A novel procedure for the rapid purification of plastocyanin from pea (Pisum sativum) leaves
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Plastocyanin is soluble at high concentrations (greater than 3 M) of (NH4)2SO4 but under these conditions will adsorb tightly to unsubstituted Sepharose beads. This observation was utilized to purify plastocyanin from pea (Pisum sativum) in two chromatographic steps. Sepharose-bound plastocyanin was eluted with low-ionic-strength buffer and subsequently purified to homogeneity by DEAE-cellulose chromatography.
2013 ◽
Vol 690-693
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pp. 1286-1291
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2021 ◽
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1959 ◽
Vol 03
(04)
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pp. 640-653
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1974 ◽
Vol 29
(9-10)
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pp. 496-505
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1988 ◽
Vol 46
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pp. 176-177
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