Non-radioactive HighSens detection of nucleic acids. A novel highly sensitive DNA labeling and detection system based on digoxigenin: anti-digoxigenin enzyme-linked immuno-sorbent assay (ELISA) [1]

1988 ◽  
Vol 330 (4-5) ◽  
pp. 305-306 ◽  
Author(s):  
R. Seibl ◽  
J. Höltke ◽  
J. Burg ◽  
K. Mühlegger ◽  
R. Mattes ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Detection System ◽  
Dna Labeling ◽  
Highly Sensitive

Non-radioactive Labeling and Detection of Nucleic Acids. I. A Novel DNA Labeling and Detection System Based on Digoxigenin: Anti-Digoxigenin ELISA Principle (Digoxigenin System)

1990 ◽  
Vol 371 (2) ◽  
pp. 917-928 ◽  
Author(s):  
Christoph KESSLER ◽  
Hans-Joachim HÖLTKE ◽  
Rudolf SEIBL ◽  
Josef BURG ◽  
Klaus MÜHLEGGER
Keyword(s):  
Nucleic Acids ◽  
Detection System ◽  
Dna Labeling

Application strategies of peptide nucleic acids toward electrochemical nucleic acid sensors

The Analyst ◽  
2021 ◽  
Author(s):  
Qingteng Lai ◽  
Wei Chen ◽  
Yanke Zhang ◽  
Zheng-Chun Liu
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Dna Probes ◽  
Highly Sensitive ◽  
Increased Sensitivity ◽  
Acid Sensors

Peptide nucleic acids (PNAs) have attracted tremendous interest in the fabrication of highly sensitive electrochemical nucleic acid biosensor due to their higher stability and increased sensitivity than common DNA probes....

scholarly journals COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR

1996 ◽  
Vol 42 (12) ◽  
pp. 1915-1923 ◽  
Author(s):  
N DiDomenico ◽  
H Link ◽  
R Knobel ◽  
T Caratsch ◽  
W Weschler ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Internal Control ◽  
Detection System ◽  
Dna Amplification ◽  
Cross Reactivity ◽  
Diagnostic Pcr ◽  
Thermal Cycler ◽  
Paramagnetic Microparticles ◽  
Rna And Dna ◽  
Single Reaction

Abstract The COBAS AMPLICOR system automates amplification and detection of target nucleic acids, making diagnostic PCR routine for a variety of infectious diseases. The system contains a single thermal cycler with two independently regulated heating/cooling blocks, an incubator, a magnetic particle washer, a pipettor, and a photometer. Amplified products are captured on oligonucleotide-coated paramagnetic microparticles and detected with use of an avidin-horseradish peroxidase (HRP) conjugate. Concentrated solutions of amplicon or HRP were pipetted without detectable carryover. Amplified DNA was detected with an intraassay CV of < 4.5%; the combined intraassay CV for amplification and detection was < 15%. No cross-reactivity was observed when three different target nucleic acids were amplified in a single reaction and detected with three target-specific capture probes. The initial COBAS AMPLICOR menu includes qualitative tests for diagnosing infections with Chlamydia trachomatis, Neisseria gonorrhoeae, Mycobacterium tuberculosis, and hepatitis C virus. All tests include an optional Internal Control to provide assurance that specimens are successfully amplified and detected.

A sensitive SERS assay for detecting proteins and nucleic acids using a triple-helix molecular switch for cascade signal amplification

2014 ◽  
Vol 50 (66) ◽  
pp. 9409-9412 ◽  
Author(s):  
Sujuan Ye ◽  
Yanying Wu ◽  
Wen Zhang ◽  
Na Li ◽  
Bo Tang
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Triple Helix ◽  
Detection System ◽  
Molecular Switch ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Multiple Cycle ◽  
Enhanced Raman Scattering

A sensitive surface-enhanced Raman scattering (SERS) detection system is developed for proteins and nucleic acids based on a triple-helix molecular switch for multiple cycle signal amplification.

scholarly journals Highly sensitive dual mode electrochemical platform for microRNA detection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pawan Jolly ◽  
Marina R. Batistuti ◽  
Anna Miodek ◽  
Pavel Zhurauski ◽  
Marcelo Mulato ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Dual Mode ◽  
Electrode Surfaces ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Detection Mode ◽  
Amplification Strategy

Abstract MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of microRNAs. The platform was developed using peptide nucleic acids as probes on gold electrode surfaces to capture target miRNAs. A simple amplification strategy using gold nanoparticles has been employed exploiting the inherent charges of the nucleic acids. Electrochemical impedance spectroscopy was used to monitor the changes in capacitance upon any binding event, without the need for any redox markers. By using thiolated ferrocene, a complementary detection mode on the same sensor was developed where the increasing peaks of ferrocene were recorded using square wave voltammetry with increasing miRNA concentration. This dual-mode approach allows detection of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatched target miRNA sequences. The electrochemical platform developed can be easily expanded to other miRNA/DNA detection along with the development of microarray platforms.

Highly Sensitive, Selective, and Rapid Detection of miRNA-21 using an RCA/G-Quadruplex/QnDESA Probing System

2021 ◽  
Author(s):  
Young Jun Seo ◽  
Tasnima Alam Asa ◽  
Ravi Kumara Guralamatta Siddappa
Keyword(s):  
Rapid Detection ◽  
Detection System ◽  
Single Step ◽  
Highly Sensitive ◽  
G Quadruplex

In this study we developed a very simple and rapid miRNA 21 detection system using a novel quinolinium diethylamino salicylaldehyde (QnDESA) probe for sensing the 22AG hybrid G-quadruplex with single-step...

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