Effects of basic fibroblast growth factor and ganglioside GM1 on neuronal survival in primary cultures and on eight-arm radial maze task in adult rats following partial fimbria transections

1996 ◽  
Vol 353 (3) ◽  
pp. 342-348 ◽  
Author(s):  
A. Iwashita ◽  
H. Hisajima ◽  
Y. Notsu ◽  
M. Okuhara
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Radial Maze ◽  
Primary Cultures ◽  
Fibroblast Growth ◽  
Ganglioside Gm1 ◽  
Maze Task ◽  
Adult Rats ◽  
Radial Maze Task

scholarly journals Folliculostellate Cells Determine the Susceptibility of Lactotropes to Estradiol’s Mitogenic Action

Endocrinology ◽  
2004 ◽  
Vol 145 (3) ◽  
pp. 1473-1480 ◽  
Author(s):  
Souichi Oomizu ◽  
Kirti Chaturvedi ◽  
Dipak K. Sarkar
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Primary Cultures ◽  
Human Populations ◽  
Fibroblast Growth ◽  
Estradiol Treatment ◽  
Sd Rats ◽  
Mitogenic Action ◽  
F344 Rats

Abstract Estradiol is known to increase lactotropic cell proliferation, but estradiol susceptibility varies among human populations and among various strains of rats. We had reported that folliculostellate (FS) cells regulate estradiol’s mitogenic action on lactotropes; therefore, we studied their role in determining the susceptibility to estradiol in a high estradiol-responsive rat strain, Fischer 344 (F344), and in a low-responsive strain, Sprague Dawley (SD). Determination of total S-100-positive FS cells in the pituitary revealed that F344 rats have significantly more FS cells than do SD rats. Estradiol treatment did not change the number of FS cells in both F344 and SD rats. When cotransplanted with F344 pituitaries under the kidney capsule or cocultured with F344-derived lactotropes in vitro, FS cells derived from F344 rats increased estradiol’s mitogenic action. They also increased estradiol’s mitogenic action on SD-derived lactotropes in primary cultures. However, SD-derived FS cells failed to increase estrogen’s action on F344- or SD-derived lactotropes. The levels of basic fibroblast growth factor production and secretion by TGF-β3 and estradiol were much higher in F344-derived FS cells than in SD-derived FS cells. However, the lactotropes’ growth response to basic fibroblast growth factor was similar in both strains. These data suggest that cell-cell interaction between FS cells and lactotropes regulates estradiol’s mitogenic action on lactotropes and also determines lactotrope susceptibility to the steroid.

scholarly journals Basic Fibroblast Growth Factor Protects against Excitotoxicity and Chemical Hypoxia in Both Neonatal and Adult Rats

1995 ◽  
Vol 15 (4) ◽  
pp. 619-623 ◽  
Author(s):  
Pamela B. Kirschner ◽  
Ross Henshaw ◽  
Jens Weise ◽  
Vladimir Trubetskoy ◽  
Seth Finklestein ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Neuroprotective Effect ◽  
Systemic Administration ◽  
Fibroblast Growth ◽  
Neonatal Rats ◽  
Adult Rats ◽  
Chemical Hypoxia ◽  
Intrastriatal Injection

Basic fibroblast growth factor (bFGF) is a polypeptide growth factor that promotes neuronal survival. We recently found that systemic administration of bFGF protects against both excitotoxicity and hypoxia–ischemia in neonatal animals. In the present study, we examined whether systemically administered bFGF could prevent neuronal death induced by intrastriatal injection of N-methyl-d-aspartate (NMDA) or chemical hypoxia induced by intrastriatal injection of malonate in adult rats and 1-methyl-4-phenylpyridinium (MPP+) in neonatal rats. Systemic administration of bFGF (100 μg/kg) for three doses both before and after intrastriatal injection of either NMDA or malonate in adult rats produced a significant neuroprotective effect. In neonatal rats, bFGF produced dose-dependent significant neuroprotective effects against MPP+ neurotoxicity, with a maximal protection of ∼50% seen with either a single dose of bFGF of 300 μg/kg or three doses of 100 μg/kg. These results show that systemic administration of bFGF is effective in preventing neuronal injury under circumstances in which the blood–brain barrier may be compromised, raising the possibility that this strategy could be effective in stroke.

Electron Microscopic Immunolocalization of Basic Fibroblast Growth Factor-Like Molecules in Capillary Endothelial Cells

1998 ◽  
Vol 4 (S2) ◽  
pp. 1100-1101
Author(s):  
Ranan Gullhan Aktas ◽  
Robert J. Kayton
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Immunogold Labeling ◽  
Fibroblast Growth ◽  
Electron Microscopic ◽  
Adult Rats

Basic fibroblast growth factor (bFGF) is a potent angiogenic polypeptide. It promotes angiogenesis in vivo and in vitro by stimulating migration, proliferation and proteolytic activity of endothelial cells. Whereas several effects of exogenous bFGF on endothelial cells have been described, it has remained unclear how endogenous bFGF produced by vascular endothelial cells regulate angiogenesis.To further investigate functional implications of the distribution of bFGF, we undertook the present study. Our aims were (i) to identify the specific location of bFGF in endothelial cells using electron microscopy immunogold labeling technique (ii) to determine the distribution of bFGF in capillaries of different types of tissues.Tissue samples from sciatic nerve, hippocampus, adrenal gland and kidney of normal adult rats were fixed in 4% paraformaldehyde/1 to 5% glutaraldehyde and embedded in Spurr's resin. Ultrathin sections were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-ZymoGenetics) specific for bFGF using a two-step immunogold labeling method.

