scholarly journals Erratum to Agrobacterium-mediated transformation and plant regeneration of buckwheat (Fagopyrum esculentum Moench.)

1992 ◽  
Vol 31 (1) ◽  
pp. 87-87
Author(s):  
Jovanka Miljuš-Djukić ◽  
Mirjana Nešković ◽  
Slavica Ninković ◽  
Radomir Crkvenjakov
Keyword(s):  
Plant Regeneration ◽  
Fagopyrum Esculentum ◽  
Agrobacterium Mediated Transformation ◽  
Fagopyrum Esculentum Moench

Agrobacterium-mediated transformation and plant regeneration of buckwheat (Fagopyrum esculentum Moench.)

1992 ◽  
Vol 29 (2) ◽  
pp. 101-108 ◽  
Author(s):  
Jovanka Miljuš-Djukić ◽  
Mirjana Nešković ◽  
Slavica Ninković ◽  
Radomir Crkvenjakov
Keyword(s):  
Plant Regeneration ◽  
Fagopyrum Esculentum ◽  
Agrobacterium Mediated Transformation ◽  
Fagopyrum Esculentum Moench

scholarly journals Methodological approaches to agrobacterium-mediated transformation of buckwheat (Fagopyrum esculentum Moench)

2020 ◽  
Author(s):  
E. L. Ilina ◽  
A. S. Kiryushkin ◽  
E. D. Guseva ◽  
K. N. Demchenko
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Cellular Response ◽  
Fagopyrum Esculentum ◽  
Agrobacterium Mediated Transformation ◽  
Composite Plants ◽  
Reporter Proteins ◽  
Methodological Approaches ◽  
Fagopyrum Esculentum Moench

The method of Agrobacterium rhizogenes-mediated transformation of buckwheat has been established; composite plants have been obtained. The distribution of the cellular response to auxin by reporter proteins with different maturation times coincides.

scholarly journals Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 768
Author(s):  
Yue Fei ◽  
Lan-Xiang Wang ◽  
Zheng-Wu Fang ◽  
Zhi-Xiong Liu
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Fagopyrum Esculentum ◽  
Cereal Crop ◽  
Embryogenic Calli ◽  
Hypocotyl Explants ◽  
Indirect Somatic Embryogenesis ◽  
Fagopyrum Esculentum Moench

Buckwheat (Fagopyrum esculentum, Family Polygonaceae) is an annual pseudo-cereal crop with healing benefits. However, the genetic improvement of common buckwheat has achieved only limited success, mainly due to buckwheat’s dimorphic flowers and heteromorphic self-incompatibility. Here, we develop a useful protocol for indirect somatic embryogenesis and subsequent plant regeneration from hypocotyl explants of F. esculentum. Firstly, the initial calli of hypocotyl explants were induced on Murashige and Skoog (MS) basal medium containing 2.0 mgL−1 2,4-D and 1.5 mgL−1 6-BA for 30 days culture, and then the yellowish white friable embryogenic calli were developed when the initial calli were transferred to fresh MS basal medium supplemented with 1.0 mgL−1 6-BA and 0.5 mgL−1 thidiazuron (TDZ)two to three times subculture at 40–60 days intervals. Subsequently, the somatic embryos were able to germinate from embryogenic callus sub-cultured on MS basal medium containing 1.0 mgL−1 6-BA and 0.5 mgL−1 TDZ with 15% potato puree for 20 days subculture. Finally, maximum mean percentage (75.75%) of somatic embryo-derived plants were obtained when the mature somatic embryos were transferred to MS basal medium without growth regulators for 40 days culture. Our result provides a useful protocol for plant regeneration and SE from hypocotyl explants of F. esculentum.

Anther culture and androgenetic plant regeneration in buckwheat (Fagopyrum esculentum Moench)

1993 ◽  
Vol 35 (3) ◽  
pp. 259-266 ◽  
Author(s):  
Borut Bohanec ◽  
Mirjana Nešković ◽  
Radmila Vujičić
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Fagopyrum Esculentum ◽  
Fagopyrum Esculentum Moench

Plant regeneration from cotyledon tissues of common buckwheat (Fagopyrum esculentum Moench)

2000 ◽  
Vol 36 (5) ◽  
pp. 358-361 ◽  
Author(s):  
Sun Hee Woo ◽  
Arun Nair ◽  
Taiji Adachi ◽  
Clayton G. Campbell
Keyword(s):  
Plant Regeneration ◽  
Fagopyrum Esculentum ◽  
Common Buckwheat ◽  
Fagopyrum Esculentum Moench

