Isolation and biochemical analysis of a temperature-sensitive scarlet eye color mutant of Drosophila melanogaster

1979 ◽  
Vol 17 (1-2) ◽  
pp. 149-158 ◽  
Author(s):  
A. J. Howells
Keyword(s):  
Drosophila Melanogaster ◽  
Biochemical Analysis ◽  
Temperature Sensitive ◽  
Eye Color ◽  
Color Mutant

A biochemical study of the scarlet eye-color mutant of Drosophila melanogaster

1975 ◽  
Vol 13 (3-4) ◽  
pp. 273-282 ◽  
Author(s):  
A. J. Howells ◽  
Rosemary L. Ryall
Keyword(s):  
Drosophila Melanogaster ◽  
Biochemical Study ◽  
Eye Color ◽  
Color Mutant

scholarly journals Genetic analysis of the enhancer of zeste locus and its role in gene regulation in Drosophila melanogaster.

Genetics ◽  
1990 ◽  
Vol 126 (1) ◽  
pp. 185-199 ◽  
Author(s):  
R S Jones ◽  
W M Gelbart
Keyword(s):  
Gene Regulation ◽  
Drosophila Melanogaster ◽  
Ectopic Expression ◽  
Polycomb Group ◽  
Temperature Sensitive ◽  
Loss Of Function ◽  
Eye Color ◽  
Polycomb Group Genes ◽  
Enhancer Of Zeste ◽  
Gene Complexes

Abstract The Enhancer of zeste [E(z)] locus of Drosophila melanogaster is implicated in multiple examples of gene regulation during development. First identified as dominant gain-of-function modifiers of the zeste1-white (z-w) interaction, mutant E(z) alleles also produce homeotic transformations. Reduction of E(z)+ activity leads to both suppression of the z-w interaction and ectopic expression of segment identity genes of the Antennapedia and bithorax gene complexes. This latter effect defines E(z) as a member of the Polycomb-group of genes. Analysis of E(z)S2, a temperature-sensitive E(z) allele, reveals that both maternally and zygotically produced E(z)+ activity is required to correctly regulate the segment identity genes during embryonic and imaginal development. As has been shown for other Polycomb-group genes, E(z)+ is required not to initiate the pattern of these genes, but rather to maintain their repressed state. We propose that the E(z) loss-of-function eye color and homeotic phenotypes may both be due to gene derepression, and that the E(z)+ product may be a general repressing factor required for both examples of negative gene regulation.

Über das Auftreten von Tetrahydrobiopterin und Riboflavin in den Malpighischen Gefäßen von Drosophila melanogaster und deren Mutante brown

1969 ◽  
Vol 24 (1) ◽  
pp. 123-127
Author(s):  
Dieter Eichelberg
Keyword(s):  
Drosophila Melanogaster ◽  
Uric Acid ◽  
Malpighian Tubules ◽  
Wild Type ◽  
Strong Reduction ◽  
Stages Of Development ◽  
Eye Color ◽  
Color Mutant

This paper is concerned with the different occurrence of tetrahydrobiopterin (THB) and riboflavine in the Malpighian tubules (MT) of the wild-type and of the eye color mutant brown (bw) of Drosophila melanogaster during different stages of development and life. In the MT of the mutant brown there is a strong reduction of THB and riboflavine contents in proportion to the amounts of the MT of the wild-type. Furthermore, in the mutant brown the THB, similar like isoxanthopterin and uric acid, disappears out of the MT soon after hatching. The results are discussed with regard to biogenetic coherences.

A Temperature Sensitive Eye-Color Mutant in a Mosquito

1974 ◽  
Vol 65 (6) ◽  
pp. 359-361
Author(s):  
RICHARD H. BAKER ◽  
RICHARD K. SAKAI
Keyword(s):  
Temperature Sensitive ◽  
Eye Color ◽  
Color Mutant

scholarly journals A GENETIC AND BIOCHEMICAL ANALYSIS OF THE TEMPERATURE SENSITIVE, NORMAL-WINGED ALLELES OF THE RUDIMENTARY LOCUS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1977 ◽  
Vol 86 (4) ◽  
pp. 765-777
Author(s):  
Darrel R Falk ◽  
Gary McCaughin ◽  
Thomas Cogley
Keyword(s):  
Drosophila Melanogaster ◽  
High Temperatures ◽  
Temperature Sensitivity ◽  
Biochemical Analysis ◽  
Temperature Sensitive ◽  
Aspartate Transcarbamylase ◽  
Biochemical Characteristics ◽  
Pyrimidine Synthesis ◽  
Leaky Mutants

ABSTRACT The genetic and biochemical characteristics of a particular class of mutants at the rudimentary locus are described. The mutants are pyrimidine auxotrophs, like classical rudimentary alleles, but they are unique in that they do not alter the size or shape of the wing (Falk and Nash 1974b). Aspartate transcarbamylase and dihydroorotase activities have been measured in seven different normal-winged mutants, and the results indicate that these strains are enzymologically "leaky" mutants. Previous studies have shown that three genetic functions (corresponding to the first three enzymes of pyrimidine synthesis) are associated with the rudimentary locus. Four of the seven mutants appear to affect all three of these functions. Each of the four is temperature sensitive, and a biochemical analysis of the temperature sensitivity of one of these mutants, (r)pyr1-3, suggests that a process affecting the synthesis or assembly of these enzymes is altered at high temperatures.

