of gene expression patterns in the leaf during potato tuberization in the potato somatic hybrid Solanum tuberosum and Solanum etuberosum

Genes involved in photoassimilate partitioning and changes in hormonal balance are important for potato tuberization. In the present study, we investigated gene expression patterns in the tuber-bearing potato somatic hybrid (E1-3) and control non-tuberous wild species Solanum etuberosum (Etb) by microarray. Plants were grown under controlled conditions and leaves were collected at eight tuber developmental stages for microarray analysis. A t-test analysis identified a total of 468 genes (94 up-regulated and 374 down-regulated) that were statistically significant (p ≤ 0.05) and differentially expressed in E1-3 and Etb. Gene Ontology (GO) characterization of the 468 genes revealed that 145 were annotated and 323 were of unknown function. Further, these 145 genes were grouped based on GO biological processes followed by molecular function and (or) PGSC description into 15 gene sets, namely (1) transport, (2) metabolic process, (3) biological process, (4) photosynthesis, (5) oxidation-reduction, (6) transcription, (7) translation, (8) binding, (9) protein phosphorylation, (10) protein folding, (11) ubiquitin-dependent protein catabolic process, (12) RNA processing, (13) negative regulation of protein, (14) methylation, and (15) mitosis. RT-PCR analysis of 10 selected highly significant genes (p ≤ 0.01) confirmed the microarray results. Overall, we show that candidate genes induced in leaves of E1-3 were implicated in tuberization processes such as transport, carbohydrate metabolism, phytohormones, and transcription/translation/binding functions. Hence, our results provide an insight into the candidate genes induced in leaf tissues during tuberization in E1-3.