Effect of temperature on the incorporation into phospholipid classes and metabolismvia desaturation and elongation of n−3 and n−6 polyunsaturated fatty acids in fish cells in culture

Lipids ◽  
1990 ◽  
Vol 25 (8) ◽  
pp. 435-442 ◽  
Author(s):  
Douglas R. Tocher ◽  
John R. Sargent
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Effect Of Temperature ◽  
Cells In Culture ◽  
Phospholipid Classes ◽  
Fish Cells

Effect of Polyunsaturated Fatty Acids on Fetal Mouse Brain Cells in Culture in a Chemically Defined Medium

1983 ◽  
Vol 41 (5) ◽  
pp. 1234-1242 ◽  
Author(s):  
J. M. Bourre ◽  
A. Faivre ◽  
O. Dumont ◽  
A. Nouvelot ◽  
C. Loudes ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Mouse Brain ◽  
Defined Medium ◽  
Brain Cells ◽  
Chemically Defined Medium ◽  
Fetal Mouse ◽  
Cells In Culture

Effects of dietary n−3 and n−6 polyunsaturated fatty acids on macrophage phospholipid classes and subclasses

Lipids ◽  
1990 ◽  
Vol 25 (12) ◽  
pp. 827-834 ◽  
Author(s):  
Robert S. Chapkin ◽  
Suzan L. Carmichael
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Phospholipid Classes

Comparative study of the lipid composition of rabbit and lobster sarcoplasmic reticulum

1976 ◽  
Vol 54 (5) ◽  
pp. 516-520 ◽  
Author(s):  
Vitor M. C. Madeira ◽  
M. C. Antunes-Madeira
Keyword(s):  
Fatty Acids ◽  
Sarcoplasmic Reticulum ◽  
Polyunsaturated Fatty Acids ◽  
Comparative Study ◽  
Molecular Species ◽  
Lipid Composition ◽  
Unsaturated Fatty Acids ◽  
Phospholipid Classes ◽  
Plasmalogen Content

Sarcoplasmic reticulum (SR) membranes isolated from rabbit and lobster muscles have similar phospholipid classes, but they differ in plasmalogen content. The plasmalogenic species are mostly distributed among phosphatidylethanolamines (PE's) and make up about 62% of the total in rabbit SR and about 46% in lobster membranes. Lobster SR phospholipids contain large amounts of polyunsaturated fatty acids which are present in low amounts in rabbit membranes. The total unsaturated fatty acids of phosphatidylcholines (PC's) represent about 53% and 73% of the total fatty chains for rabbit and lobster SR, respectively. The values found for PE's were about 56% and 64%., respectively. Furthermore, lobster membranes contain significant amounts of PC and PE molecular species with unsaturated fatty acids in positions 1 and 2, whereas rabbit SR contain low amounts.

scholarly journals Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds

2005 ◽  
Vol 17 (2) ◽  
pp. 213-222 ◽  
Author(s):  
Anna Cristina Lanna ◽  
Inês Chamel José ◽  
Maria Goreti de Almeida Oliveira ◽  
Everaldo Gonçalves Barros ◽  
Maurilio Alves Moreira
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Low Temperature ◽  
Polyunsaturated Fatty Acids ◽  
Linolenic Acid ◽  
Acid Content ◽  
Genetic Manipulation ◽  
Effect Of Temperature ◽  
Linolenic Acid Content ◽  
Development Stages

Soybean oil contains around 60 % of polyunsaturated fatty acids, which are responsible for the low oxidative stability of soy-derived products. Soybean lines with low linolenic acid content can be obtained by genetic manipulation; however, a high proportion of the variation in fatty acids content is due to environmental factors. This work aimed to determine the effect of temperature on oil composition and on the activity of the enzymes CDP-choline:1,2-diacylglycerolcholine phosphotransferase (CPT) and acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), responsible for maintenance of polyunsaturated fatty acids in the cytoplasmic acyl-CoA pool, that is used for oil synthesis in the seeds. CAC-1, a soybean variety with linolenic acid content of about 8 % and CC4, a BC3F4 CAC-1 derived line, with about 4 % linolenic acid, were used. The lines were cultivated under two temperature: 34/28ºC or 22/13ºC - day/night. The seeds were collected along seven development stages, according to their fresh weight. Fatty acid analysis was carried out by gas chromatography and CPT and LPCAT activities were determined by measuring the radioactivity incorporated in their products, phosphatidyl-[14C]choline and phosphatidylcholine-[14C]oleoyl, respectively. Linolenic acid contents were 3.89 and 6.92 % for line CC4 and 7.39 and 12.49 % for variety CAC-1, when submitted to high and low temperature conditions, respectively. Both enzymes were more active, in the development stages analyzed, in seeds produced under low temperature. Kinetics characterization of CPT and LPCAT were conducted previously.

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