Basic fibroblast growth factor and growth factor receptor gene expression in 85% O2-exposed rat lung

1995 ◽  
Vol 268 (3) ◽  
pp. L455-L464 ◽  
Author(s):  
S. Buch ◽  
R. N. Han ◽  
J. Liu ◽  
A. Moore ◽  
J. D. Edelson ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Receptor Gene ◽  
Type I ◽  
Type Ii ◽  
Fibroblast Growth ◽  
Rat Lung ◽  
Adult Rats ◽  
Type Ii Pneumocyte

Lungs exposed to elevated O2 concentrations suffer an initial loss of type I pneumocytes, followed by a reparative type II pneumocyte hyperplasia. We hypothesized that type II pneumocyte hyperplasia after exposure of young adult rats to 85% O2 in vivo would be temporally related to 1) an increased concentration of intrapulmonary basic fibroblast growth factor (bFGF), a potent stimulator of type II pneumocyte DNA synthesis in vitro, and 2) an upregulation of pneumocyte receptors for bFGF (FGF-R). Increased rat lung bFGF mRNA, relative to air-exposed control animals, was observed at 4 days of exposure, with no increase at days 6 and 14 of exposure. Parallel changes were observed with bFGF receptor (flg) mRNA. Nuclear runoff assays confirmed increased transcription of both bFGF and flg genes in response to 85% O2, whereas increased translation at 6 days of exposure was confirmed by protein immunoanalysis. Immunohistochemistry demonstrated a broad distribution of bFGF throughout the lung, including the alveolar epithelium, which increased after 6 and 14 days of exposure to 85% O2. Our findings are compatible with a role for bFGF in O2-mediated pneumocyte hyperplasia.

The effects of basic fibroblast growth factor in an animal model of acute mechanically induced right ventricular hypertrophy

2012 ◽  
Vol 22 (4) ◽  
pp. 436-442
Author(s):  
Vladimiro L. Vida ◽  
Arben Dedja ◽  
Elisabetta Faggin ◽  
Simone Speggiorin ◽  
Massimo A. Padalino ◽  
...  
Keyword(s):  
Growth Factor ◽  
Right Ventricular ◽  
Continuous Infusion ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Ventricular Myocardium ◽  
Fibroblast Growth ◽  
Adult Rats ◽  
The Right ◽  
Basic Fibroblastic Growth Factor

AbstractObjectiveTo evaluate the effect of a continuous infusion of basic fibroblast growth factor on the adaptive potential of the right ventricular myocardium after 30 days of mechanically induced overload in rats.Materials and methodsWe banded the pulmonary trunk, so as to increase the systolic workload of the right ventricle, in six Lewis/HanHsd rats at the age of 11 weeks, using six adult rats as controls. The six adult rats were also banded and received an additional continuous infusion of basic fibroblastic growth factor, using six rats with a continuous infusion of basic fibroblastic growth factor only as controls. We analysed the functional adaptation and structural changes of the right ventricular myocardium, blood vessels, and interstitial tissue 30 days after the increased afterload.ResultsThe pulmonary artery banding induced an increase in the right ventricular free wall thickness of banded rats when compared with controls, which was mainly justified by an increase in cardiomyocyte area and in the percentage of extracellular fibrosis. The infusion of basic fibroblastic growth factor promotes a more extensive capillary network in banded rats (p < 0.001), which modulates the compensatory response of the right ventricle, promoting the hypertrophy of contractile elements and limiting the areas in which fibrosis develops (p < 0.001).ConclusionsThe subcutaneous infusion with osmotic pumps was a valid and reproducible method of delivering basic fibroblast growth factor to heart tissue. This infusion contributed to better preserve the right ventricular capillary network, hampering the development of interstitial fibrosis.

Ultrastructural Distribution of Basic Fibroblast Growth Factor-Like Molecules in Peripheral Nerves

1998 ◽  
Vol 4 (S2) ◽  
pp. 1102-1103
Author(s):  
Robert J. Kayton ◽  
Ranan Gulhan Aktas
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Peripheral Nerves ◽  
Fibroblast Growth ◽  
Adult Rats ◽  
Ultrathin Sections ◽  
Few Data ◽  
Labeling Method

Basic Fibroblast Growth Factor (bFGF) is a multifunctional polypeptide which has been shown to play a pivotal role in the survival and differentiation of nerve cells. Several trophic and non-trophic functions of this protein have been suggested in peripheral nerves. In spite of ample information about the distribution and effects of bFGF in central nervous system, few data are available concerning the localization of this protein in peripheral nerves. In view of the role of bFGF in regulation of trophic and non-trophic functions, we particularly focused on the presence and precise location of bFGF in peripheral nerves at the electron microscope level.Spurr's resin embedded ultrathin sections from adult rats’ sural nerves were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-Zymogenetics) specific for bFGF using two-step immunogold labeling method. Control samples were treated with either an equivalent volume of blocking solution (omitting the primary antibody) or an irrelevant antibody (Factor VIII, VGF, anti-histamine, anti-fibroblast 5B5).

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