scholarly journals The polymorphisms of extracellular matrix-remodeling genes are associated with pelvic organ prolapse

2022 ◽  
Author(s):  
Lei Li ◽  
Yidi Ma ◽  
Hua Yang ◽  
Zhijing Sun ◽  
Juan Chen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Pelvic Organ Prolapse ◽  
Pelvic Organ ◽  
Matrix Remodeling ◽  
Nucleotide Polymorphisms ◽  
Processing Enzymes ◽  
Tissue Inhibitors Of Metalloproteinase ◽  
Genes Encoding ◽  
A Disintegrin And Metalloproteinase ◽  
Adjusted Model

Abstract Introduction and hypothesis Extracellular matrix (ECM) synthesis and metabolism abnormalities may influence the pelvic supporting system and lead to the occurrence and development of pelvic organ prolapse (POP). Genetic polymorphisms of such related genes have been increasingly studied. This study aims to explore the association between the single-nucleotide polymorphisms (SNPs) of genes encoding ECM processing enzymes (a disintegrin and metalloproteinase with thrombospondin motifs [ADAMTSs]), ECM degrading enzymes (matrix metalloproteinases [MMPs]) and their tissue inhibitors of metalloproteinase (TIMPs), and POP. Methods We conducted an association study including 48 women with POP at stages III and IV and 48 women without prolapse in Chinese groups. SNPs were identified using the target region sequencing technique. We performed Fisher’s exact tests to assess the association between SNPs and POP in the unadjusted model and logistic regression analysis in the adjusted model, adjusting for delivery and pregnancy. Results There was a significant association between TIMP2 SNP rs2277698 (odds ratio [OR], 0.37; 95% confidence interval [CI], 0.16–0.82; P = 0.015), ADAMTS13 SNP rs149586801 (OR, 0.18; 95% CI, 0.05–0.69; P = 0.012), and ADAMTS1 SNPs rs370850 and rs422803 (OR, 3.71; 95% CI, 1.35–10.15; P = 0.011 for both), rs402007, rs428785, rs434857, and rs445784 (OR, 2.18; 95% CI, 1.05–4.56; P = 0.038 for the four), and POP in the adjusted model. Conclusion TIMP2, ADAMTS13, and ADAMTS1 might be candidate genes for POP. Our results provide preliminarily new evidence for future investigation of these genes in the pathophysiology of POP.

scholarly journals Collagen type 1A1, type 3A1, and LOXL1/4 polymorphisms as risk factors of pelvic organ prolapse

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Asuka Ashikari ◽  
Tetsuji Suda ◽  
Minoru Miyazato
Keyword(s):  
Risk Factors ◽  
Pelvic Organ Prolapse ◽  
Collagen Type ◽  
Univariate Analysis ◽  
Multivariate Logistic Regression Analysis ◽  
Pelvic Organ ◽  
Japanese Women ◽  
Control Group ◽  
Matrix Remodeling ◽  
Nucleotide Polymorphisms

Abstract Objective Collagen and elastin are the main components of the female pelvic tissue. We investigated whether single nucleotide polymorphisms (SNPs) of collagen type 1 alpha 1 (COL1A1), collagen type 3 alpha 1 (COL3A1), and lysyl oxidase-like (LOXL) 1 and 4 were associated with the onset of pelvic organ prolapse (POP) in Japanese women. Fifty-two women with POP and 28 women without POP were included. SNPs were identified using the TaqMan® SNP genotyping assay. Results Age, parity, and lower urinary tract symptoms were significantly higher in the POP group than in the control group. The prevalence of genotypes with rs2862296 polymorphism of LOXL4, an enzyme essential for extracellular matrix remodeling, was different between the POP (26.9% for GG, 51.9% for AG) and control groups (14.8% for GG, 33.3% for AG). However, polymorphisms of COL1A1, COL3A1, and LOXL1 were not related to the onset of POP. In the multivariate logistic regression analysis, age was significantly associated with the occurrence of POP. In the univariate analysis, LOXL4 polymorphism was associated with the onset of POP in Japanese women. The knowledge of acquired risk factors and polymorphisms in the genomic background of patients with POP may help prevent POP via early conservative interventions.

Expression of extracellular matrix and adhesion proteins in pelvic organ prolapse

2018 ◽  
Vol 64 (5) ◽  
pp. 142 ◽  
Author(s):  
Monica Cecati ◽  
Alessandra Corradetti ◽  
Davide Sartini ◽  
Valentina Pozzi ◽  
Stefano R Giannubilo ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Pelvic Organ Prolapse ◽  
Pelvic Organ ◽  
Adhesion Proteins

scholarly journals Dose-Dependent Antifibrotic Effect of Chrysin on Regression of Liver Fibrosis: The Role in Extracellular Matrix Remodeling

Dose-Response ◽  
2018 ◽  
Vol 16 (3) ◽  
pp. 155932581878983 ◽  
Author(s):  
Cornel Balta ◽  
Alina Ciceu ◽  
Hildegard Herman ◽  
Marcel Rosu ◽  
Oana Maria Boldura ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Liver Fibrosis ◽  
Mouse Liver ◽  
Ultrastructural Changes ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Tissue Inhibitors Of Metalloproteinase ◽  
Spontaneous Reversion ◽  
New Strategy ◽  
Dose Dependent

Liver fibrosis represents an overaccumulation of extracellular matrix (ECM). This study was designed to investigate the effect of chrysin on established ECM overproduction in carbon tetrachloride (CCl4) mouse liver fibrosis. Experimental fibrosis was induced by intraperitoneal injection of 2 mL/kg CCl4 twice a week, for 7 weeks. Mice were orally treated with 3 doses of chrysin (5,7-dihydroxyflavone). For the assessment of the spontaneous reversion of fibrosis, CCl4-treated mice were investigated after 2 weeks of recovery time. Silymarin was used as a standard of liver protection. In fibrotic livers, the results showed the upregulation of collagen I (Col I) and tissue inhibitors of metalloproteinase 1 (TIMP-1) and modulation of matrix metalloproteinases (MMPs), which led to an altered ECM enriched in Col, confirmed as well by electron microscopy investigations. Treatment with chrysin significantly reduced ultrastructural changes, downregulated Col I, and restored TIMP-1/MMP balance, whereas in the group observed for the spontaneous regression of fibrosis, they remained in the same pattern with fibrotic livers. In this study, we have shown chrysin efficacy to attenuate dose-dependent CCl4-stimulated liver ECM accumulation by regulation of MMP/TIMP imbalance and inhibition of Col production. We have shown the dose-dependent chrysin efficiency in attenuation of CCl4-induced liver ECM accumulation by regulation of MMP/TIMP imbalance and inhibition of Col production. Our findings suggest that chrysin oral administration may introduce a new strategy for treating liver fibrosis in humans.

Sign in / Sign up

Export Citation Format

Share Document