Identification of consistent QTL with large effect on anther extrusion in doubled haploid populations developed from spring wheat accessions in German Federal ex situ Genebank

2019 ◽  
Vol 132 (11) ◽  
pp. 3035-3045 ◽  
Author(s):  
Quddoos H. Muqaddasi ◽  
Murukarthick Jayakodi ◽  
Andreas Börner ◽  
Marion S. Röder
2007 ◽  
Vol 55 (3) ◽  
pp. 273-282
Author(s):  
S. Sharma ◽  
H. Chaudhary

Seventy-eight doubled haploid (DH) lines, derived from 21 elite and diverse winter × spring wheat F 1 hybrids, following the wheat × maize system, were screened along with the parental genotypes under in vitro and in vivo conditions for cold tolerance. Under in vitro conditions, the 2,3,5-triphenyl tetrazolium chloride (TTC) test was used to characterize the genotypes for cold tolerance. Based on the TTC test, only one doubled haploid, DH 69, was characterized as cold-tolerant, seven DH and five winter wheat parents were moderately tolerant, while the rest were susceptible. Analysis of variance under in vivo conditions also indicated the presence of sufficient genetic variability among the genotypes (DH lines + parents) for all the yield-contributing traits under study. The correlation and path analysis studies underlined the importance of indirect selection for tillers per plant, harvest index and grains per spike in order to improve grain yield. It was also concluded that selection should not be practised for grain weight per spike as it would adversely affect the grain yield per plant. When comparing the field performance of the genotypes with the in vitro screening parameters, it was concluded that in addition to the TTC test, comprising a single parameter, other physiological and biochemical in vitro parameters should be identified, which clearly distinguish between cold-tolerant and susceptible genotypes and also correlate well with their performance under field conditions.


2020 ◽  
Vol 48 (4) ◽  
pp. 525-532
Author(s):  
Mohamed N. Barakat ◽  
Abdullah A. Al-Doss ◽  
Khaled A. Moustafa ◽  
Mohamed I. Motawei ◽  
Mohamed S. Alamri ◽  
...  

2009 ◽  
Vol 89 (5) ◽  
pp. 945-951 ◽  
Author(s):  
R M DePauw ◽  
R E Knox ◽  
F R Clarke ◽  
J M Clarke ◽  
T N McCaig

Based on 34 replicated trials over 3 yr, Stettler, a doubled haploid hard red spring wheat (Triticum aestivum L.), expressed significantly higher grain yield than all checks except Superb. Wheat and flour protein concentration were significantly greater than all of the checks except Lillian. It matured significantly later than AC Barrie and Katepwa but earlier than Superb. Stettler was significantly shorter than all of the checks except Superb and was more resistant to lodging than Katepwa and Laura. Stettler had high grain volume weight and intermediate kernel weight relative to the checks, and meets the end-use quality specifications of the Canada Western Red Spring wheat market class. Stettler expressed resistance to prevalent races of stem rust, common bunt and loose smut, with moderate susceptibility to prevalent races of leaf rust and fusarium head blight.Key words: Triticum aestivum L., cultivar description, grain yield, protein, disease resistance, doubled haploid


Agronomy ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 407 ◽  
Author(s):  
Quddoos H. Muqaddasi ◽  
Jochen C. Reif ◽  
Marion S. Röder ◽  
Bhoja R. Basnet ◽  
Susanne Dreisigacker

Hybrid breeding facilitates the exploitation of heterosis and it can result in significant genetic gains and increased crop yields. Inefficient cross-pollination is a major limiting factor that hampers hybrid wheat seed production. In this study, we examined the genetic basis of anther extrusion (AE), which is an important trait in increasing cross-pollination, and thus improving seed set on the female lines and hybrid wheat seed production. We studied 300 segregating F2 plants and F2:3 families that result from a cross of two elite spring wheat lines. We observed that F2 and F2:3 populations hold significant and continuous genetic variation for AE, which suggests its reliable phenotypic selection. Composite interval mapping detected three quantitative trait loci (QTL) on chromosomes 3A, 5A, and 5D. The QTL on chromosome 5A (i.e., QAe.cimmyt-5A) was of large-effect, being consistently identified across generations, and spanned over 25 cM. Our study shows that (1) AE possesses strong genetic control (heritability), and (2) the QTL QAe.cimmyt-5A that imparted on an average of 20% of phenotypic variation can be used for marker-assisted selection (MAS) in breeding programs. In addition, pyramiding the large-effect QTL for MAS could efficiently complement the phenotypic selection since it is relatively easy and cheap to visually phenotype AE. This study reports the first large-effect QTL for AE in spring wheat, endorsing the use of this analysis in current hybrid wheat breeding and future Mendelization for the detection of underlying gene(s).


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