Potential of Biopriming in Enhancing Crop Productivity and Stress Tolerance

2018 ◽  
pp. 127-145 ◽  
Author(s):  
Ahmad Mahmood ◽  
Ryota Kataoka
2021 ◽  
Author(s):  
Kota Vamsee Raja ◽  
Kalva Madhanasekhar ◽  
Vudem Dashavantha Reddy ◽  
Attipalli Ramachandra Reddy ◽  
Khareedu Venkateswara Rao

AbstractWorld-wide crop productivity is hugely impacted by diverse eco-environmental conditions. In the present investigation, activation tagged (AT) lines of rice endowed with improved agronomic attributes have been analyzed for tolerance to salinity stress besides identification of genes associated with these attributes. Under salinity stress conditions, AT lines exhibited increased seed germination rates, improved plant growth and development at vegetative and reproductive stages as compared to wild-type (WT) plants. Furthermore, AT lines disclosed enhanced plant water content, photosynthetic efficiency, stomatal conductance, water use efficiency and maximum quantum yield when compared to WT plants, leading to improved yields and delayed onset of stress symptoms. Moreover, AT lines revealed effective antioxidant systems causing decreased accumulation of reactive oxygen species and delayed salinity stress symptoms compared to WT plants. Reduced accumulation of malondialdehyde with concomitant increases in proline and soluble sugars of AT lines further endorsing their improved stress tolerance levels. TAIL and qRT-PCR analyses of AT lines revealed Ds element integrations at different loci and respective overexpression of identified candidate genes involved in various aspects of plant development and stress tolerance. Accordingly, the AT lines plausibly serve as a rare genetic resource for fortifying stress tolerance and productivity traits of elite rice cultivars.HighlightActivation tagged lines of rice endowed with improved agronomic attributes have been analyzed for tolerance to salinity stress besides identification and expression analysis of genes associated with these attributes.


2022 ◽  
Vol 46 (1) ◽  
Author(s):  
Almoataz Bellah Ali El-Mouhamady ◽  
Abdul Aziz M. Gad ◽  
Ghada S. A. Abdel Karim

Abstract Background Water stress, specifically the limited water resources needed to grow strategic crops, especially rice, poses a great threat to crop productivity. So, it was imperative that scientists all work together to try genetically improving the rice for drought tolerance in light of these environmental challenges. The aim of this study is trying to know the genetic behavior responsible for water-deficit tolerance in rice genotypes but at the molecular level. Moreover, this attempt will be an important leap in the process of genetic improvement in rice for water stress tolerance in Egypt. Results Twenty-three rice genotypes including eight parents and their fifteen F1 crosses or (the first hybrid generation) by line X tester analysis were evaluated for water stress tolerance during two experiments (the control and drought experiment) besides some molecular–biochemical studies for eight parents and the highest selected five crosses for water stress tolerance. The research revealed that five rice crosses out of fifteen hybrids were highly tolerant to water stress compared to the normal conditions. Data of biochemical markers indicated the presence of bands that are considered as molecular genetic markers for water-deficit tolerance in some rice genotypes, and this is the scientific progress achieved in this research. This was evident by increasing the density and concentration of SDS-protein electrophoresis besides enhancing the activities of peroxidase (POD) and polyphenol oxidase (PPO) under water-deficit conditions, which confirmed the tolerance of drought stress in the eight rice genotypes and the best five crosses from the first hybrid generation. Conclusion The five promising and superior rice hybrids showed an unparalleled tolerance to water stress in all evaluated traits under water stress treatment compared to the standard experiment. Also, biochemical and molecular parameters evidence confirmed the existence of unquestionable evidence that it represents the main nucleus for producing rice lines tolerated for drought stress under Egyptian conditions.


Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 146 ◽  
Author(s):  
Xiaoshuang Li ◽  
Yuqing Liang ◽  
Bei Gao ◽  
Meiheriguli Mijiti ◽  
Tohir A. Bozorov ◽  
...  

