An investigation of the growth characteristics of oil-palm (Elaeis guineensis) suspension cultures using 31P NMR

1983 ◽  
Vol 3 (12) ◽  
pp. 1141-1148 ◽  
Author(s):  
W. A. Hughes ◽  
S. M. Bociek ◽  
J. N. Barrett ◽  
R. G. Ratcliffe
Keyword(s):  
Suspension Culture ◽  
Oil Palm ◽  
Nmr Spectra ◽  
31P Nmr ◽  
Elaeis Guineensis ◽  
Growth Characteristics ◽  
Dichlorophenoxyacetic Acid ◽  
Meristematic Tissue ◽  
Seedling Roots ◽  
2,4 Dichlorophenoxyacetic Acid

High-resolution 31P nuclear-magnetic-resonance (NMR) spectra are reported for oil-palm (Elaeis guineensis) cells in suspension culture. The spectra are a signicant improvement on the results that have appeared for other cultures and they are comparable with the spectra of the meristematic tissue in seedling roots. The NMR technique was used in parallel with other analytical methods to investigate the growth characteristics of the suspension culture, indluding the effect of 2,4-dichlorophenoxyacetic acid.

INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ

2021 ◽  
Vol 45 ◽  
Author(s):  
Marlúcia Souza Pádua Vilela ◽  
Jéssica de Castro e Andrade ◽  
Raíssa Silveira Santos ◽  
Vanessa Cristina Stein ◽  
Patrick Callegari Magnani Santos Alves ◽  
...  
Keyword(s):  
Oil Palm ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Callus Formation ◽  
Leaf Explants ◽  
Dichlorophenoxyacetic Acid ◽  
In Vitro Cultivation ◽  
Low Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid

ABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and the storage of germplasm elites. This study aimed to induce embryogenic calluses from leaves of oil palm plants in low concentrations of auxins and to observe the maintenance of these characteristics during in vitro cultivation. Calluses were induced in 0.5 cm leaf explants in Y3 culture medium supplemented with Picloram (4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) or 2,4-D (2,4-dichlorophenoxyacetic acid), at concentrations of 0, 1, 3, 6, and 9 mg L-1. The callus with embryogenic appearance was subcultured and evaluated regarding maintenance of embryogenic characteristics by cytochemical analyses. The best treatment for induction of calluses was composed of 1mg.L-1 of Picloram, which led to 30% callus formation. The calluses were classified into4 types, based on color and morphology. The cells of calluses with nodular and beige appearance have embryogenic characteristics, and the embryogenic potential of the cell masses was maintained over the 20 months of cultivation. This differentiated adaptation to the protocol can allow the advance in the mass propagation of oil palm through tissue culture, indicating the importance of investigating the topics proposed by the research.

Kinetics of 2,4-Dichlorophenoxyacetic Acid Content in an Auxin-Dependent Suspension Culture of Nicotiana tabacum Cells

1995 ◽  
Vol 147 (3-4) ◽  
pp. 383-390 ◽  
Author(s):  
K.B. Koens ◽  
F.T. Nicoloso ◽  
Th. B. Van Vliet ◽  
M. Harteveld ◽  
C.J.M. Boot ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Suspension Culture ◽  
Acid Content ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Kinetics Of

scholarly journals PROLIFERATION OF OIL PALM (Elaeis guineensis Jacq.) EMBRYOGENIC CALLUS WITH REPEATED SUBCULTURES IN LIQUID MEDIUM

2021 ◽  
Vol 8 (1) ◽  
pp. 1-11
Author(s):  
Karyanti Karyanti ◽  
Teuku Tajuddin ◽  
Hayat Khairiyah ◽  
Tati Sukarnih ◽  
Gemilang Rahmadara ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Oil Palm ◽  
Embryogenic Callus ◽  
Elaeis Guineensis ◽  
High Quality ◽  
Palm Trees ◽  
Callus Production ◽  
Callus Proliferation ◽  
Ability To Regenerate ◽  
Proliferation Ability

The availability of high-quality seeds is now a necessity. This is due to a government program to replace oil palm trees in smallholder plantations with high quality seeds. An efficient protocol to produce a large number of embryos is needed. To increase the number of embryogenic callus production, the callus proliferation experiment was carried out through suspension culture. This study aimed to examine the proliferation ability of embryogenic callus from three different oil palm clones, in several repeated subcultures. Liquid MS media added with 1 ppm 2.4-D and 0.1 ppm NAA were used. Embryogenic callus was weighed by 0.1 - 0.2 g, transferred into the liquid media, shaking at 60-80 rpm and 27 ºC for 8 weeks without light. Continues subcultures were repeated up to 7 times. The results showed that the growth rate of embryogenic callus increased in the third and fourth subcultures and then decreased in subsequent subcultures. It also revealed that the entire embryogenic callus from the first subculture up to seventh subculture still has the ability to regenerate into new plants. These results indicate that oil palm embryogenic callus can be proliferated by suspension culture with a limit up to the fourth subculture. Ketersediaan benih kelapa sawit berkualitas saat ini merupakan kebutuhan karena adanya program pemerintah untuk menggantikan tanaman sawit di kebun-kebun petani. Salah satu cara vegetatif yang dapat dilakukan adalah meningkatkan jumlah kalus embriogenik yang dihasilkan melalui pengembangan kultur suspensi. Penelitian ini bertujuan mengkaji kemampuan proliferasi kalus embriogenik dari tiga klon kelapa sawit, pada beberapa kali subkultur yang berulang. Media cair MS dengan penambahan 1 ppm 2,4-D dan 0,1 ppm NAA digunakan untuk memperbanyak 0,1–0,2 g kalus embriogenik, dikocok pada 60-80 rpm dan suhu 27 ºC tanpa cahaya selama 8 minggu. Subkultur berulang dilakukan hingga 7 kali. Hasil percobaan menunjukkan bahwa kemampuan proliferasi kalus dipengaruhi oleh genotip tanaman induk. Rata-rata kalus embriogenik dapat meningkat pada subkultur ke-3 dan ke-4 dan semakin menurun pada subkultur selanjutnya. Kalus embriogenik hasil proliferasi subkultur pertama hingga ke-7 dapat tumbuh menjadi calon tanaman baru. Hasil ini menunjukkan bahwa kalus embriogenik kelapa sawit dapat diperbanyak dengan kultur suspensi pada batas sampai subkultur ke-4.

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