A Developed Accurate Digital PCR Detection Technology in Food Safety

2016 ◽  
pp. 123-141
Author(s):  
Wentao Xu
Keyword(s):  
Food Safety ◽  
Digital Pcr ◽  
Pcr Detection ◽  
Detection Technology

scholarly journals Study on microbial detection technology in food safety

2021 ◽  
Vol 233 ◽  
pp. 02029
Author(s):  
Xindi Zhang
Keyword(s):  
Food Safety ◽  
Rapid Development ◽  
Enzyme Linked Immunosorbent Assay ◽  
Safety Testing ◽  
Microbial Detection ◽  
Detection Technology ◽  
Atp Bioluminescence ◽  
Quality Of Food

Economic development has not only led to the steady development of the gross national economy, but also provided a fundamental guarantee for the life of the residents at this stage. However, with the rapid development of economy, people’s attention to hidden safety problems has gradually shifted from big problems to “small details” of food safety. At the same time, in order to reduce the health problems of consumers in the process of eating products, we should start from the source of food, and use microbial technology in the current food safety testing, so as to fundamentally improve the quality of food safety. At present, PCR, impedance, ATP bioluminescence, lamp and enzyme-linked immunosorbent assay are widely used. In this paper, the role of microbial detection technology was described, and the application of microbial detection technology in food safety detection was analyzed in depth, hoping to provide a reference for ensuring food safety through the promotion of microbial detection technology.

scholarly journals Microdroplet Digital PCR: Detection and Quantitation of Biomarkers in Archived Tissue and Serial Plasma Samples in Patients with Lung Cancer

2015 ◽  
Vol 10 (1) ◽  
pp. 212-217 ◽  
Author(s):  
Fangfei Gao ◽  
Ester Pfeifer ◽  
Hassan Farah ◽  
Elena Karampini ◽  
Divyanshu Dua ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Digital Pcr ◽  
Pcr Detection ◽  
Plasma Samples

scholarly journals A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples

2016 ◽  
Vol 17 (3) ◽  
pp. 402 ◽  
Author(s):  
Pengyu Zhu ◽  
Chenguang Wang ◽  
Kunlun Huang ◽  
Yunbo Luo ◽  
Wentao Xu
Keyword(s):  
Detection System ◽  
Digital Pcr ◽  
Pcr Detection ◽  
Absolute Quantitation ◽  
The Absolute

scholarly journals Analysis on Key Ideas of Research Progress in Biotechnology Crop Food Detection Technology

2020 ◽  
Vol 145 ◽  
pp. 01007
Author(s):  
Dong Tianfei
Keyword(s):  
Food Safety ◽  
Research Progress ◽  
Safety Testing ◽  
Safety Issues ◽  
Food Testing ◽  
Detection Technology ◽  
Food Detection ◽  
Testing Technology ◽  
Quality Of Food

Food not only provides nutrition to people, maintains vitality, but also it is related to people’s health. In view of the fact that current food safety issues are still common, it is very important to do a good job in food safety testing. Biotech crop food is a product that can be directly consumed. In order to improve people’s trust in such products, scientific biotechnology testing methods can improve the efficiency and quality of food testing and ensure people eat food safely. This paper will analyze the basic types of food testing technology, and then introduce the application of several key detection technologies in biotech crop food testing, and discuss the shortcomings in current food testing, and do a brief overview of future development on the biotech crop food testing technology.

scholarly journals Application of digital PCR (dPCR) in the detection of Covid-19 in food

2021 ◽  
Vol 271 ◽  
pp. 02022
Author(s):  
Hua Liu ◽  
Shanti dwita Lestari
Keyword(s):  
Pathogenic Bacteria ◽  
Low Cost ◽  
Virus Detection ◽  
Digital Pcr ◽  
Absolute Quantification ◽  
Detection Methods ◽  
Multiple Target ◽  
Detection Technology ◽  
Virus Safety ◽  
Pcr Technology

Covid-19 detection in food is an effective solution to ensure the accurate detection rate of Covid-19. The difficulties and detection methods of food virus safety detection and the feasibility of digital PCR detection technology are analyzed. The main parameters and characteristics of dPCR technology and other PCR technologies are compared. The application of dPCR technology in the detection of food viruses and pathogenic bacteria, the application of dPCR technology in the preparation and purity verification of Covid-19 RNA reference material, and the steps and methods of dPCR technology in food testing Covid-19 were expounded. Compared with traditional detection methods, digital PCR technology has great advantages in virus detection limit and stability. dPCR will develop towards high flux and automation, and achieve the absolute quantification of multiple target sequences at low cost. It will help to play a crucial role in the detection of covid-19 in food.

scholarly journals Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for Prediction of Embryo Development Potential

2021 ◽  
Vol Volume 14 ◽  
pp. 521-531
Author(s):  
Qing Zhang ◽  
Hong Ji ◽  
Jian Shi ◽  
Longmei Wang ◽  
Lu Ding ◽  
...  
Keyword(s):  
Embryo Development ◽  
Embryo Culture ◽  
Culture Medium ◽  
Digital Pcr ◽  
Pcr Detection ◽  
Development Potential ◽  
Embryo Culture Medium

scholarly journals A New Strategy for the Detection of Chicken Infectious Anemia Virus Contamination in Attenuated Live Vaccine by Droplet Digital PCR

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Qiuchen Li ◽  
Yubiao Zhang ◽  
Fanfeng Meng ◽  
Hui Jiang ◽  
Guanlong Xu ◽  
...  
Keyword(s):  
Digital Pcr ◽  
Economic Losses ◽  
Lymphoid Tissues ◽  
Detection Technology ◽  
Chicken Infectious Anemia Virus ◽  
New Strategy ◽  
Chicken Infectious Anemia ◽  
Polymerase Chain ◽  
Anemia Virus ◽  
Digital Polymerase Chain Reaction

Chicken infectious anemia virus (CIAV) causes the atrophy of bone marrow hematopoietic and lymphoid tissues in chicks, leading to huge economic losses all over the world. The using of attenuated vaccine contaminated with CIAV increased the mortality and the pathogenicity of other diseases in many farms. However, it is difficult to detect the CIAV contamination by general detection technology due to the extremely low dose of CIAV in vaccines. In this study, we established a new method called droplet digital Polymerase Chain Reaction (ddPCR) to detect CIAV contamination of vaccines more sensitively and accurately. The lowest detection limitation of this method is 2.4 copies of CIAV plasmid or CIAV contamination at 0.1 EID50/1000 feathers in vaccines without any positive signals of other viruses. Besides, the sensitivity of ddPCR is 100 times greater than that of conventional PCR and 10 times greater than that of real-time PCR. The ddPCR technique is more sensitive and more intuitive. Therefore, it could be valuable for the detection of CIAV contamination in vaccines.

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