A serum-free primary culture system for studying cell-substrate interactions during newt epidermal cell migration

1988 ◽  
Vol 24 (10) ◽  
pp. 1023-1030 ◽  
Author(s):  
James T. Mahan ◽  
Donald J. Donaldson
Keyword(s):  
Cell Migration ◽  
Primary Culture ◽  
Epidermal Cell ◽  
Culture System ◽  
Substrate Interactions ◽  
Serum Free ◽  
Cell Substrate

Role of carbohydrates in cell-substrate interactions during newt epidermal cell migration

1987 ◽  
Vol 243 (3) ◽  
pp. 461-471 ◽  
Author(s):  
Karen Davenport Atnip ◽  
James T. Mahan ◽  
Donald J. Donaldson
Keyword(s):  
Cell Migration ◽  
Epidermal Cell ◽  
Substrate Interactions ◽  
Cell Substrate

Effects of Friction Coefficient and Receptor Number on Cell-Substrate Interactions During Migration

2010 ◽  
Author(s):  
Henry C. Wong ◽  
William C. Tang
Keyword(s):  
Cell Migration ◽  
Cancer Metastasis ◽  
Element Model ◽  
Biological Tissues ◽  
Substrate Interactions ◽  
Strain Behavior ◽  
Structural Support ◽  
Research Areas ◽  
Cell Substrate ◽  
A Cell

Biological tissues are composed of cells that adhere to the extracellular matrix (ECM) via cell-surface integrin receptors that bind to specific proteins, such as fibronectin, embedded in the matrix. In this manner, the ECM functions as a structural support for the attached cells, and mechanical forces are able to be transmitted from the cell to the ECM and vice versa [1]. Cell migration, a process that is highly dependent on these mechanical interactions, is important for many normal biological processes and diseases that occur in the human body, which include embryonic development, immune response, would healing, and cancer invasion [2]. Though many continuum models of cell migration have been proposed, there is still a need for a model that can be used to quantitatively understand the mechanical factors that can influence the movement of a cell on a substrate. This would be invaluable to the research areas of tissue engineering as well as cancer metastasis. We utilized a finite element model to elucidate the mechanism of cell-substrate interactions for a cell that consistently migrates in a single direction. Our model follows the approach taken by Gracheva and Othmer [2], but we extended their model to describe two-dimensional plane strain behavior.

scholarly journals Spheroids of Endothelial Cells and Vascular Smooth Muscle Cells Promote Cell Migration in Hyaluronic Acid and Fibrinogen Composite Hydrogels

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Xingang Zuo ◽  
Haolan Zhang ◽  
Tong Zhou ◽  
Yiyuan Duan ◽  
Hao Shou ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Migration ◽  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Dynamic Change ◽  
Substrate Interactions ◽  
Composite Hydrogels ◽  
Cell Functions ◽  
Cell Spheroids ◽  
Cell Substrate

Cell migration plays a pivotal role in many pathological and physiological processes. So far, most of the studies have been focused on 2-dimensional cell adhesion and migration. Herein, the migration behaviors of cell spheroids in 3D hydrogels obtained by polymerization of methacrylated hyaluronic acid (HA-MA) and fibrinogen (Fg) with different ratios were studied. The Fg could be released to the medium gradually along with time prolongation, achieving the dynamic change of hydrogel structures and properties. Three types of cell spheroids, i.e., endothelial cell (EC), smooth muscle cell (SMC), and EC-SMC spheroids, were prepared with 10,000 cells in each, whose diameters were about 343, 108, and 224 μm, respectively. The composite hydrogels with an intermediate ratio of Fg allowed the fastest 3D migration of cell spheroids. The ECs-SMCs migrated longest up to 3200 μm at day 14, whereas the SMC spheroids migrated slowest with a distance of only ~400 μm at the same period of time. The addition of free RGD or anti-CD44 could significantly reduce the migration distance, revealing that the cell-substrate interactions take the major roles and the migration is mesenchymal dependent. Moreover, addition of anti-N-cadherin and MMP inhibitors also slowed down the migration rate, demonstrating that the degradation of hydrogels and cell-cell interactions are also largely involved in the cell migration. RT-PCR measurement showed that expression of genes related to cell adhesion and antiapoptosis, and angiogenesis was all upregulated in the EC-SMC spheroids than single EC or SMC spheroids, suggesting that the use of composite cell spheroids is more promising to promote cell-substrate interactions and maintenance of cell functions.

scholarly journals Cell-Substrate Interactions Feedback to Direct Cell Migration along or against Morphological Polarization

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0133117 ◽  
Author(s):  
Girish Kumar ◽  
Chia-Chi Ho ◽  
Carlos C. Co
Keyword(s):  
Cell Migration ◽  
Substrate Interactions ◽  
Cell Substrate ◽  
Direct Cell

Cell types and cell-substrate interactions in serum-free dissociated cultures of rat hypothalamus

1987 ◽  
Vol 436 (2) ◽  
pp. 339-351 ◽  
Author(s):  
Roseann Ventimiglia ◽  
Herbert M. Geller
Keyword(s):  
Cell Types ◽  
Substrate Interactions ◽  
Serum Free ◽  
Rat Hypothalamus ◽  
Cell Substrate

Primary culture of rat ependymal cells in serum-free defined medium

1986 ◽  
Vol 390 (2) ◽  
pp. 199-209 ◽  
Author(s):  
M WEIBEL ◽  
B PETTMANN ◽  
J ARTAULT ◽  
M SENSENBRENNER ◽  
G LABOURDETTE
Keyword(s):  
Primary Culture ◽  
Defined Medium ◽  
Ependymal Cells ◽  
Serum Free

Production and Analysis of High Resolution Polymer Replicas of Fibrillar Collagen

1999 ◽  
Vol 5 (S2) ◽  
pp. 398-399
Author(s):  
P. Sims ◽  
B. Todd ◽  
S. Eppell ◽  
T. Li ◽  
K. Park ◽  
...  
Keyword(s):  
Cellular Response ◽  
Physical Nature ◽  
Cell Types ◽  
Artificial Substrates ◽  
Adherent Cell ◽  
Fibrillar Collagen ◽  
New Materials ◽  
Substrate Interactions ◽  
Number Of Factors ◽  
Cell Substrate

Adherent cells generally construct the immediate substrate upon which they reside. This may occur via synthesis and secretion of new materials and/or by rearrangement and modification of existing substrate. The response of adherent cell types to an existing substrate can be influenced by a number of factors which include both the chemical and physical nature of the substrate. Cell adhesion, proliferation, differentiation and death can all be substrate dependent. Much effort has been directed toward chemical modification of substrates to regulate one or more of the parameters noted above. A significant, but somewhat smaller, degree of attention has been paid to the effects of the topography and microtopography on the cell response to substrate materials. Studies to date strongly suggest the topography is a significant factor in cell-substrate interactions. As noted above, it is most probable that both the chemistry and the structure of a substrate simultaneously influence the cellular response. However we wished to determine, particularly for artificial substrates, the role which microtopography can play in cell-substrate interactions.

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