Recent Progress in Vascular Tissue-Engineered Blood Vessels

2018 ◽  
pp. 123-144 ◽  
Author(s):  
Jun Chen ◽  
Grant C. Alexander ◽  
Pratheek S. Bobba ◽  
Ho-Wook Jun
Keyword(s):  
Blood Vessels ◽  
Recent Progress ◽  
Vascular Tissue ◽  
Tissue Engineered Blood Vessels

scholarly journals Biological and engineering design considerations for vascular tissue engineered blood vessels (TEBVs)

2014 ◽  
Vol 3 ◽  
pp. 83-90 ◽  
Author(s):  
Cristina E Fernandez ◽  
Hardean E Achneck ◽  
William M Reichert ◽  
George A Truskey
Keyword(s):  
Engineering Design ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Design Considerations ◽  
Tissue Engineered Blood Vessels

Initial Assessment of Effects of Diabetes and Advanced Age on the Construction and Efficacy of Human Adipose-Derived Stem Cell-Based Tissue Engineered Blood Vessels

2013 ◽  
Author(s):  
Jeffrey T. Krawiec ◽  
Julie A. Phillippi ◽  
Brian J. Philips ◽  
Yi Hong ◽  
William R. Wagner ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Advanced Age ◽  
Initial Assessment ◽  
Great Promise ◽  
Vascular Walls ◽  
Tissue Engineered Blood Vessels ◽  
Cellular Source

Tissue engineering, the use of a biodegradable scaffold with incorporation of a cellular source, particularly with mesenchymal stem cells (MSCs) has shown great promise in developing blood vessel grafts 1. Vascular tissue engineering not only combats the important clinical need for bypass grafts but also has the potential to advance current approaches by limiting intimal hyperplasia, thrombosis, and extended cell culture times 2–5. However, despite significant progress in this field, many preclinical evaluations of tissue engineered blood vessels (TEBVs) utilize cells from donor bases that are either non-human or from humans that are healthy 1. It is therefore unclear if cells from compromised donor populations are able to function effectively as the cellular component of TEBVs. This is particularly important for MSC-based TEBVs as they rely heavily on cellular processes to remodel in vivo to a native-like structure, with the current hypothesis being that MSCs stimulate the migration of smooth muscle cells (SMCs) from the adjacent vascular walls 6,7. While some studies have noted that cellular dysfunction exists with the presence of certain conditions 8–11, it is critically important for the field of TEBVs to evaluate human cells, specifically those from patients at high risk for cardiovascular disease such as diabetics and those of advanced age.

Effect of all-trans retinoic acid and pentagalloyl glucose on smooth muscle cell elastogenesis

2021 ◽  
pp. 1-13
Author(s):  
Kaveh Sanaei ◽  
Sydney Plotner ◽  
Anson Oommen Jacob ◽  
Jaime Ramirez-Vick ◽  
Narendra Vyavahare ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Retinoic Acid ◽  
Smooth Muscle Cells ◽  
Blood Vessels ◽  
Muscle Cells ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Tissue Engineered Blood Vessels ◽  
Pentagalloyl Glucose ◽  
Vitamin A Derivative

BACKGROUND: The main objective of tissue engineering is to fabricate a tissue construct that mimics native tissue both biologically and mechanically. A recurring problem for tissue-engineered blood vessels (TEBV) is deficient elastogenesis from seeded smooth muscle cells. Elastin is an integral mechanical component in blood vessels, allowing elastic deformation and retraction in response to the shear and pulsatile forces of the cardiac system. OBJECTIVE: The goal of this research is to assess the effect of the vitamin A derivative all-trans retinoic acid (RA) and polyphenol pentagalloyl glucose (PGG) on the expression of elastin in human aortic smooth muscle cells (hASMC). METHODS: A polycaprolactone (PCL) and the gelatin polymer composite was electrospun and doped with RA and PGG. The scaffolds were subsequently seeded with hASMCs and incubated for five weeks. The resulting tissue-engineered constructs were evaluated using qPCR and Fastin assay for their elastin expression and deposition. RESULTS: All treatments showed an increased elastin expression compared to the control, with PGG treatments showing a significant increase in gene expression and elastin deposition.

Production of Extracellular Matrix Components in Tissue-Engineered Blood Vessels

2006 ◽  
Vol 12 (4) ◽  
pp. 831-842 ◽  
Author(s):  
Sepideh Heydarkhan-Hagvall ◽  
Maricris Esguerra ◽  
Gisela Helenius ◽  
Rigmor Söderberg ◽  
Bengt R. Johansson ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Blood Vessels ◽  
Extracellular Matrix Components ◽  
Tissue Engineered Blood Vessels ◽  
Matrix Components

Emulating Early Atherosclerosis in a Vascular Microphysiological System Using Branched Tissue‐Engineered Blood Vessels

2021 ◽  
pp. 2000428
Author(s):  
Jounghyun H. Lee ◽  
Zaozao Chen ◽  
Siyu He ◽  
JoyceK. Zhou ◽  
Alexander Tsai ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Tissue Engineered Blood Vessels ◽  
Early Atherosclerosis

scholarly journals Functional tissue-engineered blood vessels from bone marrow progenitor cells

2007 ◽  
Vol 75 (3) ◽  
pp. 618-628 ◽  
Author(s):  
J LIU ◽  
D SWARTZ ◽  
H PENG ◽  
S GUGINO ◽  
J RUSSELL ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Bone Marrow Progenitor Cells ◽  
Functional Tissue ◽  
Bone Marrow Progenitor ◽  
Tissue Engineered Blood Vessels

Scaffolds in tissue engineering of blood vessels

2010 ◽  
Vol 88 (9) ◽  
pp. 855-873 ◽  
Author(s):  
Divya Pankajakshan ◽  
Devendra K. Agrawal
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Biological Scaffolds ◽  
Hemodynamic Forces ◽  
Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

Bond Strength of Thermally Fused Vascular Tissue Varies With Apposition Force

2015 ◽  
Vol 137 (12) ◽  
Author(s):  
Nicholas S. Anderson ◽  
Eric A. Kramer ◽  
James D. Cezo ◽  
Virginia L. Ferguson ◽  
Mark E. Rentschler
Keyword(s):  
Bond Strength ◽  
Blood Vessels ◽  
Thermal Energy ◽  
Molecular Mechanisms ◽  
Vascular Tissue ◽  
Tissue Temperature ◽  
Tissue Fusion ◽  
Tunica Media ◽  
Adhesive Mechanics ◽  
Bond Strengths

Surgical tissue fusion devices ligate blood vessels using thermal energy and coaptation pressure, while the molecular mechanisms underlying tissue fusion remain unclear. This study characterizes the influence of apposition force during fusion on bond strength, tissue temperature, and seal morphology. Porcine splenic arteries were thermally fused at varying apposition forces (10–500 N). Maximum bond strengths were attained at 40 N of apposition force. Bonds formed between 10 and 50 N contained laminated medial layers; those formed above 50 N contained only adventitia. These findings suggest that commercial fusion devices operate at greater than optimal apposition forces, and that constituents of the tunica media may alter the adhesive mechanics of the fusion mechanism.

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