scholarly journals Surfactant, Nitrogen and Carbon Media Optimization for Trichoderma Asperellum LBKURCC1 Laccase Production by Flask Solid State Fermentation of Rice Straw

2019 ◽  
Vol 1351 ◽  
pp. 012030 ◽  
Author(s):  
A G Rahayu ◽  
P S Utama ◽  
Y Nurulita ◽  
M Miranti ◽  
T T Nugroho
Keyword(s):  
Solid State ◽  
Rice Straw ◽  
Solid State Fermentation ◽  
Laccase Production ◽  
Media Optimization ◽  
Trichoderma Asperellum

Utilization of rice straw for laccase production by Streptomyces psammoticus in solid-state fermentation

2007 ◽  
Vol 34 (10) ◽  
pp. 665-674 ◽  
Author(s):  
Kizhakkedathu Narayanan Niladevi ◽  
Rajeev Kumar Sukumaran ◽  
Parukuttyamma Prema
Keyword(s):  
Solid State ◽  
Rice Straw ◽  
Solid State Fermentation ◽  
Laccase Production

scholarly journals OPTIMISASI PRODUKSI ENZIM LAKASE PADA FERMENTASI KULTUR PADAT MENGGUNAKAN JAMUR PELAPUK PUTIH Marasmius sp. : PENGARUH UKURAN PARTIKEL, KELEMBAPAN, DAN KONSENTRASI Cu

2016 ◽  
Vol 3 (02) ◽  
Author(s):  
Cornelius Damar Hanung ◽  
Ronald Osmond ◽  
Hendro Risdianto ◽  
Sri Harjati Suhardi ◽  
Tjandra Setiadi
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Rice Straw ◽  
Solid State Fermentation ◽  
Carbon Sources ◽  
White Rot Fungi ◽  
Batch Process ◽  
Solid Substrate ◽  
White Rot

White rot fungi of Marasmius sp. is a fungus which produce laccase in high activity. Laccase is one of the ligninolityc enzymes that capable to degrade lignin. This ability can be used for the pretreatment of lignocellulosic materials in the bioethanol production. Laccase was produced in flask by batch process using Solid State Fermentation (SSF). The optimisation was conducted by statistically of full factorial design. The particle size, moisture content, and Cu concentration were investigated in this study. Rice straw was used as solid substrate and the glycerol was used as the carbon sources in modified Kirk medium. The results showed that particle size of rice straw did not affect significantly to the enzyme activity. The highest laccase activity of 4.45 IU/g dry weight was obtained at the moisture content of 61% and Cu concentration of 0.1 mM.Keywords: laccase, Marasmius sp., optimisation, rice straw, solid state fermentation ABSTRAKJamur pelapuk putih, Marasmius sp. merupakan jamur yang menghasilkan enzim lakase dengan aktivitas tinggi. Lakase merupakan enzim ligninolitik yang dapat mendegradasi lignin. Kemampuan ini dapat digunakan untuk proses pengolahan awal bahan lignoselulosa pada pembuatan bioetanol. Produksi lakase dilakukan dalam labu dengan modus batch menggunakan fermentasi kultur padat. Optimisasi produksi enzim lakase dengan metode fermentasi padat dilakukan dengan  rancangan percobaan faktorial penuh. Pengaruh ukuran partikel, kelembapan, dan konsentrasi Cu diuji dengan medium penyangga jerami dengan menambahkan gliserol dalam medium Kirk termodifikasi sebagai sumber karbon. Penelitian ini menunjukkan bahwa ukuran jerami tidak berpengaruh signifikan terhadap aktivitas enzim. Aktivitas enzim lakase maksimum terjadi pada saat kelembapan 61% dan konsentrasi Cu 0,1 mM dengan aktivitas enzim lakase/berat kering tertinggi mencapai 4,45 IU/g.Kata kunci: lakase, Marasmius sp., optimisasi, jerami, fermentasi kultur padat

scholarly journals Solid-state fermentation of paper sludge to obtain spores of the fungus Trichoderma asperellum

2019 ◽  
Vol 3 (2) ◽  
pp. 71-77
Author(s):  
Rosa Dorta-Vásquez ◽  
Oscar Valbuena ◽  
Domenico Pavone-Maniscalco
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Fermentation Process ◽  
Dry Mass ◽  
Paper Sludge ◽  
Ecological Risks ◽  
Trichoderma Asperellum ◽  
Optimal Conditions ◽  
Inoculum Concentration ◽  
Negative Environmental Impact

