Analysis, characterization and diagnostic use of circulating filarial antigen in bancroftian filariasis

1990 ◽  
Vol 15 (1) ◽  
pp. 37-46 ◽  
Author(s):  
K. A. Parkhe ◽  
M. V. R. Reddy ◽  
K. Cheirmaraj ◽  
P. Ramaprasad ◽  
B. C. Harinath
Keyword(s):  
Bancroftian Filariasis ◽  
Diagnostic Use ◽  
Filarial Antigen ◽  
Circulating Filarial Antigen

scholarly journals Incidence of filariasis in clinically diagnosed primary vaginal hydrocele

2020 ◽  
Vol 7 (3) ◽  
pp. 640
Author(s):  
Mahendra Bendre ◽  
Shrreya Akhil ◽  
Srujan Kondreddy
Keyword(s):  
Histopathological Examination ◽  
Peripheral Blood Smear ◽  
Cross Sectional Study ◽  
Research Centre ◽  
Bancroftian Filariasis ◽  
College Hospital ◽  
Cross Sectional ◽  
Medical College ◽  
Filarial Antigen ◽  
Circulating Filarial Antigen

Background: Lymphatic filariasis is caused by a mosquito-borne parasite affecting roughly 100 million people round the world. There is consensus that hydrocele is the most frequent clinical manifestation of bancroftian filariasis. In endemic areas, about 40% of men are suffering from testicular hydrocele. With this background, the present study was aimed to find the incidence of filariasis in clinically diagnosed primary vaginal hydrocele.Methods: A hospital based prospective, cross-sectional study was conducted with 60 patients diagnosed clinically as primary vaginal hydrocele coming to the department of surgery, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pimpri, Pune, to assess the incidence of filariasis.Results: Anti-filarial antibody and circulating filarial antigen in serum were detected in 5 (8.3%). Out of 60 patients and anti-filarial antibody was detected in hydrocele fluid of 2 (3.3%) patients. 2 patients out of these 5 showed microfilaria in peripheral blood smear and eosinophilic infiltrates in histopathological examination of sac.Conclusions: In 5 out of 60 cases both anti-filarial antibody and circulating filarial antigen in serum are positive thus proving that incidence of filarial hydrocele is 8% in clinically diagnosed primary vaginal hydrocele which is supposed to be idiopathic. Even though these cases have presented as clinically primary vaginal hydrocele, they are found to be filarial hydrocele after analysis of serum and hydrocele fluid. So, it is advised that all cases of clinically diagnosed primary vaginal hydroceles should be investigated for filariasis and if not, may lead to recurrence in these cases.

scholarly journals Circulating filarial antigen in the hydrocele fluid from individuals living in a bancroftian filariasis area - Recife, Brazil: detected by the monoclonal antibody Og4C3-assay

2004 ◽  
Vol 99 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Abraham Rocha ◽  
Guilherme Lima ◽  
Zulma Medeiros ◽  
Ana Aguiar-Santos ◽  
Sandra Alves ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Bancroftian Filariasis ◽  
Filarial Antigen ◽  
Circulating Filarial Antigen

Circulating filarial antigen detection in brugian filariasis

Parasitology ◽  
2015 ◽  
Vol 143 (3) ◽  
pp. 350-357
Author(s):  
PRAVEEN KUMAR TRIPATHI ◽  
RAMESH CHANDER MAHAJAN ◽  
NANCY MALLA ◽  
ABHISHEK MEWARA ◽  
SHAILJA MISRA BHATTACHARYA ◽  
...  
Keyword(s):  
Adult Worm ◽  
Specific Antigen ◽  
Antigen Detection ◽  
Serum Samples ◽  
Helminthic Infections ◽  
Filarial Antigen ◽  
Endemic Normal ◽  
Grade Iii ◽  
Antigen Reactivity ◽  
Circulating Filarial Antigen

SUMMARYHuman lymphatic filariasis (LF) is a major cause of disability globally. The success of global elimination programmes for LF depends upon effectiveness of tools for diagnosis and treatment. In this study on stage-specific antigen detection in brugian filariasis, L3, adult worm (AW) and microfilarial antigenaemia were detected in around 90–95% of microfilariae carriers (MF group), 50–70% of adenolymphangitis (ADL) patients, 10–25% of chronic pathology (CP) patients and 10–15% of endemic normal (EN) controls. The sensitivity of the circulating filarial antigen (CFA) detection in serum samples from MF group was up to 95%. In sera from ADL patients, unexpectedly, less antigen reactivity was observed. In CP group all the CFA positive individuals were from CP grade I and II only and none from grade III or IV, suggesting that with chronicity the AWs lose fecundity and start to disintegrate and die. Amongst EN subject, 10–15% had CFA indicating that few of them harbour filarial AWs, thus they might not be truly immune as has been conventionally believed. The specificity for antigen detection was 100% when tested with sera from various other protozoan and non-filarial helminthic infections.

