Spectroscopic and photophysical investigations on the nature of localization of rhodamine-123 and its dibromo derivative in different cell lines

1996 ◽  
Vol 6 (4) ◽  
pp. 209-219 ◽  
Author(s):  
Luc Villeneuve ◽  
Prabir Pal ◽  
Gilles Durocher ◽  
David Migneault ◽  
Denis Girard ◽  
...  
Keyword(s):  
Cell Lines ◽  
Rhodamine 123 ◽  
Dibromo Derivative

UV or X-Irradiation Increases the Cytoplasmic Accumulation of Rhodamine 123 in Various Cancer Cell Lines

2003 ◽  
Vol 179 (8) ◽  
pp. 564-570 ◽  
Author(s):  
Ingrid Elena Dumitriu ◽  
Franz Roedel ◽  
Thomas D. Beyer ◽  
Udo S. Gaipl ◽  
Joachim R. Kalden ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Rhodamine 123 ◽  
X Irradiation ◽  
Cytoplasmic Accumulation

In vitro PHOTOSENSITIZING PROPERTIES OF RHODAMINE 123 ON DIFFERENT HUMAN TUMOR CELL LINES

1988 ◽  
Vol 48 (3) ◽  
pp. 311-314 ◽  
Author(s):  
Elsa Melloni ◽  
Teresa Dasdia ◽  
Giannino Fava ◽  
Emilio Rocca ◽  
Franco Zunino ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Human Tumor ◽  
Rhodamine 123 ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines

Influence of sequential exposure to R-verapamil or B8509-035 on rhodamine 123 accumulation in human lymphoblastoid cell lines

1993 ◽  
Vol 32 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Elke Roller ◽  
Britta Klumpp ◽  
Jessica Krause ◽  
Michel Eichelbaum ◽  
Kurt Schumacher
Keyword(s):  
Cell Lines ◽  
Rhodamine 123 ◽  
Lymphoblastoid Cell Lines ◽  
Human Lymphoblastoid Cell ◽  
Human Lymphoblastoid Cell Lines

Anti-Leukemic Activity of Mitochondrially-Directed Asymmetrical 2-2'-Binaphthoquinones.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4799-4799
Author(s):  
Rebecca Ricklis ◽  
Milena Vuica-Ross ◽  
Robert Brodsky ◽  
Ed Sausville ◽  
Chi Van Dang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mitochondrial Membrane ◽  
Conflicts Of Interest ◽  
Lymphoblastic Leukemia ◽  
Leukemia Cell ◽  
Xenograft Model ◽  
Leukemic Cells ◽  
Rhodamine 123 ◽  
A Genome ◽  
Ic50 Values

Abstract Abstract 4799 Introduction Binaphthoquinones are unique molecules consisting of two linked naphthoquinone units. Previously, we regiospecifically synthesized a series of asymmetrical 2,2'-binaphthoquinones, which possess HIV integrase inhibitory activity. Potent activity against non-HIV-infected CEM-T4 acute lymphoblastic leukemia cells prompted us to investigate other cytotoxic mechanisms of these compounds. A genome-wide yeast screen uncovered that mitochondrial-related genes are required for sensitivity and resistance to these agents. Furthermore, by generating reactive oxygen species (ROS), biquinones halted yeast growth which was rescued by addition of N-acetylcysteine. Therefore, as part of our efforts to identify new compounds with anti-leukemic activity, we hypothesized that 2,2'-binaphthoquinones would be able to kill leukemic cells by interference with mitochondrial function. The majority of studies on antineoplastic effects of quinones have focused on benzoquinones, anthraquinones or monomeric naphthoquinones. However, nothing is published on the anti-leukemic action of asymmetrical 2,2'-binaphthoquinones, in which two different mononaphthoquinones are attached at the quinone double bond. Biquinones possess four carbonyl groups that have the potential to generate a greater number of ROS per one mole of quinone, and thereby cause more effective oxidative stress, than their monoquinone counterparts. In addition, the potential differences in cytotoxicities between monomeric and dimeric naphthoquinones result from many parameters, including the number of units, planarity, and the specific nature and pattern of aromatic functional groups. Materials and Methods Seventeen of our biquinones were dissolved in DMSO and incubated with four leukemia cell lines grown under standard conditions. All cultures were maintained with a constant DMSO concentration. A standard MTT cell proliferation assay was used to identify IC50 values. MV411 cells were incubated for 42-49 hours (2 nights) with two of the most potent compounds, biquinone #7 (BiQ7) and biquinone #10 (BiQ10), and they were assayed and analyzed by flow cytometry for loss of mitochondrial membrane potential (via rhodamine 123 staining), as well as a variety of apoptosis markers such as exposed phosphatidylserine, activated caspases, and sub-2n DNA increases. Simultaneously, preliminary toxicology experiments have been performed on mice. Results The IC50 values for both compounds were less than 5 micromolar (μM) against all four cell lines. For subsequent experiments, the cells were treated with either 5 μM BiQ7 or 5 μM BiQ10. A loss of mitochondrial membrane electrochemical potential, as expressed and evidenced by substantial decrease in fluorescence intensity of Rhodamine 123, was observed in 98-100% of the treated cells compared to control (5%). Loss of plasma membrane phosphatidylserine asymmetry was observed via Annexin V- PE (with 7AAD) staining in 94-95% of the treated cells and in only 18% of the control cells. Furthermore, the treated cells showed increases in staining for activated caspases 3 & 7 (BiQs 96-98%; control 10%). Finally, the cell cycle analyses via propidium iodide showed increases in sub-2n DNA in the treated cells compared to the control (control 12%; BiQ7 34%; and BiQ10 54%). Importantly, the preliminary toxicology results in mice suggest selectivity for neoplastic cells since no cytopenia or obvious end organ toxicity was observed with injection of 600 μM binaphthoquinones intraperitoneally daily for three weeks. Conclusion Our study shows that this new class of compounds possesses promising in vitro anti-leukemic effects by targeting mitochondrial membrane permeabilization, which occurs early in the apoptotic program and is located downstream of most identified chemotherapy resistance mechanisms in hematologic malignancies. In vivo experiments in xenograft model and ex vivo experiments on primary human cells are planned. Disclosures: No relevant conflicts of interest to declare.

