scholarly journals Viperin mRNA is a novel target for the human RNase MRP/RNase P endoribonuclease

2010 ◽  
Vol 68 (14) ◽  
pp. 2469-2480 ◽  
Author(s):  
Sandy Mattijssen ◽  
Ella R. Hinson ◽  
Carla Onnekink ◽  
Pia Hermanns ◽  
Bernhard Zabel ◽  
...  
Keyword(s):  
Rnase P ◽  
Rnase Mrp ◽  
Human Rnase ◽  
Novel Target

scholarly journals Basic Domains Target Protein Subunits of the RNase MRP Complex to the Nucleolus Independently of Complex Association

2001 ◽  
Vol 12 (11) ◽  
pp. 3680-3689 ◽  
Author(s):  
Hans van Eenennaam ◽  
Annemarie van der Heijden ◽  
Rolf J. R. J. Janssen ◽  
Walther J. van Venrooij ◽  
Ger J. M. Pruijn
Keyword(s):  
Protein Composition ◽  
Rnase P ◽  
Nucleolar Localization ◽  
Rrna Processing ◽  
Protein Subunits ◽  
Trna Processing ◽  
Rnase Mrp ◽  
Human Rnase ◽  
The Difference ◽  
Active Transport Process

The RNase MRP and RNase P ribonucleoprotein particles both function as endoribonucleases, have a similar RNA component, and share several protein subunits. RNase MRP has been implicated in pre-rRNA processing and mitochondrial DNA replication, whereas RNase P functions in pre-tRNA processing. Both RNase MRP and RNase P accumulate in the nucleolus of eukaryotic cells. In this report we show that for three protein subunits of the RNase MRP complex (hPop1, hPop4, and Rpp38) basic domains are responsible for their nucleolar accumulation and that they are able to accumulate in the nucleolus independently of their association with the RNase MRP and RNase P complexes. We also show that certain mutants of hPop4 accumulate in the Cajal bodies, suggesting that hPop4 traverses through these bodies to the nucleolus. Furthermore, we characterized a deletion mutant of Rpp38 that preferentially associates with the RNase MRP complex, giving a first clue about the difference in protein composition of the human RNase MRP and RNase P complexes. On the basis of all available data on nucleolar localization sequences, we hypothesize that nucleolar accumulation of proteins containing basic domains proceeds by diffusion and retention rather than by an active transport process. The existence of nucleolar localization sequences is discussed.

scholarly journals hPop5, a Protein Subunit of the Human RNase MRP and RNase P Endoribonucleases

2001 ◽  
Vol 276 (34) ◽  
pp. 31635-31641 ◽  
Author(s):  
Hans van Eenennaam ◽  
Dorien Lugtenberg ◽  
Judith H. P. Vogelzangs ◽  
Walther J. van Venrooij ◽  
Ger J. M. Pruijn
Keyword(s):  
Rnase P ◽  
Protein Subunit ◽  
Rnase Mrp ◽  
Human Rnase

scholarly journals hPop1: an autoantigenic protein subunit shared by the human RNase P and RNase MRP ribonucleoproteins.

1996 ◽  
Vol 15 (21) ◽  
pp. 5936-5948 ◽  
Author(s):  
Z. Lygerou ◽  
H. Pluk ◽  
W. J. van Venrooij ◽  
B. Séraphin
Keyword(s):  
Rnase P ◽  
Protein Subunit ◽  
Rnase Mrp ◽  
Human Rnase

scholarly journals Heterodimerization of the human RNase P/MRP subunits Rpp20 and Rpp25 is a prerequisite for interaction with the P3 arm of RNase MRP RNA

2010 ◽  
Vol 38 (12) ◽  
pp. 4052-4066 ◽  
Author(s):  
Katherine L. D. Hands-Taylor ◽  
Luigi Martino ◽  
Renée Tata ◽  
Jeffrey J. Babon ◽  
Tam T. Bui ◽  
...  
Keyword(s):  
Rnase P ◽  
Rnase Mrp ◽  
Human Rnase

scholarly journals Multiple cis-acting elements are required for RNA polymerase III transcription of the gene encoding H1 RNA, the RNA component of human RNase P.

