scholarly journals Small blood vessel involvement in diabetes mellitus: Light microscope study of specimens obtained by ear lobe biopsy

Diabetologia ◽  
1965 ◽  
Vol 1 (2) ◽  
pp. 109-115 ◽  
Author(s):  
Pieri Antonio ◽  
Paolo T. Scarpelli ◽  
Michelangelo Rizzo
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.


1957 ◽  
Vol 3 (2) ◽  
pp. 193-202 ◽  
Author(s):  
Alan R. Muir

Prenatal and postnatal cardiac muscle from rabbits has been studied by electron microscopy, after osmium fixation and methacrylate embedding. The observations showed that 1. Cell membranes divide the muscle into cellular units from the youngest embryo which was studied (9½ days after coitus) until the adult state. 2. The embryonic muscle cells contain only one nucleus, whereas the adult cell may be multinucleated. 3. At all stages of development, wherever a myofibrillar axis crosses a cellular boundary, the myofilaments are interrupted by an intercalated disc. 4. With age, increase in size and complexity of the discs render them recognisable by the light microscope.


Blood ◽  
1960 ◽  
Vol 15 (4) ◽  
pp. 480-490 ◽  
Author(s):  
JAMES C. HAMPTON

Abstract Evidence that erythrocytes are phagocytized and dismantled by hepatic parenchymal cells in the newborn rabbit is presented. It is concluded that in these cells iron is recovered from disintegrating erythrocytes, synthesized into ferritin and released into the hepatic cell cytoplasm and into the biliary passages. These conclusions are based upon observations on the distribution of material giving the Prussian blue reaction in sections of liver as revealed by the light microscope and upon electron microscopic images of particles displaying the size, density and configuration of the ferritin molecule.


1993 ◽  
Vol 206 (1) ◽  
pp. 16-26 ◽  
Author(s):  
John W. Breneman ◽  
Peter Yau ◽  
Raymond L. Teplitz ◽  
E.Morton Bradbury

Development ◽  
1965 ◽  
Vol 13 (1) ◽  
pp. 73-81
Author(s):  
Suzanne L. Ullmann

In many insect eggs, including those of the Diptera, deeply staining granules, rich in RNA, occur in the posterior polar plasm and during ontogeny become enclosed within the pole cells. The structure and fate of these cells, which generally give rise to the primordial germ cells, and their inclusions have excited interest for over half a century (Hegner, 1908; Huettner, 1923; Rabinowitz, 1941; Poulson, 1947; Counce, 1963; Mahowald, 1962), yet numerous questions concerning them remain unsettled or controversial to this day. For instance, the dual fate of the pole cells in Drosophila, the genus which has been most extensively studied, is still debated (Poulson & Waterhouse, 1960; Hathaway & Selman, 1961). Recently, Counce (1963), in a light-microscope study, has described the developmental morphology of the polar granules in several species of Drosophila embryos; while Mahowald (1962) has succeeded in identifying them in D. melanogaster at the ultra-structural level.


2012 ◽  
Vol 19 (1) ◽  
Author(s):  
Ghada Abdel-Hamid ◽  
Rana El-Beshbishy ◽  
Afrah Al-Awa

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