Further purification of a fibroblast growth factor-like factor from chick embryo extract by heparin-affinity chromatography

1989 ◽  
Vol 25 (3) ◽  
pp. 236-242 ◽  
Author(s):  
Ichiro Kimura ◽  
Yae Gotoh ◽  
Eijiro Ozawa
Keyword(s):  
Growth Factor ◽  
Chick Embryo ◽  
Affinity Chromatography ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Embryo Extract ◽  
Chick Embryo Extract ◽  
Heparin Affinity

scholarly journals Isolation of brain fibroblast growth factor by heparin-Sepharose affinity chromatography: identity with pituitary fibroblast growth factor.

1984 ◽  
Vol 81 (22) ◽  
pp. 6963-6967 ◽  
Author(s):  
D. Gospodarowicz ◽  
J. Cheng ◽  
G. M. Lui ◽  
A. Baird ◽  
P. Bohlent
Keyword(s):  
Growth Factor ◽  
Affinity Chromatography ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth

Hypoblastic tissue and fibroblast growth factor induce blood tissue (haemoglobin) in the early chick embryo

Development ◽  
1994 ◽  
Vol 120 (12) ◽  
pp. 3571-3579 ◽  
Author(s):  
C. Gordon-Thomson ◽  
B.C. Fabian
Keyword(s):  
Growth Factor ◽  
Chick Embryo ◽  
Fibroblast Growth Factor ◽  
Culture Medium ◽  
Marginal Zone ◽  
Inner Core ◽  
Fibroblast Growth ◽  
High Propensity ◽  
Series Of Experiments

We have investigated the temporal and the causal basis of blood tissue specification in the chick embryo. Earlier workers have shown that the prospective blood-forming area is specified in a horseshoe-shaped area at the posterior side of the embryo. We found that cultured explants from the posterior marginal zone at stages XI to XIII (consisting of the posterior marginal zone and part of Koller's sickle) have a high propensity to form haemoglobin (Hb), which could be inhibited at stage XI by adding antibody against basic fibroblast growth factor (bFGF) to the neutral culture medium; this treatment had no effect from stage XII onwards. The same result was found when whole embryos were cultured with an antiserum raised against bFGF, or with heparin. In another series of experiments, we found that cultured pieces from the inner-core of stage XIII epiblasts (with or without hypoblast tissue) were able to form Hb, whereas inner-core pieces from the pre-hypoblast stages, namely stages X and XI, did not form Hb. The capacity to form Hb, however, could be conferred upon the inner-core pieces from stage X epiblasts if bFGF at a concentration of 75–150 ng/ml was added to the culture medium. Furthermore, and most pertinently, the capacity to form Hb could be conferred on stage X inner-core pieces when they were co-cultured with hypoblast from a stage XIII embryo in a sandwich explant. Thus the inductive role of the hypoblast appears to be mediated via bFGF.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Role of fibroblast growth factor signaling during proepicardium formation in the chick embryo

2010 ◽  
Vol 239 (9) ◽  
pp. 2393-2403 ◽  
Author(s):  
Angela Torlopp ◽  
Jan Schlueter ◽  
Thomas Brand
Keyword(s):  
Growth Factor ◽  
Chick Embryo ◽  
Fibroblast Growth Factor ◽  
Growth Factor Signaling ◽  
Fibroblast Growth ◽  
Fibroblast Growth Factor Signaling
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