Abstract Paper production generates large quantities of a solid waste known as papermaking sludge (PS), which needs to be handled properly for final disposal. The high amount of this byproduct creates expensive economical costs and induces environmental and ecological risks. Therefore, it is necessary to search uses for PS, in order to reduce the negative environmental impact and to generate a more valuable byproduct. Due to the cellulolytic composition of PS, this work evaluated a solid state fermentation process using it as substrate to obtain spores of the fungus Trichoderma asperellum. Optimal conditions to obtain T. asperellum spores were: 60% water content, 3% (w/w) salts (Nutrisol P® and Nutrisol K®), inoculum concentration at 1x105 spores/g, and pasteurized or sterilized PS. Under these conditions it was possible to obtain 2.37x109 spores/g. T. asperellum spores applied directly to pepper (Capsicum anuum) seeds without PS increased significantly seedling dry mass in greenhouse assays. This work suggests an alternative, economic and abundant substrate for production of T. asperellum spores.

scholarly journals Solid-state fermentation with orange waste: optimization of Laccase production from Pleurotus pulmonarius CCB-20 and decolorization of synthetic dyes

2020 ◽  
Vol 42 ◽  
pp. e52699 ◽  
Author(s):  
Alex Graça Contato ◽  
Fabíola Dorneles In´ácio ◽  
Tatiane Brugnari ◽  
Caroline Aparecida Vaz de Araújo ◽  
Giselle Maria Maciel ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Laccase Activity ◽  
Fermentation Medium ◽  
Laccase Production ◽  
Synthetic Dyes ◽  
Pleurotus Pulmonarius ◽  
Biotechnological Potential ◽  
Orange Waste ◽  
Oxidoreductase Enzymes

Laccases are oxidoreductase enzymes that have the ability to oxidize phenolic substrates. Its biotechnological potential has been greatly explored in many areas as biotechnology industry, bioremediation of dyes, food industry and environmental microbiology. The aim of this study was maximize the laccase production by Pleurotus pulmonarius (Fr.) Quélet in solid-state fermentation (SSF) using orange waste as substrate. After optimization the capability of the crude laccase to decolorize dyes was analyzed. The fermentation medium in the solid-state was optimized by applying a factorial design. After statistics optimization, laccase activity increased two times. The laccase activity appears to be correlated with the ability of crude extract to decolorize some industrial dyes. The optimized laccase was characterized with respect to optimum pH, influence of temperature and salts. Our results demonstrate that P. pulmonarius was an efficient producer of an important industrial enzyme, laccase, in a cheap solid-state system using orange waste as substrate.

scholarly journals Agave atrovirens fibers as substrate and support for solid-state fermentation for cellulase production by Trichoderma asperellum

3 Biotech ◽  
2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Naivy Y. Nava-Cruz ◽  
Juan C. Contreras-Esquivel ◽  
Miguel A. Aguilar-González ◽  
Alberto Nuncio ◽  
Raúl Rodríguez-Herrera ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Cellulase Production ◽  
Trichoderma Asperellum

scholarly journals Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse byPleurotus ostreatusin Solid-State Fermentation

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Susan Grace Karp ◽  
Vincenza Faraco ◽  
Antonella Amore ◽  
Luiz Alberto Junior Letti ◽  
Vanete Thomaz Soccol ◽  
...  
Keyword(s):  
Solid State ◽  
Ferulic Acid ◽  
Ammonium Sulfate ◽  
Sugarcane Bagasse ◽  
Copper Sulfate ◽  
Yeast Extract ◽  
Solid State Fermentation ◽  
Laccase Activity ◽  
Oxidative Enzymes ◽  
Laccase Production

Laccases are oxidative enzymes related to the degradation of phenolic compounds, including lignin units, with concomitant reduction of oxygen to water. Delignification is a necessary pretreatment step in the process of converting plant biomass into fermentable sugars. The objective of this work was to optimize the production of laccases and to evaluate the delignification of sugarcane bagasse byPleurotus ostreatusin solid-state fermentation. Among eight variables (pH, water activity, temperature, and concentrations of CuSO4, (NH4)2SO4, KH2PO4, asparagine, and yeast extract), copper sulfate and ammonium sulfate concentrations were demonstrated to significantly influence laccase production. The replacement of ammonium sulfate by yeast extract and the addition of ferulic acid as inducer provided increases of 5.7- and 2.0-fold, respectively, in laccase activity. Optimization of laccase production as a function of yeast extract, copper sulfate, and ferulic acid concentrations was performed by response surface methodology and optimal concentrations were 6.4 g/L, 172.6 μM, and 1.86 mM, respectively. Experimentally, the maximum laccase activity of 151.6 U/g was produced at the 5th day of solid-state fermentation. Lignin content in sugarcane bagasse was reduced from 31.89% to 26.36% after 5 days and to 20.79% after 15 days by the biological treatment of solid-state fermentation.

Sign in / Sign up

Export Citation Format

Share Document