Exploring the Pharmacognostic Features, Anti-oxidant and Lipid Lowering Potential of Fagopyrum esculentum Moench. Seed

2020 ◽  
Vol 6 (2) ◽  
pp. 155-169
Author(s):  
Neeraj Panihar ◽  
Neeru Vasudeva ◽  
Sunil Sharma ◽  
Babu Lal Jangir
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Water Soluble ◽  
Fagopyrum Esculentum ◽  
Lipid Lowering ◽  
Standard Drug ◽  
Fagopyrum Esculentum Moench ◽  
Β Carotene

Background: Fagopyrum esculentum Moench. is a herb consumed as food and has medicinal value. It is a rich source of bioactive nutrients which cure and prevent many ailments. Traditionally, it is used to treat hypertension, diabetes, constipation, cancer etc. Methods and Objective: Present work illustrates morphological, microscopic and physicochemical parameters of Fagopyrum esculentum seeds as per WHO guidelines, in vitro antioxidant activity; assessed by DPPH scavenging method, hydrogen peroxide scavenging assay and β-carotene linoleic acid bleaching method and study of lipid lowering potential of the ethyl acetate and ethanol extract of seeds on normal diet fed Wistar rats. Results: Morphological studies delineated the triangular shape, dark brown colour, 8 mm length and 6 mm width of the seed. The microscopic examination of the transverse section of seed depicted features like testa or pericarp (seed coat), the endosperm, embryo and sclerenchyma cells. Study of physiochemical parameters exhibited 0.3±0.02% of foreign matter and 1.44±0.51% crude fibre content. Total ash, acid insoluble ash and water soluble ash value were 6.7±1.7%, 1.9±0.23% and 3.9± 0.31% respectively. Alcohol soluble and water soluble extractive value came out to be 65.02± 3.21 mg/g and 12.7±1.24 mg/g respectively. Foaming index was less than 100, swelling index was found to be 0.5±0.01 ml/g. Loss on drying was 4.02±1.27%. Phytochemical screening of ethyl acetate and ethanol extract revealed the presence of alkaloids, carbohydrates, phenolic compounds, phytosterols and flavonoids. Trace amount of heavy metals (arsenic, cadmium, lead, mercury) were determined by atomic absorption spectrophotometer. Pesticide residue analysis confirmed the presence of nontoxic pesticides like dimethipin, hymexazol, phenothrin-2, methoprene, triadimenol, prohydrojasmon- 1, jasmolin ii, triademinol, jasmolin i, prohydrojasmone i, cyromazine in both the extracts by gc-ms spectrometer. The ethyl acetate and ethanol extract has shown significant in-vitro antioxidant activities demonstrated by the DPPH method (IC50 = 94.37±2.51 and 216.04±4.39 μg/ml respectively), hydrogen peroxide scavenging assay (IC50 = 83.72±3.72 and 193.47±5.05 µg/ml respectively) and β-carotene bleaching method (IC50 = 100.67±4.01 and 205.39±2.89 µg/ml respectively). Lipid lowering study performed on Wistar rats demonstrated a significant (p<0.001) decrease in serum Total Cholesterol (TC), Triglyceride (TG) and increase in High Density Lipoprotein (HDL) level as compared to normal group. Both the extracts have shown a non significant difference in the level of TG as compared to standard drug atorvastatin, depicting that the efficacy of extracts is at par with that of standard drug atorvastatin. Conclusion: Pharmacognostical study of the plant can be a very good tool for identification as well as authentication of a herb. Moreover, these parameters may be helpful in the development of monograph of the plant. Pharmacological activity confirmed Fagopyrum esculentum Moench. seed to be a good antioxidant and have lipid lowering potential.

scholarly journals Effect of Natural Light Periods on Rutin, Free Amino Acid and Vitamin C Contents in the Sprouts of Common (Fagopyrum esculentum Moench) and Tartary (F. tataricum Gaertn.) Buckwheats

2006 ◽  
Vol 12 (3) ◽  
pp. 199-205 ◽  
Author(s):  
Sun-Ju KIM ◽  
Chiami KAWAHARADA ◽  
Tatsuro SUZUKI ◽  
Katsuichi SAITO ◽  
Naoto HASHIMOTO ◽  
...  
Keyword(s):  
Amino Acid ◽  
Vitamin C ◽  
Free Amino Acid ◽  
Free Amino ◽  
Fagopyrum Esculentum ◽  
Natural Light ◽  
Fagopyrum Esculentum Moench
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