Whole-Genome Effects of Ethyl Methanesulfonate-Induced Mutation on Nine Quantitative Traits in Outbred Drosophila melanogaster

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1257-1265 ◽  
Author(s):  
Hsiao-Pei Yang ◽  
Ana Y Tanikawa ◽  
Wayne A Van Voorhies ◽  
Joana C Silva ◽  
Alexey S Kondrashov
Keyword(s):  
Drosophila Melanogaster ◽  
Quantitative Traits ◽  
Spontaneous Mutation ◽  
Developmental Time ◽  
Ethyl Methanesulfonate ◽  
Induced Mutations ◽  
Mean Values ◽  
Eye Color ◽  
Recessive Mutations ◽  
The Mean

Abstract We induced mutations in Drosophila melanogaster males by treating them with 21.2 mm ethyl methanesulfonate (EMS). Nine quantitative traits (developmental time, viability, fecundity, longevity, metabolic rate, motility, body weight, and abdominal and sternopleural bristle numbers) were measured in outbred heterozygous F3 (viability) or F2 (all other traits) offspring from the treated males. The mean values of the first four traits, which are all directly related to the life history, were substantially affected by EMS mutagenesis: the developmental time increased while viability, fecundity, and longevity declined. In contrast, the mean values of the other five traits were not significantly affected. Rates of recessive X-linked lethals and of recessive mutations at several loci affecting eye color imply that our EMS treatment was equivalent to ∼100 generations of spontaneous mutation. If so, our data imply that one generation of spontaneous mutation increases the developmental time by 0.09% at 20° and by 0.04% at 25°, and reduces viability under harsh conditions, fecundity, and longevity by 1.35, 0.21, and 0.08%, respectively. Comparison of flies with none, one, and two grandfathers (or greatgrandfathers, in the case of viability) treated with EMS did not reveal any significant epistasis among the induced mutations.

scholarly journals THE MECHANISM OF A BROODING EFFECT ASSOCIATED WITH SEGREGATION DISTORTION IN DROSOPHILA MELANOGASTER

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 619-631
Author(s):  
D L Hartl
Keyword(s):  
Drosophila Melanogaster ◽  
Segregation Distortion ◽  
Mature Sperm ◽  
Temperature Sensitive ◽  
Short Time

ABSTRACT The recovery of the SD chromosome from a heterozygous SD male increases with brood. This is independent of the age of the female, occurs during the time the sperm are stored in the females, disappears when the segregation distortion is suppressed, and is temperature-sensitive-temperature shocks above or below 25°C applied to the mature sperm both tend to accelerate the increase in the recovery of SD. All this suggests the existence of a class of sperm affected by SD in which the sperm are able to fertilize eggs for a short time following ejaculation but become dysfunctional thereafter.

Die fluoreszierenden Stoffe aus den Malpighischen-Gefäßen der Wildform und verschiedener Augenfarbenmutanten von Drosophila melanogaster

1968 ◽  
Vol 23 (3) ◽  
pp. 376-386 ◽  
Author(s):  
Armin Wessing ◽  
Dieter Eichelberg
Keyword(s):  
Drosophila Melanogaster ◽  
Malpighian Tubules ◽  
Wild Type ◽  
Eye Color ◽  
Eye Color Mutants

The Malpighian tubules of Drosophila melanogaster accumulate a great number of substances, many of which fluoresce. This paper is concerned with the identification of these substances by chromatography and their location by fluorescentmicroscopy (fig. 4, 5). It appears that they mainly belong to the following three groups: Pteridines, tryptophane and some of its metabolites, and riboflavine (tab. 1).The pattern of fluorescent substances of the eye color mutants cn, v, se, st, bw, ry, and w vary significantly. The patterns of these mutants are compared and discussed with that of the wild-type.

Cytogenetic Analyses of PSL1 Mutant, a Novel Low‐Temperature‐Sensitive Purple‐Striped Leaf Color Mutant in Wheat

Crop Science ◽  
2018 ◽  
Vol 58 (5) ◽  
pp. 1919-1931 ◽  
Author(s):  
Cong Liu ◽  
Narong Shi ◽  
Huiyu Wu ◽  
Xuyao An ◽  
Jinjuan Zheng ◽  
...  
Keyword(s):  
Low Temperature ◽  
Temperature Sensitive ◽  
Leaf Color ◽  
Color Mutant ◽  
Cytogenetic Analyses ◽  
Leaf Color Mutant
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