scholarly journals Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae

2009 ◽  
Vol 104 (4) ◽  
pp. 621-625 ◽  
Author(s):  
Abraham Rocha ◽  
Cynthia Braga ◽  
Marcela Belém ◽  
Arturo Carrera ◽  
Ana Aguiar-Santos ◽  
...  
Keyword(s):  
Lymphatic Filariasis ◽  
Filarial Antigen ◽  
Comparison Of Tests ◽  
Circulating Filarial Antigen

scholarly journals Semi-Quantitative Scoring of an Immunochromatographic Test for Circulating Filarial Antigen

2013 ◽  
Vol 89 (5) ◽  
pp. 916-918 ◽  
Author(s):  
Cédric B. Chesnais ◽  
Jean Bopda ◽  
Andrew C. Majewski ◽  
Sébastien D. S. Pion ◽  
Michel Boussinesq ◽  
...  
Keyword(s):  
Immunochromatographic Test ◽  
Filarial Antigen ◽  
Quantitative Scoring ◽  
Circulating Filarial Antigen

scholarly journals Determination of the prevalence of lymphatic filariasis among migrant workers in Kuwait by detecting circulating filarial antigen

2006 ◽  
Vol 55 (4) ◽  
pp. 401-405 ◽  
Author(s):  
Jamshaid Iqbal ◽  
Ali Sher
Keyword(s):  
Sensitivity And Specificity ◽  
Migrant Workers ◽  
Indian Subcontinent ◽  
Wuchereria Bancrofti ◽  
Parasitic Infections ◽  
Filarial Infection ◽  
Filarial Antigen ◽  
Sensitivity Specificity ◽  
Circulating Filarial Antigen

The main objective of this study was to determine the prevalence of filarial infection among migrant workers in Kuwait. The study was conducted from April 2000 to November 2003. A total of 1050 migrant workers (>90 % from the Indian subcontinent) from filarial endemic countries and 260 individuals residing in Kuwait as controls (50 healthy Kuwaiti blood donors, 50 microfilaria-negative individuals from endemic areas and 160 patients with other parasitic infections) were screened for filarial infection. All specimens were tested for microfilaraemia by microscopy of nucleopore-filtered blood (NFB) and detection of circulating filarial antigen (CFA) by an immunochromatographic test (ICT) and the TropBio assay. The overall prevalence of filarial antigenaemia was 18·3 % (192 individuals) using the ICT and 20·3 % (213 individuals) using the TropBio assay. Thirty-two cases (3 %) of Wuchereria bancrofti were detected by microscopy and the mean microfilaria count in these cases was 816 microfilariae ml−1. CFA was detected only in two of the 260 control subjects. Statistical analysis to calculate the sensitivity, specificity and prevalence of infection was carried out using maximum-likelihood statistical methods. The overall sensitivity and specificity of the ICT and TropBio assay to detect CFA were comparable. Compared with NFB microscopy, the sensitivity of the ICT was 93·8 % and specificity ranged from 84 to 100 %. The sensitivity and specificity of the TropBio assay were 90·1 and 100 %, respectively. However, the ICT failed to detect CFA in two cases with a microfilarial load of <20 microfilariae ml−1. In conclusion, the prevalence of filarial infection among the migrant workers in Kuwait was 18·3 % as determined by the ICT.

Detection of circulating antigen in bancroftian filariasis by sandwich ELISA using filarial serum IgG

1984 ◽  
Vol 58 (3) ◽  
pp. 259-262 ◽  
Author(s):  
M. V. R. Reddy ◽  
Ashok Malhotra ◽  
B. C. Harinath
Keyword(s):  
Immunosorbent Assay ◽  
Sandwich Elisa ◽  
Negative Reaction ◽  
Serum Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bancroftian Filariasis ◽  
Positive Correlation ◽  
Serum Igg ◽  
Filarial Antigen ◽  
Circulating Antigen

ABSTRACTThe utility of the IgG fraction of human filarial serum immunoglobulin in detecting circulating antigen by sandwich enzyme linked immunosorbent assay (ELISA) was studied. 27 of 33 sera from persons with microfilaracmia, 19 of 30 sera from clinical cases of filariasis, 4 of 30 sera from normal persons from a region endemic for filariasis showed the presence of circulating filarial antigen. All the 20 normal sera from the area where filariasis was not endemic gave negative reaction for filarial antigen. Those sera from persons with microfilaracmia that showed the presence of circulating antigen also showed an apparent positive correlation between the microfilarial density and the antigen titre.

A 43-kDa circulating filarial antigen fraction of Wuchereria bancrofti in immunoprophylaxis against Brugia malayi in jirds

2000 ◽  
Vol 48 (4) ◽  
pp. 281-288 ◽  
Author(s):  
Chenthamarakshan Vasu ◽  
Maryata Venkatarami Reddy ◽  
Baskar Chinnaiah Harinath
Keyword(s):  
Brugia Malayi ◽  
Wuchereria Bancrofti ◽  
Filarial Antigen ◽  
Circulating Filarial Antigen
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