Development of a test strip reader for a lateral flow membrane-based immunochromatographic assay

2004 ◽  
Vol 9 (2) ◽  
pp. 127-131 ◽  
Author(s):  
Suhyeon Kim ◽  
Je-Kyun Park
Keyword(s):  
Test Strip ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Test Strip Reader

Development and study of lateral flow test strip reader based on embedded system

2011 ◽  
Author(s):  
Mei Jianchun ◽  
Ye Qing ◽  
Zhou Wenyuan ◽  
Hui Wangwei ◽  
Tian Jianguo
Keyword(s):  
Embedded System ◽  
Test Strip ◽  
Lateral Flow ◽  
Flow Test ◽  
Test Strip Reader ◽  
Lateral Flow Test

scholarly journals Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Kyung Mi Park ◽  
Da Jung Chung ◽  
Mijin Choi ◽  
Taejoon Kang ◽  
Jinyoung Jeong
Keyword(s):  
Chemical Species ◽  
Test Strip ◽  
Disease Diagnosis ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Diagnosis And Prognosis ◽  
Lateral Flow Immunochromatographic Assay

Abstract A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1–10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.

scholarly journals Diagnostic accuracy of an app-guided, self-administered test for influenza among individuals presenting to general practice with influenza-like illness: study protocol

BMJ Open ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. e036298
Author(s):  
Victoria Lyon ◽  
Monica Zigman Suchsland ◽  
Monique Chilver ◽  
Nigel Stocks ◽  
Barry Lutz ◽  
...  
Keyword(s):  
Influenza A ◽  
Test Strip ◽  
Lateral Flow ◽  
Research Network ◽  
Lateral Flow Immunoassay ◽  
Rt Pcr ◽  
Test Procedures ◽  
Influenza Like Illness ◽  
Test Kit ◽  
Test Strip Reader

IntroductionDiagnostic tests for influenza in Australia are currently only authorised for use in clinical settings. At-home diagnostic testing for influenza could reduce the need for patient contact with healthcare services, which potentially could contribute to symptomatic improvement and reduced spread of influenza. We aim to determine the accuracy of an app-guided nasal self-swab combined with a lateral flow immunoassay for influenza conducted by individuals with influenza-like illness (ILI).Methods and analysisAdults (≥18 years) presenting with ILI will be recruited by general practitioners (GP) participating in Australian Sentinel Practices Research Network. Eligible participants will have a nasal swab obtained by their GP for verification of influenza A/B status using reverse transcription polymerase chain reaction (RT-PCR) test at an accredited laboratory. Participants will receive an influenza test kit and will download an app that collects self-reported symptoms and influenza risk factors, then instructs them in obtaining a low-nasal self-swab, running a QuickVue influenza A+B lateral flow immunoassay (Quidel Corporation) and interpreting the results. Participants will also interpret an enhanced image of the test strip in the app. The primary outcome will be the accuracy of participants’ test interpretation compared with the laboratory RT-PCR reference standard. Secondary analyses will include accuracy of the enhanced test strip image, accuracy of an automatic test strip reader algorithm and validation of prediction rules for influenza based on self-reported symptoms. A post-test survey will be used to obtain participant feedback on self-test procedures.Ethics and disseminationThe study was approved by the Human Research and Ethic Committee (HREC) at the University of Adelaide (H-2019-116). Protocol details and any amendments will be reported to https://www.tga.gov.au/. Results will be published in the peer-reviewed literature, and shared with stakeholders in the primary care and diagnostics communities.Trial registration numberAustralia New Zealand Clinical Trial Registry (U1111-1237-0688).

scholarly journals Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Foods ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
Jiali Li ◽  
Biao Ma ◽  
Jiehong Fang ◽  
Antong Zhi ◽  
Erjing Chen ◽  
...  
Keyword(s):  
Test Line ◽  
Point Of Care ◽  
Limited Resource ◽  
Test Strip ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Control Line ◽  
Test Strip Reader

Salmonella can cause serious foodborne diseases. We have developed a lateral flow immunoassay combined with recombinase polymerase amplification (LFD-RPA) for detection of Salmonella in food. The conserved fragment (fimY) was selected as the target gene. Under an optimal condition (37 °C, 10 min), the sensitivity was 12 colony-forming units (CFU)/mL in a pure culture. Testing with 16 non-Salmonella strains as controls revealed that LFD-RPA was specific to the fimY gene of Salmonella. The established assay could detect Salmonella at concentrations as low as 1.29 × 102 CFU/mL in artificially contaminated samples. This detection was at a slightly higher level than that for a pure bacterial culture. Combined with the test strip reader, the LFD-RPA is a feasible method for quantitative detection of Salmonella based on the test line intensity, which was the ratio for the test line and control line of the reflected light. The method could be a potential point-of-care test in limited resource areas and provides a new approach and technical support for the diagnosis of food safety.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Effect of physiochemical property of Fe3O4 particle on magnetic lateral flow immunochromatographic assay

2014 ◽  
Vol 197 ◽  
pp. 129-136 ◽  
Author(s):  
Jun Yan ◽  
Yingying Liu ◽  
Yilong Wang ◽  
Xiaowei Xu ◽  
Ying Lu ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Fe3o4 Particle ◽  
Physiochemical Property ◽  
Lateral Flow Immunochromatographic Assay

Rapid and visual detection of benzothiostrobin residue in strawberry using quantum dot-based lateral flow test strip

2019 ◽  
Vol 283 ◽  
pp. 222-229 ◽  
Author(s):  
JiaKai Wu ◽  
JingWei Ma ◽  
Hong Wang ◽  
DongMei Qin ◽  
Li An ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Visual Detection ◽  
Test Strip ◽  
Lateral Flow ◽  
Flow Test ◽  
Lateral Flow Test

scholarly journals Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the detection of citrinin in cereals

2017 ◽  
Vol 28 (5) ◽  
pp. 754-766 ◽  
Author(s):  
Dezhao Kong ◽  
Zhengjun Xie ◽  
Liqiang Liu ◽  
Shanshan Song ◽  
Hua Kuang
Keyword(s):  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Lateral Flow Immunochromatographic Assay

Automated Water Test Strip Reader

2021 ◽  
Author(s):  
Rashi Singhal ◽  
Jay Doshi ◽  
Ishanali Kamani ◽  
Ninad Mehendale
Keyword(s):  
Test Strip ◽  
Test Strip Reader ◽  
Water Test
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