In vitro effect of pH and ethanol on biofilm formation by clinicalica-positiveStaphylococcus epidermidis strains

2007 ◽  
Vol 57 (3) ◽  
pp. 431-437 ◽  
Author(s):  
Kamel Chaieb ◽  
Olfa Chehab ◽  
Tarek Zmantar ◽  
Mahmoud Rouabhia ◽  
Kacem Mahdouani ◽  
...  
2017 ◽  
Vol 30 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Maja Bogdan ◽  
Domagoj Drenjancevic ◽  
Ivana Harsanji Drenjancevic ◽  
Branka Bedenic ◽  
Vlasta Zujic Atalic ◽  
...  

2015 ◽  
Vol 8 (2) ◽  
pp. 182-187
Author(s):  
V. V. Kuz’mina ◽  
G. V. Zolotareva ◽  
V. A. Sheptitsky

2015 ◽  
Vol 60 (3) ◽  
pp. 471-478 ◽  
Author(s):  
Ermelinda Matsuura ◽  
Janine Silva Ribeiro Godoy ◽  
Patrícia de Souza Bonfim-Mendonça ◽  
João Carlos Palazzo de Mello ◽  
Terezinha Inez Estivalet Svidzinski ◽  
...  

2014 ◽  
Vol 63 (1) ◽  
pp. 21-25 ◽  
Author(s):  
ANNA KUREK ◽  
KATARZYNA MARKOWSKA ◽  
ANNA M. GRUDNIAK ◽  
WIRGINIA JANISZOWSKA ◽  
KRYSTYNA I. WOLSKA

Oleanolic acid and ursolic acid are pentacyclic triterpenoids isolated from a variety of medicinal plants, which have antibacterial activity. Listeria monocytogenes is a Gram-positive facultative pathogen, being the causative agent of listeriosis. The present study was carried out to evaluate the in vitro effect of sub-inhibitory concentrations of both triterpene acids on the pathogenicity determinants of L. monocytogenes: their hemolytic activity and biofilm forming ability. Oleanolic and ursolic acids inhibited listeriolysin O activity without influencing toxin secretion. Biofilm formation, and the viability of L. monocytogenes cells in biofilms was diminished by both compounds. Thus, both acids affected L. monocytogenes virulence. It was also demonstrated that oleanolic acid bound to the peptidoglycan of L. monocytogenes and this interaction was influenced by teichoic acids.


Author(s):  
Guruge Niluka Dilrukshi ◽  
Jananie Kottahachchi ◽  
Thushari Dissanayake ◽  
Manjula Weerasekera ◽  
Mudara Peiris ◽  
...  

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 582
Author(s):  
Karlis Racenis ◽  
Juta Kroica ◽  
Dace Rezevska ◽  
Lauris Avotins ◽  
Edgars Skuditis ◽  
...  

Peritonitis caused by Staphylococcusaureus is of major importance in peritoneal dialysis (PD) patients due to its great virulence profile and biofilm formation ability. Bacteriophages are a potential tool to treat peritonitis resulting from biofilm-associated infections. We screened S. aureus colonization in 71 PD patients from the nasal cavity, groin, and PD exit-site regions and analyzed clinical outcomes in these patients. We performed biofilm-formation testing of different strains and compared the isolates of one patient to detect phenotypic differences in S. aureus. Phage cocktails were used to detect S. aureus in vitro susceptibility. An adaptation procedure was performed in cases of bacterial resistance. Around 30% of PD patients (n = 21) were found to be S. aureus carriers; from these, a total of 34 S. aureus strains were isolated, of which 61.8% (n = 21) produced a strong biofilm. Phenotypic differences in strain biofilm production were detected in eight patients out of ten. All strains were sensitive to commonly used antibiotics. Broadly positive phage lytic activity (100%) was observed in six cocktails out of seven, and bacterial resistance towards phages was overcome using adaptation. Overall phages showed a promising in vitro effect in biofilm-forming S. aureus strains.


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