Phytopathogen Biomass as Inducer of Antifungal Compounds by Trichoderma asperellum Under Solid-State Fermentation

Abstract Paper production generates large quantities of a solid waste known as papermaking sludge (PS), which needs to be handled properly for final disposal. The high amount of this byproduct creates expensive economical costs and induces environmental and ecological risks. Therefore, it is necessary to search uses for PS, in order to reduce the negative environmental impact and to generate a more valuable byproduct. Due to the cellulolytic composition of PS, this work evaluated a solid state fermentation process using it as substrate to obtain spores of the fungus Trichoderma asperellum. Optimal conditions to obtain T. asperellum spores were: 60% water content, 3% (w/w) salts (Nutrisol P® and Nutrisol K®), inoculum concentration at 1x105 spores/g, and pasteurized or sterilized PS. Under these conditions it was possible to obtain 2.37x109 spores/g. T. asperellum spores applied directly to pepper (Capsicum anuum) seeds without PS increased significantly seedling dry mass in greenhouse assays. This work suggests an alternative, economic and abundant substrate for production of T. asperellum spores.

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Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation

Journal of Environmental Management ◽

10.1016/j.jenvman.2019.04.113 ◽

2019 ◽

Vol 243 ◽

pp. 206-217 ◽

Cited By ~ 13

Author(s):

Uchenna R. Ezeilo ◽

Chew Tin Lee ◽

Fahrul Huyop ◽

Iffah Izzati Zakaria ◽

Roswanira Abdul Wahab

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Oil Palm ◽

Cost Effective ◽

Trichoderma Asperellum ◽

Oil Palm Frond ◽

Palm Frond

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Agave atrovirens fibers as substrate and support for solid-state fermentation for cellulase production by Trichoderma asperellum

3 Biotech ◽

10.1007/s13205-016-0426-6 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 7

Author(s):

Naivy Y. Nava-Cruz ◽

Juan C. Contreras-Esquivel ◽

Miguel A. Aguilar-González ◽

Alberto Nuncio ◽

Raúl Rodríguez-Herrera ◽

...

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Cellulase Production ◽

Trichoderma Asperellum

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Cellulases production on paper and sawdust using native Trichoderma asperellum

Universitas Scientiarum ◽

10.11144/javeriana.sc23-3.cpop ◽

2018 ◽

Vol 23 (3) ◽

pp. 419-436

Author(s):

Y Maritza Zapata ◽

Angelica Galviz-Quezada ◽

Víctor Manuel Osorio Echeverri

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Second Generation ◽

Lignocellulosic Material ◽

Trichoderma Asperellum ◽

Bioethanol Production ◽

Chemical Pretreatment ◽

Cellulosic Materials ◽

Second Generation Biofuels ◽

Glycosidic Bonds

Microbial cellulases are industrially used enzymes that catalyze the cleavage of the glycosidic bonds of cellulose. This hydrolysis yields sugars that can be used in processes such as bioethanol production. These enzymes are mainly produced by fungi belonging to the genus Trichoderma via submerged or solid state fermentation with cellulosic materials as substrates. Recent publications have increasingly demonstrated that alternatives to T.reesei enzymes in the production of second generation biofuels exist. Here, cellulolytic activities of crude extracts obtained from a native isolate of T.asperellum from coffe pulp and a strain of T.reesei were evaluated. Solid state fermentations were performed using paper and sawdust as substrates. The activities were measured after 12 days of incubation. The extracts obtained from T.reesei showed higher cellulase and endoglucanase activities (6.5 and 5.8 U/g) than those obtained using T.asperellum (5.6 and 4.1 U/g) with paper as substrate. There were no significant differences between isolates when grownon sawdust. It was possible to verify that native T.asperellum was able to produce cellulases on lignocellulosic material such as moistened paper and sawdust without having undergone a chemical pretreatment.

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The Effect of Aeration for 6-Pentyl-alpha-pyrone, Conidia and Lytic Enzymes Production by Trichoderma asperellum Strains Grown in Solid-State Fermentation

Waste and Biomass Valorization ◽

10.1007/s12649-019-00809-4 ◽

2019 ◽

Vol 11 (11) ◽

pp. 5711-5720 ◽

Cited By ~ 1

Author(s):

Rayhane Hamrouni ◽

Josiane Molinet ◽

Nathalie Dupuy ◽

Nadira Taieb ◽

Quentin Carboue ◽

...

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Amylase Activity ◽

Trichoderma Asperellum ◽

Olive Pomace ◽

Lytic Enzymes ◽

Field Crops ◽

Best Value ◽

Forced Aeration ◽

Chemical Pesticides

Abstract In recent years, the production of biopesticides has gained great attention in the scientific word because it is an important alternative to replace the much debated chemical pesticides used on the field crops. Fungal lytic enzymes, conidia and secondary metabolites like 6 pentyl-alpha-pyrone (6-PP) play a very important role in the biological control of pests. On the present study, the influence of application of air through a solid-state fermentation using three Trichoderma asperellum strains to produce conidia, 6-PP and essential enzymes were evaluated. A mix of vine shoots, potatoes flour, jatropha, olive pomace and olive oil as substrates was used. T. asperellum TV104 showed the best 6-PP production (3.06 ± 0.15 mg g DM−1), cellulases activities (34.3 ± 0.4 U g−1), and amylase activity (46.3 ± 0.6 U g−1) however, T. asperellum TF1 produced the higher levels of lipase (30.6 ± 0.3 U g−1), under air conditions. The production of these same enzymes was less efficient without the application of forced aeration. The forced aeration increased the conidia production, the best value was observed with T. asperellum TF1 (2.23 ± 0.07 × 109 g DM−1). Graphic Abstract

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