A method of direct-viewing comparative analysis of tumour cell motility in vitro; effect of pH and adhesion on cells of different malignancy

1998 ◽  
Vol 3 (8) ◽  
pp. 1-13
Author(s):  
L. Pavlíková ◽  
D. Zicha ◽  
A. Chaloupková ◽  
P. Vesely
Keyword(s):  
Comparative Analysis ◽  
Cell Motility ◽  
Tumour Cell ◽  
Effect Of Ph ◽  
Vitro Effect

In vitro effect of pH and ethanol on biofilm formation by clinicalica-positiveStaphylococcus epidermidis strains

2007 ◽  
Vol 57 (3) ◽  
pp. 431-437 ◽  
Author(s):  
Kamel Chaieb ◽  
Olfa Chehab ◽  
Tarek Zmantar ◽  
Mahmoud Rouabhia ◽  
Kacem Mahdouani ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Effect Of Ph ◽  
Vitro Effect

The in vitro effect of pH on glycosidase activity in potential preys of planktivorous and benthivorous fish

2015 ◽  
Vol 8 (2) ◽  
pp. 182-187
Author(s):  
V. V. Kuz’mina ◽  
G. V. Zolotareva ◽  
V. A. Sheptitsky
Keyword(s):  
Benthivorous Fish ◽  
Effect Of Ph ◽  
Glycosidase Activity ◽  
Vitro Effect

Forage degradability, rumen bacterial adherence and fibrolytic enzyme activityin vitro: effect of pH or glucose concentration

2013 ◽  
Vol 152 (2) ◽  
pp. 325-332 ◽  
Author(s):  
R. FARENZENA ◽  
G. V. KOZLOSKI ◽  
M. P. MEZZOMO ◽  
A. C. FLUCK
Keyword(s):  
Enzyme Activity ◽  
Glucose Concentration ◽  
Specific Activity ◽  
Maximum Activity ◽  
Bacterial Adherence ◽  
Microbial Colonization ◽  
Effect Of Ph ◽  
Glucose Assay ◽  
Vitro Effect

SUMMARYA set of independent assays were conducted to assess the effects of either pH or glucose concentration on forage degradation, bacterial adherence and on fibrolytic enzyme activityin vitro. For measuring degradation and bacterial adherence, ryegrass (Lolium multiflorum) and bermudagrass (Cynodon dactylon) samples were incubatedin vitrofor 24 h in the medium at different pH (5·5, 6·0, 6·5 or 7·0) or with different initial glucose concentrations (0, 1000, 2000 and 3000 mg/l). For fibrolytic enzyme activity evaluation, forage samples were incubatedin situand the extracted enzymes were incubatedin vitrounder the different pH and glucose treatment conditions. The amount of bacteria adhering to samples and the degradability of dry matter (DM) and neutral detergent fibre (NDF) were higher for ryegrass than for bermudagrass, were not affected by glucose concentration and were linearly and positively affected by increased pH. On average, carboxymethylcellulase (CMCase) and xylanase activities were higher for ryegrass than for bermudagrass in the pH assay, whereas the differences between forages were not significant in the glucose assay. For both forage species, the quadratic effect of pH or glucose concentration on CMCase and xylanase activities was significant. Maximum activity of both enzymes was observed at pH 6·0 or at glucose concentration of 2000 mg/l. In conclusion, forage degradation was affected negatively by decreased ruminal pH due to reduced bacterial adherence. In turn, the pH or glucose effect on fibrolytic enzyme activity was not related to their effects on bacterial adherence or forage degradation, indicating that forage degradation is more dependent on the degree of microbial colonization than on the specific activity of bacterial enzymes.

scholarly journals The in vitro effect of pH on osteoclasts and bone resorption in the cat: Implications for the pathogenesis of FORL

2007 ◽  
Vol 213 (1) ◽  
pp. 144-150 ◽  
Author(s):  
Mariusz Muzylak ◽  
Timothy R. Arnett ◽  
Joanna S. Price ◽  
Michael A. Horton
Keyword(s):  
Bone Resorption ◽  
Effect Of Ph ◽  
Vitro Effect

scholarly journals In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production

2005 ◽  
Vol 57 (6) ◽  
pp. 778-783 ◽  
Author(s):  
W.M. Leopoldino ◽  
R.P. Lana ◽  
A.C. Borges ◽  
H.C. Mantovani ◽  
R.M.A. Teixeira ◽  
...  
Keyword(s):  
Protein Production ◽  
Culture Media ◽  
Low Ph ◽  
Potassium Depletion ◽  
Ammonia Production ◽  
Microbial Protein ◽  
Ruminal Bacteria ◽  
Effect Of Ph ◽  
Vitro Effect

Ruminal fluid from steers fed on pasture was incubated with artificial media at pH 5.5 and 7.0 in two experiments. In the first, the effect of monensin level on resistance of ruminal bacteria to potassium depletion was evaluated; in the second, effects of the ionophores monensin and lasalocid on ammonia and protein production were quantified. In experiment 1, culture media affected potassium level. The monensin concentration needed to cause half maximal potassium depletion was 2.77µM at pH 5.5 but was 0.056µM at pH 7.0, showing that bacteria incubated at pH 5.5 were more tolerant to monensin than those incubated at pH 7.0. Both ionophores as well as increased acidity caused decreased ammonia production. Both ionophores inhibited ammonia production by 56%, independently of pH. In cultures incubated at pH 5.5 compared to pH 7.0, ammonia production was decreased by 50.5%, independently of the ionophores. Therefore, effects of ionophores and acidity were additive, and the maximum inhibition occurred in the presence of an ionophore at low pH (75.2%). Microbial protein production was lowest when lasalocid was present in a low pH culture medium, causing inhibition of microbial growth.

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