scholarly journals PRODUCTION OF Trichoderma asperellum T8a SPORES BY A “HOME-MADE” SOLID-STATE FERMENTATION OF MANGO INDUSTRIAL WASTES

BioResources ◽  
2012 ◽  
Vol 7 (4) ◽  
Author(s):  
Sergio De los Santos-Villalobos ◽  
Luis Ernesto Hernández-Rodríguez ◽  
Francisco Villaseñor-Ortega ◽  
Juan José Peña-Cabriales
2019 ◽  
Vol 3 (2) ◽  
pp. 71-77
Author(s):  
Rosa Dorta-Vásquez ◽  
Oscar Valbuena ◽  
Domenico Pavone-Maniscalco

Abstract Paper production generates large quantities of a solid waste known as papermaking sludge (PS), which needs to be handled properly for final disposal. The high amount of this byproduct creates expensive economical costs and induces environmental and ecological risks. Therefore, it is necessary to search uses for PS, in order to reduce the negative environmental impact and to generate a more valuable byproduct. Due to the cellulolytic composition of PS, this work evaluated a solid state fermentation process using it as substrate to obtain spores of the fungus Trichoderma asperellum. Optimal conditions to obtain T. asperellum spores were: 60% water content, 3% (w/w) salts (Nutrisol P® and Nutrisol K®), inoculum concentration at 1x105 spores/g, and pasteurized or sterilized PS. Under these conditions it was possible to obtain 2.37x109 spores/g. T. asperellum spores applied directly to pepper (Capsicum anuum) seeds without PS increased significantly seedling dry mass in greenhouse assays. This work suggests an alternative, economic and abundant substrate for production of T. asperellum spores.


2018 ◽  
Vol 37 (2) ◽  
pp. 149-156 ◽  
Author(s):  
C. Marzo ◽  
A.B. Díaz ◽  
I. Caro ◽  
A. Blandino

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.


3 Biotech ◽  
2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Naivy Y. Nava-Cruz ◽  
Juan C. Contreras-Esquivel ◽  
Miguel A. Aguilar-González ◽  
Alberto Nuncio ◽  
Raúl Rodríguez-Herrera ◽  
...  

Author(s):  
Reynaldo De la Cruz-Quiroz ◽  
Juan Alberto Ascacio-Valdés ◽  
Raúl Rodríguez-Herrera ◽  
Sevastianos Roussos ◽  
Cristóbal N. Aguilar

2018 ◽  
Vol 23 (3) ◽  
pp. 419-436
Author(s):  
Y Maritza Zapata ◽  
Angelica Galviz-Quezada ◽  
Víctor Manuel Osorio Echeverri

Microbial cellulases are industrially used enzymes that catalyze the cleavage of the glycosidic bonds of cellulose. This hydrolysis yields sugars that can be used in processes such as bioethanol production. These enzymes are mainly produced by fungi belonging to the genus Trichoderma via submerged or solid state fermentation with cellulosic materials as substrates. Recent publications have increasingly demonstrated that alternatives to T.reesei enzymes in the production of second generation biofuels exist. Here, cellulolytic activities of crude extracts obtained from a native isolate of T.asperellum from coffe pulp and a strain of T.reesei were evaluated. Solid state fermentations were performed using paper and sawdust as substrates. The activities were measured after 12 days of incubation. The extracts obtained from T.reesei showed higher cellulase and endoglucanase activities (6.5 and 5.8 U/g) than those obtained using T.asperellum (5.6 and 4.1 U/g) with paper as substrate. There were no significant differences between isolates when grownon sawdust. It was possible to verify that native T.asperellum was able to produce cellulases on lignocellulosic material such as moistened paper and sawdust without having undergone a chemical pretreatment.


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