Quality Control Measures in Short Tandem Repeat (STR) Analysis

2021 ◽  
pp. 1-18
Author(s):  
Heather Miller Coyle
Keyword(s):  
Quality Control ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Control Measures ◽  
Str Analysis ◽  
Short Tandem

Evaluation genotypes of cancer cell lines HCC1954 and SiHa by short tandem repeat (STR) analysis and DNA sequencing

2018 ◽  
Vol 45 (6) ◽  
pp. 2689-2695 ◽  
Author(s):  
Jiewen Fu ◽  
Jingliang Cheng ◽  
Xiaoyan Liu ◽  
Jun Li ◽  
Chunli Wei ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Cell Lines ◽  
Cancer Cell ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Cancer Cell Lines ◽  
Str Analysis ◽  
Short Tandem

Variant alleles observed in short tandem repeat (STR) analysis of random Indian population

2019 ◽  
Vol 19 (1) ◽  
pp. 94
Author(s):  
Monika Chakravarty ◽  
Nidhi Singh ◽  
Damini Varshney ◽  
Dhruw Sharma ◽  
Naresh Kumar ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Indian Population ◽  
Str Analysis ◽  
Variant Alleles ◽  
Short Tandem

Automated short tandem repeat (STR) analysis in forensic casework — a strategy for the future

1995 ◽  
Vol 16 (1) ◽  
pp. 1543-1552 ◽  
Author(s):  
Peter Gill ◽  
Colin P. Kimpton ◽  
Andrew Urquhart ◽  
Nicola Oldroyd ◽  
Emma S. Millican ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Str Analysis ◽  
Forensic Casework ◽  
The Future ◽  
Short Tandem

scholarly journals Utilization of Short Tandem Repeat Analysis to Resolve Specimen Mislabeling in the Anatomic Pathology Laboratory

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S112-S112
Author(s):  
T Tenney ◽  
B Robinson ◽  
H C Sullivan
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Single Patient ◽  
Pathology Laboratory ◽  
Str Analysis ◽  
Hematopoietic Stem ◽  
Anatomic Pathology ◽  
The Given ◽  
Short Tandem

Abstract Introduction/Objective A mislabeled specimen is an example of preanalytical error that can have significant consequences on patient care. These errors can be difficult to detect and resolve. One method to confirm genetic identity is short tandem repeat (STR) analysis, which is utilized in forensic investigations, paternity studies, and post- hematopoietic stem cell transplantation monitoring. Herein we present application of STR analysis to resolve a suspected specimen mislabeling prior to receipt in our anatomic pathology laboratory. Methods/Case Report DNA was extracted from paraffin embedded tissues. Chimerism testing was performed by STR analysis using the Globalfiler (ThermoFisher Scientific) and analyzed by Chimermarker (Softgenetics) automated chimerism software. Results (if a Case Study enter NA) Colon biopsies were received for a single patient (#1) with two requisition forms. Each specimen (A-F) was labeled with the patient’s name, with specimens A-D noted on first page of requisition and specimens E-F on the second requisition page. After the case was signed out, the lab was contacted looking for biopsy results on another patient (#2) who was seen on the same day as patient #1. Review of all the patients seen in the endoscopy suite on the given date raised suspicion that specimens E-F from the second page of the requisition actually pertained to patient #2. STR analysis performed on specimens confirmed that specimens E-F were genetically distinct from those labelled A-D. Tissue from a subsequent biopsy on patient #2 was analyzed by STR testing, which was identical to STR results performed on specimens E-F. Conclusion Here we utilized STR testing to resolve a suspected mislabeled specimen, allowing the appropriate diagnosis to be attributed to the correct patient. This is a unique application of a common method, which could be implemented in anatomic pathology laboratories to resolve cases of specimen mix-ups.

scholarly journals Individual Identification with Short Tandem Repeat Analysis and Collection of Secondary Information Using Microbiome Analysis

