Rapid purification of uricase from Bacillus fastidiosus

1996 ◽  
Vol 10 (9) ◽  
pp. 709-712
Author(s):  
M. S. H. Muruke ◽  
H. J. M. Op den Camp ◽  
C. van der Drift
Keyword(s):  
Rapid Purification ◽  
Bacillus Fastidiosus

Uultrastructure of Microtubules

1972 ◽  
Vol 30 ◽  
pp. 252-253
Author(s):  
Ariaki Nagayama
Keyword(s):  
Fine Structure ◽  
Cultured Cells ◽  
Present Report ◽  
Confluent Monolayer ◽  
Purification Method ◽  
Vinca Rosea ◽  
L Cells ◽  
Antimitotic Activity ◽  
Monolayer Cultures ◽  
Rapid Purification

Vinblastine(Vb) or vincristine, alkaloid derived from Vinca rosea is known for its antimitotic activity by regrouping of microtubules into paracrystalline form within the cells. A rapid purification method of vinblastine-induced microtubular paracrystals(PC) has provided us with a fresh and pure microtubular material demonstrating the presence of a labile ATPase associated with the PC. The present report is concerned with the fine structure of purified microtubules of mammalian cultured cells.Confluent monolayer cultures of L cells were incubated for 20hrs with 10-5 M Vb (donated from Shionogi Seiyaku & Co., Osaka, Japan).

A Novel Method for the Rapid Purification of Human and Rat Fibrin(OGEN) Degradation Products in High Yields

1979 ◽  
Author(s):  
W. Nieuwenhuizen ◽  
I. A. M. van Ruijven-Vermeer ◽  
F. Haverkate ◽  
G. Timan
Keyword(s):  
Degradation Products ◽  
Complete Separation ◽  
Purification Method ◽  
Amino Terminal ◽  
Novel Method ◽  
Rapid Purification ◽  
The One ◽  
High Yields ◽  
Size Heterogeneity ◽  
D Dimer

A novel method will be described for the preparation and purification of fibrin(ogen) degradation products in high yields. The high yields are due to two factors. on the one hand an improved preparation method in which the size heterogeneity of the degradation products D is strongly reduced by plasmin digestion at well-controlled calcium concentrations. At calcium concentrations of 2mM exclusively D fragments, M.W.= 93-000 (Dcate) were formed; in the presence of 1OmM EGTA only fragments M.W.= 80.000 (D EGTA) were formed as described. on the other hand a new purification method, which includes Sephadex G-200 filtration to purify the D:E complexes and separation of the D and E fragments by a 16 hrs. preparative isoelectric focussing. The latter step gives a complete separation of D (fragments) (pH = 6.5) and E fragments (at pH = 4.5) without any overlap, thus allowing a nearly 100% recovery in this step. The overall recoveries are around 75% of the theoretical values. These recoveries are superior to those of existing procedures. Moreover the conditions of this purification procedure are very mild and probably do not affect the native configuration of the products. Amino-terminal amino acids of human Dcate, D EGTA and D-dimer are identical i.e. val, asx and ser. in the ratgly, asx and ser were found. E 1% for rat Dcate=17-8 for rat D EGTA=16.2 and for rat D- dimer=l8.3. for the corresponding human fragments, these values were all 20.0 ± 0.2.

Nomenclature Abstract for Bacillus fastidiosus den Dooren de Jong 1929 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
George M Garrity
Keyword(s):  
Bacillus Fastidiosus

Simple and Rapid Purification of Histatins Using Hydroxyapatite Chromatography in Denaturalized Conditions.

2000 ◽  
Vol 42 (2) ◽  
pp. 160-165 ◽  
Author(s):  
Takashi Kanehira ◽  
Hiroshi Tani ◽  
Pao-Li Wang ◽  
Kiyoshi Ohura ◽  
Yoshinori Kuboki
Keyword(s):  
Rapid Purification

scholarly journals A novel procedure for the rapid purification of plastocyanin from pea (Pisum sativum) leaves

1981 ◽  
Vol 193 (1) ◽  
pp. 375-378 ◽  
Author(s):  
A R Ashton ◽  
L E Anderson
Keyword(s):  
Ionic Strength ◽  
Pisum Sativum ◽  
Deae Cellulose ◽  
High Concentrations ◽  
Rapid Purification ◽  
Low Ionic Strength

Plastocyanin is soluble at high concentrations (greater than 3 M) of (NH4)2SO4 but under these conditions will adsorb tightly to unsubstituted Sepharose beads. This observation was utilized to purify plastocyanin from pea (Pisum sativum) in two chromatographic steps. Sepharose-bound plastocyanin was eluted with low-ionic-strength buffer and subsequently purified to homogeneity by DEAE-cellulose chromatography.

scholarly journals A simple and rapid purification method of condensed tannins from several young fruits.

1981 ◽  
Vol 45 (8) ◽  
pp. 1885-1887 ◽  
Author(s):  
Tomoaki MATSUO ◽  
Saburo ITOO
Keyword(s):  
Condensed Tannins ◽  
Purification Method ◽  
Rapid Purification

scholarly journals Rapid purification of phospholipase A2 from Crotalus adamanteus venom by affinity chromatography

1975 ◽  
Vol 250 (16) ◽  
pp. 6564-6566
Author(s):  
CO Rock ◽  
F Snyder
Keyword(s):  
Phospholipase A2 ◽  
Affinity Chromatography ◽  
Rapid Purification

scholarly journals Ribonuclease inhibitor from human placenta: rapid purification and assay.

1979 ◽  
Vol 254 (24) ◽  
pp. 12484-12487
Author(s):  
P. Blackburn
Keyword(s):  
Human Placenta ◽  
Ribonuclease Inhibitor ◽  
Rapid Purification
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