Drought and salinity are major factors limiting crop productivity worldwide. DREB (dehydration-responsive element-binding) transcription factors play important roles in plant stress response and have been identified in a wide variety of plants. Studies on DREB are focused on the A-1 (DREB1) and A-2 (DREB2) groups. Studies on A-5 group DREBs, which represent a large proportion of the DREB subfamily, is limited. In this study, we characterized and analyzed the stress tolerance function of ScDREB10, an A-5c type DREB gene from the desert moss Syntrichia caninervis. Transactivation assay in yeast showed that ScDREB10 had transactivation activity. Transient expression assay revealed that ScDREB10 was distributed both in the nucleus and cytosol of tobacco leaf epidermal cells. Overexpression of ScDREB10 significantly increased the germination percentage of Arabidopsis seeds under osmotic and salt stresses, and improved the osmotic and salt stress tolerances of Arabidopsis at the seedling stage and is associated with the expression of downstream stress-related genes and improved reactive oxygen species (ROS) scavenging ability. Our study provides insight into the molecular mechanism of stress tolerance of A-5 type DREB proteins, as well as providing a promising candidate gene for crop salt and drought stress breeding.


Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2622
Author(s):  
Lu Wei ◽  
Jerome Jeyakumar John Martin ◽  
Haiqing Zhang ◽  
Ruining Zhang ◽  
Hongxing Cao

Oil palm crops are the most important determinant of the agricultural economy within the segment of oilseed crops. Oil palm growing in their natural habitats are often challenged simultaneously by multiple stress factors, both abiotic and biotic that limit crop productivity and are major constraints to meeting global food demands. The stress-tolerant oil palm crops that mitigate the effects of abiotic stresses on crop productivity are crucially needed to sustain agricultural production. Basal stem rot threatens the development of the industry, and the key to solving the problem is to breed new oil palm varieties resistant to adversity. This has created a need for genetic improvement which involves evaluation of germplasm, pest and disease resistance, earliness and shattering resistance, quality of oil, varieties for different climatic conditions, etc. In recent years, insights into physiology, molecular biology, and genetics have significantly enhanced our understanding of oil palm response towards such stimuli as well as the reason for varietal diversity in tolerance. In this review, we explore the research progress, existing problems, and prospects of oil palm stress resistance-based physiological mechanisms of stress tolerance as well as the genes and metabolic pathways that regulate stress response.


2022 ◽  
pp. 377-398
Author(s):  
Ratnakumar Pasala ◽  
Ramesh Kulasekaran ◽  
Brij Bihari Pandey ◽  
C.H.L.N. Manikanta ◽  
K. Gopika ◽  
...  

Author(s):  
Ramesh Katam ◽  
Nitya Murthy ◽  
Sedigheh Shokri ◽  
Suresh Lokhande ◽  
Prashanth Suravajhala ◽  
...  

Water stress (WS) and heat stress (HS) have a negative effect on soybean plant growth and crop productivity. During WS, soybean plants opt for survival through ion homeostasis and the conformations of proteins are disconcerted as plant cells lose water while HS leads to difficulties in flowering and fruiting. Some of these changes include oxidative stress leading to destruction of photosynthetic apparatus, macromolecules within cells and the onset of complex signaling cascades. Changes in the physiological characteristics, proteome, and certain metabolites were investigated on molecular and cellular functions, two soybean cultivars were exposed to different heat and water stress conditions independently and in combination. Leaf protein composition was studied using 2-DE and complemented with MALDI TOF mass spectrometry. Thirty-nine proteins were significantly altered in their relative abundance in response to WS, HS and combined WS+HS in both cultivars. Functional analysis revealed a majority of heat responsive-proteins were up regulated during HS and combined stress (WS+HS) while these proteins were down regulated to WS in tolerant cultivar with heat shock proteins generally down regulated to all levels of stress in DS cultivar. Protein MED37C, a probable mediator of RNA polymerase transcription II yielded potential protein interactors partners in Arabidopsis and our studies documents the significant impact of the protein in PI cultivar. Our study hypothesizes the plant’s development of cross-stress tolerance and will help foster the ongoing ventures in genetic modifications in stress tolerance.


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