scholarly journals Oxidant regulation of gene expression and neural tube development: Insights gained from diabetic pregnancy on molecular causes of neural tube defects

Diabetologia ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 538-545 ◽  
Author(s):  
T. I. Chang ◽  
M. Horal ◽  
S. K. Jain ◽  
F. Wang ◽  
R. Patel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Regulation Of Gene Expression ◽  
Diabetic Pregnancy ◽  
Neural Tube Development

scholarly journals Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord

2018 ◽  
Author(s):  
Julien Delile ◽  
Teresa Rayon ◽  
Manuela Melchionda ◽  
Amelia Edwards ◽  
James Briscoe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Single Cell ◽  
Neural Tube ◽  
Temporal Dynamics ◽  
Regulation Of Gene Expression ◽  
Cell Types ◽  
Neuronal Diversity ◽  
Systematic Analysis ◽  
Neural Tube Development

ABSTRACTThe coordinated spatial and temporal regulation of gene expression in the vertebrate neural tube determines the identity of neural progenitors and the function and physiology of the neurons they generate. Progress has been made deciphering the gene regulatory programmes responsible for this process, however, the complexity of the tissue has hampered the systematic analysis of the network and the underlying mechanisms. To address this, we used single cell mRNA sequencing to profile cervical and thoracic regions of the developing mouse neural tube between embryonic days (e)9.5-e13.5. We confirmed the data accurately recapitulates neural tube development, allowing us to identify new markers for specific progenitor and neuronal populations. In addition, the analysis highlighted a previously underappreciated temporal component to the mechanisms generating neuronal diversity and revealed common features in the sequence of transcriptional events that lead to the differentiation of specific neuronal subtypes. Together the data provide a compendium of gene expression for classifying spinal cord cell types that will support future studies of neural tube development, function, and disease.

Melatonin prevents neural tube defects in the offspring of diabetic pregnancy

2015 ◽  
Vol 59 (4) ◽  
pp. 508-517 ◽  
Author(s):  
Shangming Liu ◽  
Yuji Guo ◽  
Qiuhuan Yuan ◽  
Yan Pan ◽  
Liyan Wang ◽  
...  
Keyword(s):  
Neural Tube ◽  
Neural Tube Defects ◽  
Diabetic Pregnancy

Inhibition of methylation and changes in gene expression in relation to neural tube defects

2008 ◽  
Vol 82 (10) ◽  
pp. 676-683 ◽  
Author(s):  
Ivon J. M. van der Linden ◽  
Sandra G. Heil ◽  
Michael van Egmont Petersen ◽  
Henny W. van Straaten ◽  
Martin den Heijer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Neural Tube ◽  
Neural Tube Defects

scholarly journals Protein kinase C-alpha suppresses autophagy and induces neural tube defects via miR-129-2 in diabetic pregnancy

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Fang Wang ◽  
Cheng Xu ◽  
E. Albert Reece ◽  
Xuezheng Li ◽  
Yanqing Wu ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Diabetic Pregnancy ◽  
Kinase C ◽  
Protein Kinase C Alpha

scholarly journals Gene Expression Profile Associated With Neural Tube Defects Among Infants Attending Muhimbili National Hospital And Muhimbili Orthopaedic Institute In Tanzania

2020 ◽  
Author(s):  
Mariam Sadallah ◽  
Evelyne Neema Assenga ◽  
Charles Pallangyo ◽  
Karim Manji
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Statistical Tests ◽  
Significant Risk ◽  
Muhimbili National Hospital ◽  
National Hospital ◽  
Low Expression

Abstract Background: Neural tube defects (NTDs) are severe congenital anomalies of the central nervous system. The specific cause is not known, though several factors including gene variants involving the folate metabolism have been implicated in the etiology. This study aimed at identifying the gene expression profile of selected genes known to be associated with NTDs among infants attending Muhimbili National Hospital (MNH) and Muhimbili Orthopaedic Institute (MOI).Methodology: We conducted a matched case control study involving infants who were attending MNH and MOI during 6 months of the study period. Each case was matched with two controls by gestational age, sex and birth weight. Whole blood samples were collected from all study participants for genetic analysis. Total RNA was isolated using Qiagen RNA blood mini kit, and reversed transcribed into complementary DNA (cDNA) using Super Script II Reverse Transcriptase cDNA Synthesis kit. Real-time polymerase chain reaction was performed on extracted cDNA by a Light Thermal Cycler 480 machine using specific primers for studied genes to determine their expression levels. Results were analysed by GraphPad Prism 5Software, using Student t-test, and Bonferroni post hoc statistical tests. A p value of < 0.05 was considered to be statistically significant.Results: The study recruited 50 cases and 100 controls. Among eight studied genes, we found significantly low expression of Methylenetetrahydrofolate reductase [MTHFR] gene among cases than controls (p=0.006). Expression of others genes were having variations and expressed at very low levels in both cases and controls.Conclusion: A low expression level of MTHFR gene was a significant risk factor for the occurrence of NTDs amongst infants attending MNH and MOI.Recommendation: We recommend gene expression analysis to be done on mothers of infants with NTDs to identify those at risk; and further mutational analysis to be done to enhance genetic counseling and therefore to prevent recurrence of NTDs.

Epigenetic Mechanisms: An Introduction for the NICU Nurse

2019 ◽  
Vol 38 (5) ◽  
pp. 278-285
Author(s):  
Susan Braid ◽  
Daniel E. Zelac
Keyword(s):  
Gene Expression ◽  
Dna Sequence ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Epigenetic Mechanisms ◽  
Cardiovascular Defects

Our understanding of epigenetics and the mechanisms of gene expression has grown rapidly, requiring the NICU nurse to understand the implications on a neonate's health. Epigenetics is heritable changes of the gene expression without changing the DNA sequence. Though most of the etiologies of birth disorders remain unknown, they could result from interactions of genetics, lifestyle, and environment that make changes in the epigenome landscape. Epigenetics may help explain disorders neonates express including cardiovascular defects, cleft lip and palate, and neural tube defects. As our understanding of epigenetics improves, NICU nurses will need to understand possible epigenetic implications on the neonate's health so as to provide better care and guidance to families.

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