Genes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 85
Author(s):  
Solip Lee ◽  
Heesang You ◽  
Songhee Lee ◽  
Yeongju Lee ◽  
Hee-Gyoo Kang ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Individual Identification ◽  
Future Research ◽  
Str Analysis ◽  
Legal Evidence ◽  
Advantages And Disadvantages ◽  
Secondary Information ◽  
Generation Sequencing ◽  
Short Tandem

Forensic investigation is important to analyze evidence and facilitate the search for key individuals, such as suspects and victims in a criminal case. The forensic use of genomic DNA has increased with the development of DNA sequencing technology, thereby enabling additional analysis during criminal investigations when additional legal evidence is required. In this study, we used next-generation sequencing to facilitate the generation of complementary data in order to analyze human evidence obtained through short tandem repeat (STR) analysis. We examined the applicability and potential of analyzing microbial genome communities. Microbiological supplementation information was confirmed for two of four failed STR samples. Additionally, the accuracy of the gargle sample was confirmed to be as high as 100% and was highly likely to be classified as a body fluid sample. Our experimental method confirmed that anthropological and microbiological evidence can be obtained by performing two experiments with one extraction. We discuss the advantages and disadvantages of using these techniques, explore prospects in the forensic field, and highlight suggestions for future research.

A valveless microfluidic device for integrated solid phase extraction and polymerase chain reaction for short tandem repeat (STR) analysis

The Analyst ◽  
2011 ◽  
Vol 136 (9) ◽  
pp. 1928 ◽  
Author(s):  
Kristin A. Hagan ◽  
Carmen R. Reedy ◽  
Joan M. Bienvenue ◽  
Alison H. Dewald ◽  
James P. Landers
Keyword(s):  
Polymerase Chain Reaction ◽  
Solid Phase Extraction ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Solid Phase ◽  
Phase Extraction ◽  
Chain Reaction ◽  
Str Analysis ◽  
Polymerase Chain ◽  
Short Tandem

The utility of an erythroblast scoring system and gender-independent short tandem repeat (STR) analysis for the detection of aneuploid fetal cells in maternal blood

2005 ◽  
Vol 25 (7) ◽  
pp. 586-591 ◽  
Author(s):  
Dong Hyun Cha ◽  
Kiarash Khosrotehrani ◽  
Diana W. Bianchi ◽  
Kirby L. Johnson
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Scoring System ◽  
Maternal Blood ◽  
Str Analysis ◽  
Fetal Cells ◽  
And Gender ◽  
Short Tandem

scholarly journals Disputed paternity presumption in Burkina Faso: determination of the biological fathers of children using ABO-rhesus/hemoglobin electrophoresis and STR assays

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Missa Millogo ◽  
Serge Theophile Soubeiga ◽  
Bapio Valerie Jean Telesphore Bazie ◽  
Theodora Mahoukede Zohoncon ◽  
Abdoul Karim Ouattara ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Str Analysis ◽  
Resource Limited ◽  
Biological Fathers ◽  
Short Tandem

Abstract Background In resource-limited countries, ABO, HLA, MNS, Kells, and hemoglobin electrophoresis are classic tests for the resolution of paternity disputes due to their affordable cost. The limitations of these tests in cases of disputed paternity require the use of Short Tandem Repeats (STR) for their certification. This study aimed to determine the biological fathers of children using ABO-rhesus/hemoglobin electrophoresis and STR assays in Burkina Faso, West Africa. Results Of the fourteen trios studied, the ABO-rhesus/hemoglobin electrophoresis analysis revealed ten probable inclusion cases, three exclusion cases, and one undetermined paternity. DNA STR analysis found five inclusions of paternity out of the ten probable inclusions with ABO-rhesus/hemoglobin electrophoresis assay versus nine exclusions of paternity. Conclusion This study showed that the implementation of the analysis of short tandem repeat is required to resolve increasing disputed filiation cases in Burkina Faso.

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