Quality Standards of Stem Cell Sources for Clinical Treatment of Neurodegenerative Diseases

2020 ◽  
pp. 9-19
Author(s):  
Fabin Han ◽  
Yongquan Gu ◽  
Hui Zhao ◽  
Lin Chen
Keyword(s):  
Stem Cell ◽  
Neurodegenerative Diseases ◽  
Quality Standards ◽  
Clinical Treatment ◽  
Cell Sources

scholarly journals Rapid Formation of Stem Cell Spheroids Using Two-Dimensional MXene Particles

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 957
Author(s):  
JunHwee Jang ◽  
Eun-Jung Lee
Keyword(s):  
Stem Cell ◽  
Mass Production ◽  
Processing Time ◽  
Activity Levels ◽  
Two Dimensional ◽  
Spheroid Formation ◽  
Cell Spheroids ◽  
Tissue Healing ◽  
Rapid Formation ◽  
Cell Sources

Cell spheroids have been studied as a biomimic medicine for tissue healing using cell sources. Rapid cell spheroid production increases cell survival and activity as well as the efficiency of mass production by reducing processing time. In this study, two-dimensional MXene (Ti3C2) particles were used to form mesenchymal stem cell spheroids, and the optimal MXene concentration, spheroid-production times, and bioactivity levels of spheroid cells during this process were assessed. A MXene concentration range of 1 to 10 μg/mL induced spheroid formation within 6 h. The MXene-induced spheroids exhibited osteogenic-differentiation behavior, with the highest activity levels at a concentration of 5 μg/mL. We report a novel and effective method for the rapid formation of stem cell spheroids using MXene.

scholarly journals Emerging Stem Cell Controls: Nanomaterials and Plasma Effects

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
F. F. Borghi ◽  
A. E. Rider ◽  
S. Kumar ◽  
Z. J. Han ◽  
D. Haylock ◽  
...  
Keyword(s):  
Stem Cell ◽  
Tissue Regeneration ◽  
Materials Science ◽  
Specific Interaction ◽  
Cell Control ◽  
Nanostructured Surfaces ◽  
Plasma Effects ◽  
Cell Sources ◽  
Technological Challenge

Stem cells (SC) are among the most promising cell sources for tissue engineering due to their ability to self-renew and differentiate, properties that underpin their clinical application in tissue regeneration. As such, control of SC fate is one of the most crucial issues that needs to be fully understood to realise their tremendous potential in regenerative biology. The use of functionalized nanostructured materials (NM) to control the microscale regulation of SC has offered a number of new features and opportunities for regulating SC. However, fabricating and modifying such NM to induce specific SC response still represent a significant scientific and technological challenge. Due to their versatility, plasmas are particularly attractive for the manufacturing and modification of tailored nanostructured surfaces for stem cell control. In this review, we briefly describe the biological role of SC and the mechanisms by which they are controlled and then highlight the benefits of using a range of nanomaterials to control the fate of SC. We then discuss how plasma nanoscience research can help produce/functionalise these NMs for more effective and specific interaction with SCs. The review concludes with a perspective on the advantages and challenges of research at the intersection between plasma physics, materials science, nanoscience, and SC biology.

Stem Cell Sources

2004 ◽  
pp. 337-356 ◽  
Author(s):  
Corey Cutler ◽  
Joseph H. Antin
Keyword(s):  
Stem Cell ◽  
Cell Sources

scholarly journals Human iPSC-derived brain endothelial microvessels in a multi-well format enable permeability screens of anti-inflammatory drugs

2021 ◽  
Author(s):  
Sven Fengler ◽  
Birgit Kurkowsky ◽  
Sanjeev Kumar Kaushalya ◽  
Wera Roth ◽  
Philip Denner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neurodegenerative Diseases ◽  
Induced Pluripotent Stem Cell ◽  
Barrier Properties ◽  
Brain Diseases ◽  
Rapid Screening ◽  
Sodium Fluorescein ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Optimizing drug candidates for blood-brain barrier (BBB) penetration in humans remains one of the key challenges and many devastating brain diseases including neurodegenerative diseases still do not have adequate treatments. So far, it has been difficult to establish state-of-the-art human stem cell derived in vitro models that mimic physiological barrier properties including a 3D microvasculature in a format that is scalable enough to screen drugs for BBB penetration in early drug development phases. To address this challenge, we established human induced pluripotent stem cell (iPSC)-derived brain endothelial microvessels in a standardized and scalable multi-well plate format. iPSC-derived brain microvascular endothelial cells (BMECs) were supplemented with primary cell conditioned media and grew to intact microvessels in 10 days of culturing. Produced microvessels show a typical BBB phenotype including endothelial protein expression, tight-junctions and polarized localization of efflux transporter. Microvessels exhibited physiological relevant trans-endothelial electrical resistance (TEER), were leak tight for 10 kDa dextran-Alexa 647 and strongly limited the permeability of sodium fluorescein (NaF). Permeability tests with reference compounds confirmed the suitability of our model as platform to identify potential BBB penetrating anti-inflammatory drugs. In summary, the here presented brain microvessel platform recapitulates physiological properties and allows rapid screening of BBB permeable anti-inflammatory compounds that has been suggested as promising substances to cure so far untreatable neurodegenerative diseases.

Stem Cell Sources

1999 ◽  
pp. 27-34 ◽  
Author(s):  
G. Van Zant
Keyword(s):  
Stem Cell ◽  
Cell Sources
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