Specificity of doxorubicin versus rhodamine-123 in assessing P-glycoprotein functionality in the LLC-PK1, LLC-PK1:MDR1 and Caco-2 cell lines

2000 ◽  
Vol 11 (3) ◽  
pp. 207-214 ◽  
Author(s):  
Inez C.J van der Sandt ◽  
Margret C.M Blom-Roosemalen ◽  
Albertus G de Boer ◽  
Douwe D Breimer
Keyword(s):  
Cell Lines ◽  
Rhodamine 123 ◽  
P Glycoprotein

scholarly journals Rhodamine 123 fluorescence of immortal hybridoma cell lines as a function of glucose concentration

Cytometry ◽  
1993 ◽  
Vol 14 (1) ◽  
pp. 70-73 ◽  
Author(s):  
Nicole Borth ◽  
Gerda Kral ◽  
Hermann Katinger
Keyword(s):  
Cell Lines ◽  
Glucose Concentration ◽  
Rhodamine 123 ◽  
Hybridoma Cell Lines

Intracellular distribution of a platinum-rhodamine 123 complex in cis-platinum sensitive and resistant human squamous carcinoma cell lines

1986 ◽  
Vol 35 (19) ◽  
pp. 3365-3369 ◽  
Author(s):  
Beverly A. Teicher ◽  
Sylvia A. Holden ◽  
John L. Jacobs ◽  
Michael J. Abrams ◽  
Alun G. Jones
Keyword(s):  
Cell Lines ◽  
Carcinoma Cell ◽  
Squamous Carcinoma ◽  
Intracellular Distribution ◽  
Rhodamine 123 ◽  
Platinum Sensitive ◽  
Carcinoma Cell Lines ◽  
Squamous Carcinoma Cell ◽  
In Cis

(EB) Virus: Latency and Expression in Transplanted and Cultured Human Cells

1971 ◽  
Vol 29 ◽  
pp. 368-369
Author(s):  
B. G. Uzman ◽  
M. M. Kasac ◽  
H. Saito ◽  
A. Krishan
Keyword(s):  
Cell Lines ◽  
Primary Cultures ◽  
Virus Particles ◽  
Barr Virus ◽  
Virus Latency ◽  
Virus Surveillance ◽  
Cultured Human Cells ◽  
Epstein Barr ◽  
Serial Transplantation ◽  
Tubular Arrays

In conjunction with the cultivation and transplantation of cells from human tumors by the Programs of Microbiology and Immunogenetics, virus surveillance by electron microscopy has been routinely employed. Of particular interest in this regard have been 3 cell lines cultured from lymph nodes or spleen of 2 patients with Hodgkin's disease and 1 patient with Letterer-Siwe's disease. Each of these cell lines when transplanted in Syrian hamster neonates conditioned with anti-lymphocyte serum grew as serially transplantable tumors; from such transplants of the 3 cell lines cell cultures were retrieved.Herpes type virus particles (Figs. 1, 2, 3) were found in the primary cultures of all three lines, in frozen thawed aliquots of same, and in cultures retrieved from their tumors growing by serial transplantation in hamsters. No virus was detected in sections of 25 of the serially transplanted tumors. However, in 10 such tumors there were repeated instances of tubular arrays in the cisternae of the endoplasmic reticulum (Fig. 4). On serologic examination the herpes virus was shown to be the Epstein-Barr virus.

Comparison of Choriocarcinoma and Normal Placenta

1971 ◽  
Vol 29 ◽  
pp. 324-325
Author(s):  
John C. Garancis ◽  
Roland A. Pattillo ◽  
Robert O. Hussa ◽  
Jon V. Straumfjord
Keyword(s):  
Cell Lines ◽  
Small Cells ◽  
Tissue Cultures ◽  
Glycogen Depletion ◽  
Cell Surfaces ◽  
Intercellular Spaces ◽  
Concomitant Increase ◽  
Gestational Choriocarcinoma ◽  
Cytoplasmic Glycogen ◽  
Normal Placenta

Two different cell lines (Be-Wo and Jar) of human gestational choriocarcinoma have been maintained in continuous tissue culture for a period of four and two years respectively without losing the ability to elaborate human chorionic gonadotropin (HCG). Tissue cultures, as revealed by electron microscopy, consisted of small cells with single nuclei. In some instances cell surfaces were provided with microvilli but more often the intercellular spaces were narrow and bridged by desmosomes. However, syncytium was not formed. Endoplasmic reticulum (ER) was poorly developed in both cell lines, except in some Be-Wo cells it was prominent. Golgi complex, lysosomes and numerous free ribosomes, as well as excessive cytoplasmic glycogen, were present in all cells (Fig. 1). Glycogen depletion and concomitant increase of ER were observed in many cells following a single dose of 10 ugm/ml of adrenalin added to medium (Fig. 2).

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