Isolation of cancer stem cells from transformed human mesenchymal stem cell line F6

2010 ◽  
Vol 88 (11) ◽  
pp. 1181-1190 ◽  
Author(s):  
Xuejing Xu ◽  
Hui Qian ◽  
Wei Zhu ◽  
Xu Zhang ◽  
Yongmin Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Mesenchymal Stem Cell ◽  
Cell Line ◽  
Human Mesenchymal Stem Cell ◽  
Stem Cell Line

scholarly journals Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer

2008 ◽  
Vol 12 (4) ◽  
pp. 1347-1359 ◽  
Author(s):  
Wolfgang Böcker ◽  
Zhanhai Yin ◽  
Inga Drosse ◽  
Florian Haasters ◽  
Oliver Rossmann ◽  
...  
Keyword(s):  
Stem Cell ◽  
Gene Transfer ◽  
Mesenchymal Stem Cell ◽  
Single Cell ◽  
Cell Line ◽  
Human Mesenchymal Stem Cell ◽  
Stem Cell Line

Characterization of a novel mesenchymal stem cell line derived from human embryonic stem cells

2016 ◽  
Vol 10 (1) ◽  
pp. 1-9 ◽  
Author(s):  
A. M. Koltsova ◽  
V. V. Zenin ◽  
T. K. Yakovleva ◽  
G. G. Poljanskaya
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Mesenchymal Stem Cell ◽  
Cell Line ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Stem Cell Line

scholarly journals Characterization of a mesenchymal stem cell line that differentiates to bone and provides niches supporting mouse and human hematopoietic stem cells

2012 ◽  
Vol 02 (01) ◽  
pp. 5-14 ◽  
Author(s):  
Sonal R. Tuljapurkar ◽  
John D. Jackson ◽  
Susan K. Brusnahan ◽  
Barbara J. O’Kane ◽  
John G. Sharp
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hematopoietic Stem Cells ◽  
Cell Line ◽  
Stem Cell Line ◽  
Hematopoietic Stem

scholarly journals Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0226538
Author(s):  
Masao Takeuchi ◽  
Kikuko Takeuchi ◽  
Tomoyo Takai ◽  
Ritsuko Yamaguchi ◽  
Tetsushi Furukawa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Migration ◽  
Mesenchymal Stem Cell ◽  
Cell Line ◽  
Primary Cilia ◽  
Growth Factor Receptor ◽  
Leading Edge ◽  
Human Mesenchymal Stem Cell ◽  
Small Gtpases ◽  
Stem Cell Line

Glypican-5 (GPC5) is a heparan sulfate proteoglycan (HSPG) localized to the plasma membrane. We previously reported that in the human mesenchymal stem cell line UE6E7T-3, GPC5 is overexpressed in association with transformation and promotes cell proliferation by acting as a co-receptor for Sonic hedgehog signaling. In this study, we found using immunofluorescence microscopy that in transformed cells (U3DT), GPC5 localized not only at primary cilia on the cell surface, but also at the leading edge of migrating cells, at the intercellular bridge and blebs during cytokinesis, and in extracellular vesicles. In each subcellular region, GPC5 colocalized with fibroblast growth factor receptor (FGFR) and the small GTPases Rab11 and ARF6, indicating that GPC5 is delivered to these regions by Rab11-associated recycling endosomes. These colocalizations suggest that GPC5 plays an important role in FGF2 stimulation of cell migration, which was abrogated by knockdown of GPC5. Our findings indicate that GPC5 plays a role in regulation of U3DT cell migration and provides several insights into the functions of GPC5 that could be elucidated by future studies.

scholarly journals Subcellular localization of glypican-5 associates with dynamic cell motility and cell communication of the human mesenchymal stem cell line U3DT

2019 ◽  
Author(s):  
Masao Takeuchi ◽  
Kikuko Takeuchi ◽  
Yoko Monobe ◽  
Tomoyo Takai ◽  
Ritsuko Yamaguchi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Line ◽  
Primary Cilia ◽  
Leading Edge ◽  
Human Mesenchymal Stem Cell ◽  
Small Gtpase ◽  
Stem Cell Line ◽  
Subcellular Compartment

AbstractGlypican-5 (GPC5) is a heparan sulfate proteoglycan (HSPG) localized to the plasma membrane. We previously reported that in the human mesenchymal stem cell line UE6E7T3, GPC5 is overexpressed in association with transformation and promotes cell proliferation by acting as a co-receptor for Sonic hedgehog signaling. In this study, we found using an immunofluorescence microscopy that in transformed cells (U3DT), GPC5 localized not only at primary cilia on the cell surface, but also at the leading edge of migrating cells, at the intercellular bridge and blebs during cytokinesis, and in extracellular vesicles. In each subcellular region, GPC5 colocalized with the small GTPase Rab11. These observations suggest that the colocalization of GPC5 with Rab11 is crucial for its function, and that its activity in each subcellular compartment promotes proliferation of U3DT cells. Our findings indicate that GPC5 plays active and essential roles in regulation of U3DT cell proliferation, and provides several insights into the functions of GPC5 that could be elucidated by future studies.

scholarly journals Multilineage Potential of Stable Human Mesenchymal Stem Cell Line Derived from Fetal Marrow

PLoS ONE ◽  
2007 ◽  
Vol 2 (12) ◽  
pp. e1272 ◽  
Author(s):  
Atsushi Nagai ◽  
Woo K. Kim ◽  
Hong J. Lee ◽  
Han S. Jeong ◽  
Kwang S. Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Line ◽  
Human Mesenchymal Stem Cell ◽  
Stem Cell Line ◽  
Multilineage Potential

scholarly journals Interfacing Sca-1posMesenchymal Stem Cells with Biocompatible Scaffolds with Different Chemical Composition and Geometry

2009 ◽  
Vol 2009 ◽  
pp. 1-10 ◽  
Author(s):  
G. Forte ◽  
O. Franzese ◽  
S. Pagliari ◽  
F. Pagliari ◽  
A. M. Di Francesco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Chemical Composition ◽  
Mesenchymal Stem Cell ◽  
Cell Line ◽  
Stem Cell Line ◽  
Self Renewal ◽  
Single Cell Isolation ◽  
Functional Features ◽  
Multilineage Potential

An immortalized murine mesenchymal stem cell line (mTERT-MSC) enriched forLinneg/Sca-1posfraction has been obtained through the transfection of MSC with murine TERT and single-cell isolation. Such cell line maintained the typical MSC self-renewal capacity and continuously expressed MSC phenotype. Moreover, mTERT-MSC retained the functional features of freshly isolated MSC in culture without evidence of senescence or spontaneous differentiation events. Thus, mTERT-MSC have been cultured onto PLA films, 30 and 100 μm PLA microbeads, and onto unpressed and pressed HYAFF-11 scaffolds. While the cells adhered preserving their morphology on PLA films, clusters of mTERT-MSC were detected on PLA beads and unpressed fibrous scaffolds. Finally, mTERT-MSC were not able to colonize the inner layers of pressed HYAFF-11. Nevertheless, such cell line displayed the ability to preserve Sca-1 expression and to retain multilineage potential when appropriately stimulated on all the scaffolds tested.

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