1991 ◽  
Vol 266 (34) ◽  
pp. 22796-22799
Author(s):  
G.J. Hannon ◽  
A. Chubb ◽  
P.A. Maroney ◽  
G. Hannon ◽  
S. Altman ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Iii ◽  
Rnase P ◽  
Gene Encoding ◽  
Cis Acting ◽  
Polymerase Iii ◽  
Human Rnase

scholarly journals Fomites and the environment did not have an important role in COVID-19 transmission in a Brazilian mid-sized city

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana Luíza Silva Rocha ◽  
Josilene Ramos Pinheiro ◽  
Thamilin Costa Nakamura ◽  
José Domingos Santos da Silva ◽  
Beatriz Gonçalves Silva Rocha ◽  
...  
Keyword(s):  
Cell Phones ◽  
Rnase P ◽  
Human Cells ◽  
Market Area ◽  
Paper Money ◽  
P Gene ◽  
Human Rnase ◽  
The World ◽  
Reference Hospital

AbstractIt is not clear if COVID-19 can be indirectly transmitted. It is not possible to conclude the role of the environment in transmission of SARS-CoV-2 without studying areas in which people transit in great numbers. In this work we aimed to better understand the role of environment in the spread of COVID-19. We investigated the presence of SARS-CoV-2 in fomites as well as in the air and in the sewage using RT-qPCR. We studied both, a reference market area and a COVID-19 reference hospital at Barreiras city, Brazil. We collected and analyzed a total of 418 samples from mask fronts, cell phones, paper money, card machines, sewage, air and bedding during the ascendant phase of the epidemiological curve of COVID-19 in Barreiras. As a result, we detected the human RNAse P gene in most of samples, which indicates the presence of human cells or their fragments in specimens. However, we did not detect any trace of SARS-CoV-2 in all samples analyzed. We conclude that, so far, the environment and inanimate materials did not have an important role in COVID-19 transmission in Barreiras city. Therefore, similar results can probably be found in other cities, mainly those with COVID-19 epidemiological scenarios similar to that of Barreiras city. Our study is a small piece indicating the possibility that fomites and the environment do not have an important role in COVID-19 transmission. However, further studies are necessary to better understand the world scenario.

scholarly journals Partially Processed pre-rRNA Is Preserved in Association with Processing Components in Nucleolus-derived Foci during Mitosis

1998 ◽  
Vol 9 (9) ◽  
pp. 2407-2422 ◽  
Author(s):  
Miroslav Dundr ◽  
Mark O.J. Olson
Keyword(s):  
Molecular Weight ◽  
Internal Transcribed Spacer ◽  
Rnase P ◽  
Rrna Processing ◽  
Rnase Mrp ◽  
External Transcribed Spacer ◽  
Early Anaphase ◽  
Late Telophase ◽  
Processing Site

Previous studies showed that components implicated in pre-rRNA processing, including U3 small nucleolar (sno)RNA, fibrillarin, nucleolin, and proteins B23 and p52, accumulate in perichromosomal regions and in numerous mitotic cytoplasmic particles, termed nucleolus-derived foci (NDF) between early anaphase and late telophase. The latter structures were analyzed for the presence of pre-rRNA by fluorescence in situ hybridization using probes for segments of pre-rRNA with known half-lives. The NDF did not contain the short-lived 5′-external transcribed spacer (ETS) leader segment upstream from the primary processing site in 47S pre-rRNA. However, the NDF contained sequences from the 5′-ETS core, 18S, internal transcribed spacer 1 (ITS1), and 28S segments and also had detectable, but significantly reduced, levels of the 3′-ETS sequence. Northern analyses showed that in mitotic cells, the latter sequences were present predominantly in 45S-46S pre-rRNAs, indicating that high-molecular weight processing intermediates are preserved during mitosis. Two additional essential processing components were also found in the NDF: U8 snoRNA and hPop1 (a protein component of RNase MRP and RNase P). Thus, the NDF appear to be large complexes containing partially processed pre-rRNA associated with processing components in which processing has been significantly suppressed. The NDF may facilitate coordinated assembly of postmitotic nucleoli.

Sign in / Sign up

Export Citation Format

Share Document