The effects of varying key steps in the non-radioactive in situ hybridization protocol: a quantitative study

1995 ◽  
Vol 27 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Y. Guiot ◽  
J. Rahier
Keyword(s):  
In Situ Hybridization ◽  
Quantitative Study ◽  
Hybridization Protocol ◽  
Key Steps

scholarly journals Catalyzed Reported Deposition-Fluorescence In Situ Hybridization Protocol To Evaluate Phagotrophy in Mixotrophic Protists

2005 ◽  
Vol 71 (11) ◽  
pp. 7321-7326 ◽  
Author(s):  
Juan M. Medina-Sánchez ◽  
Marisol Felip ◽  
Emilio O. Casamayor
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Signal Amplification ◽  
Cell Attachment ◽  
Cell Loss ◽  
Alexa Fluor ◽  
Hybridization Protocol ◽  
Card Fish ◽  
First Time

ABSTRACT We describe a catalyzed reported deposition-fluorescence in situ hybridization (CARD-FISH) protocol particularly suited to assess the phagotrophy of mixotrophic protists on prokaryotes, since it maintains cell and plastid integrity, avoids cell loss and egestion of prey, and allows visualization of labeled prey against plastid autofluorescence. This protocol, which includes steps such as Lugol's-formaldehyde-thiosulfate fixation, agarose cell attachment, cell wall permeabilization with lysozyme plus achromopeptidase, and signal amplification with Alexa-Fluor 488, allowed us to detect almost 100% of planktonic prokaryotes (Bacteria and Archaea) and, for the first time, to show archaeal cells ingested by mixotrophic protists.

Higher axial-resolution and sensitivity pachytene fluorescence in situ hybridization protocol in tetraploid cotton

2009 ◽  
Vol 17 (8) ◽  
pp. 1041-1050 ◽  
Author(s):  
Kai Wang ◽  
Zaijie Yang ◽  
Changshen Shu ◽  
Jing Hu ◽  
Qiuyun Lin ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tetraploid Cotton ◽  
Axial Resolution ◽  
Hybridization Protocol

Double-Network Interpenetrating Bone Cement via in situ Hybridization Protocol

2010 ◽  
Vol 20 (22) ◽  
pp. 3997-4011 ◽  
Author(s):  
Jing Wang ◽  
Changsheng Liu ◽  
Yufei Liu ◽  
Shuo Zhang
Keyword(s):  
In Situ Hybridization ◽  
Bone Cement ◽  
Double Network ◽  
Hybridization Protocol

A versatile fluorometric in situ hybridization method for the quantitation of hairpin conformations in DNA self-assembled monolayers

The Analyst ◽  
2020 ◽  
Vol 145 (13) ◽  
pp. 4522-4531
Author(s):  
Jiale He ◽  
Xiaochen Hu ◽  
Xiaoyi Gao ◽  
Chenchen Meng ◽  
Yunchao Li ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Detection Performance ◽  
Self Assembled Monolayers ◽  
Optimal Detection ◽  
Hybridization Method ◽  
Assembled Monolayers ◽  
The Creation ◽  
Self Assembled ◽  
Hybridization Protocol

We report a versatile fluorometric in-situ hybridization protocol for quantifying hairpin conformations in DNA self-assembled monolayers on substrates, which facilitates the creation of hpDNA-based biosensors with optimal detection performance.

scholarly journals An RNA in situ hybridization protocol optimized for monocot tissue

2021 ◽  
Vol 2 (2) ◽  
pp. 100398
Author(s):  
Nora R. Zöllner ◽  
Margaret Bezrutczyk ◽  
Reinout Laureyns ◽  
Hilde Nelissen ◽  
Rüdiger Simon ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Rna In Situ Hybridization ◽  
Hybridization Protocol

scholarly journals In situ hybridization using PEG-embedded tissue and riboprobes: increased cellular detail coupled with high sensitivity.

1989 ◽  
Vol 37 (3) ◽  
pp. 389-393 ◽  
Author(s):  
D F Clayton ◽  
A Alvarez-Buylla
Keyword(s):  
In Situ Hybridization ◽  
High Sensitivity ◽  
Cresyl Violet ◽  
High Signal ◽  
Tissue Sections ◽  
Glass Slides ◽  
Cresyl Violet Staining ◽  
Rna Probes ◽  
Hybridization Protocol

We describe a procedure for preparing tissue sections by embedding in polyethylene glycol for subsequent in situ hybridization analysis using single-stranded RNA probes. Improved tissue morphology is obtained as compared to frozen sections, and the embedding procedure is milder and faster than paraffin embedding. Sections as thin as 2 microns are readily cut from PEG-embedded brain tissue. A simplified hybridization protocol (Clayton et al.: Neuron 1:249, 1988) supports the detection of even low-abundance brain mRNAs (less than or equal to 10(-4) fractional mRNA mass). By employing high stringency washes in place of ribonuclease treatment after hybridization, cell RNA is retained for cresyl violet staining, and high signal:noise ratios are achieved. Solutions to problems with section mounting and adherence to glass slides are presented. The combination of improved morphology, high signal levels, and relative simplicity should make this procedure useful in a variety of applications.

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